Manual
Low pressure chromatography column
Pilot series
These instructions should be read prior to using the column
Page 1: ...Manual Low pressure chromatography column Pilot series These instructions should be read prior to using the column...
Page 2: ...h and safety and general safety regulations apply to the use of the product The following laws and standards were taken into account in the creation of this manual 2001 95 EG DIN EN 82 079 1 VDI 4500...
Page 3: ...3 Specifications 14 3 4 Chemical resistance 15 3 5 General operations 17 3 5 1 Tightening and loosening of the O ring 17 3 5 2 Adjusting the stamp height 17 3 5 3 Connecting the tubing 17 3 6 Dismant...
Page 4: ...3 Notes for Cleaning In Place CIP 39 4 4 Unpacking 40 4 4 1 Procedure when moving the upper piston 40 4 4 2 Procedure when using liquid flow 40 5 Maintenance and care 41 5 1 Maintenance plan 41 5 2 R...
Page 5: ...coloured signal word fields Always read the full text of the warning in order to effectively protect yourself from danger The following signal word fields marked by different colors and signal words...
Page 6: ...ary phases This ensures the best requirements for a high recovery of the analytes The properties of the biomolecules for example proteins oligosaccharides or nucleic acids remain unchanged The columns...
Page 7: ...advice and terms in the manual The product is only for the use with liquids that have a pressure 0 5 bar above the atmospheric pressure at the maximum temperature of 40 C 1 5 Personnel requirements T...
Page 8: ...de and to identify the column via serial number Therefore you can get individual support if needed The product code includes information of inner diameter length frit porosity and the solvent resistan...
Page 9: ...part number PI100 500V 0 E AB FDA ID 100 mm column length glass body 500 mm frit porosity 10 m O ring EPDM type aqueous buffer feature FDA certified For custom made Pilot columns please contact YMC Eu...
Page 10: ...ents uchapter 2 1 page 9 3 Verifying the delivery of visible transport damage Transport damages u Procedure if you recognise visible transport damage 1 Refusal or reserved acceptance of the delivery 2...
Page 11: ...ions Otherwise there is a risk of injury and material damage CAREFUL The temperature range which is used may not exceed fall below the given specifications WARNING Make sure that the wheel brakes are...
Page 12: ...Pilot column Picture can differ from product supplied 1 glassbody 2 rotatable rack 3 adjustment knob 4 internal spindel 5 external spindel 6 nut of internal spindel 7 nut of external spindel 8 column...
Page 13: ...s similar to each other The lower pis ton is shorter than the upper one and has no adjustment knob 3 The pistons consist of an inner spindle 4 and an outer spindle 5 with inlet or outlet Further more...
Page 14: ...ibrated precision glass Duran KPG Frits stainless steel 10 m Sealings Kalrez O rings Connection stainless steel capillaries AD Wetted parts borosilicate glass 3 3 Kalrez stainless steel PTFE ID mm Ver...
Page 15: ...ely free of metals As a result most saline buffer solutions can be used without hesitation i The aqueous buffer AB version has only limited resistance to organic solvents Before selecting organic solv...
Page 16: ...te good good good good good good good good good good Cyclohexane good good good unstable good limited good good good good Dichlormethane limited good good unstable good unstable good unstable limited...
Page 17: ...adjustment knob 3 2 Once the height has been set the O ring 14 must be tightened again uchapter 3 5 1 3 5 3 Connecting the tubing u Connecting the tubing 1 Drive the screws please consider torque tab...
Page 18: ...lly Remove the plunger and put it with the frit down on a clean padded surface 3 Tighten the O ring 14 of the lower plunger uchapter 3 5 1 page 17 4 Remove the screws 15 on the lower end plate 9 which...
Page 19: ...hers 17 at the upper column disc 18 ATTENTION After removal of the column disc the glass body is exposed Make sure that it will not fall over 9 Lift the column disc 18 from the column body The O ring...
Page 20: ...is order 13 Pull the inner spindle 4 in the direction of the piston out of the outer spindle 5 14 The piston 11 is screwed onto the inner spindle 6 and can be screwed off now by hand 15 Loosen the tub...
