YOKOGAWA CQ1, User Manual, Page: 23 / 268

<1. Function Overview>
1-3
IM 80J01A01-01E
1.2 Principle of the Nipkow Disk System with Micro-lens
Two disks, including the “pinhole array disk” having many pinholes
arranged
in a helical pattern, and the “Micro-lens array disk” that
condenses excitation laser to individual pinholes, are rotated jointly at
high speed to perform multiple scans over the observation area with
approximately 1,000 laser beams.
Multiple beam scans are performed not only at high speed, but also
with each beam exciting fluorochromes at high efficiency and very low
laser intensity. These results in an optimal live cell observation system
where phototoxicity and fluorescence photobleaching are suppressed
notably compared to any conventional systems while allowing
high-resolution real confocal imaging.
Operating Principle of the Micro-lens enhanced dual Nipkow Disk
System