Page 21: ...ve the glass body as described in uchapter 3 6 2 page 19 20 Remove the countersunk screws 23 Af terwards the frit 13 the O ring 24 and the distributor plate 25 can be removed 21 Pull off the tubing of...
Page 22: ...uitable solvent and a lint free cloth 3 Clean the piston with a suitable solvent and or pump 1 M sodium hydroxide so lution through the plunger For thorough cleaning the column can be completely disma...
Page 23: ...screw the piston with the inner spindle and tighten it by hand 7 Pull the outer spindle 5 on the inner spindle 4 Align the notch at the bottom of the outer spindle to the pins of the pressure plug 12...
Page 24: ...1 is placed concentrically on the upper surface of the glass tube The upper columns disc 18 is placed concentrically over the O ring The threads of rods 33 attached to the lower column disc pass throu...
Page 25: ...ng and crosswise pro cedure Go crosswise following the nu merical order shown in the fi gure i When tightening the last cap nuts 16 the torque of the adjacent screwed cap nuts can become reduced There...
Page 26: ...ension nut i When working with large columns it is advisable to work with two persons since the plunger has a considerable weight A column with a pivot frame can be rotated This facilitates the assemb...
Page 27: ...the column this has been tested according to the standard pro cedure listed below The relevant certificate is located in the documentation folder WARNING Never put the glass column under gas pressure...
Page 28: ...used to reach the corresponding maximum pressure 6 Close both gate valves stop the pump and hold the corresponding maximum pressure for 10 minutes If no leaks or damage are observed the test has been...
Page 29: ...the de signated notch on the column tube 3 Bolt the packing adapter by placing the washers 17 on the tie rods Fasten with the cap nuts 40 with which the column disc 35 was previously held in position...
Page 30: ...pressure Operate the column only with pressures up to the stated specifications otherwise there is a risk of material damage or even injury to the user ATTENTION Particles in the solvent can lead to c...
Page 31: ...column is stable Otherwise the column may be damaged uchapter 3 4 page 15 chemical resistance WARNING Rotatable glass body Severe injuries due to uncontrolled rotation of the column body u Always rot...
Page 32: ...ntation process which is described below Column packing with applied flow is also possi ble This method is described on page 37 more accurately After column packing a column test should be performed 4...
Page 33: ...2 Mix the slurry with a spoon or a stirring rod until it is homogenous Air bubble formation should be avoided 3 Degas the homogeneous slurry in an ultrasonic bath 4 Allow the slurry to settle and if p...
Page 34: ...ls such as silica or alumina based particles 1 Pour the homogeneous and degassed slurry rapidly into the column but avoid the introduction of air bubbles Rinse the inner column wall with the same solv...
Page 35: ...ll solvent exits the up per piston Make sure that there is no more air in the system 4 Connect the pump as quickly as possible at the upper piston inlet Remove the closing plug or open the valve at th...
Page 36: ...ng material and column The flow rate during packing should be considerably higher 20 than the flow rate to be used for separations 6 After switching off the pump disconnect the pump and move the pisto...
Page 37: ...ubbles Wash the inner column wall with the same solvent to re move any residual packing material 2 The column should not be moved any more until the packing process has finished in order to avoid dist...
Page 38: ...erial 8 Open the column outlet 9 Equilibrate the column with the appropriate eluent 10 Finally test the column using the appropriate conditions 4 2 5 Column test and release criteria The column test a...
Page 39: ...utions Depyrogenation includes the removal of endotoxins which can be bound on the chromatographic material or the column hardware frits tubing etc and which can contaminate the purified product Befor...
Page 40: ...ing a complete disassembly and cleaning of the glass column is recom mended uchapter 6 3 p 18 chapter 6 7 p 24 6 For further cleaning the movable plunger can also be removed in this position ac cordin...
Page 41: ...eck frits and replace frits if necessary uchapter 5 2 page 42 frit replacement WARNING Some chemicals used for cleaning can result in serious injury Ple ase perform the cleaning of the chromatographic...
Page 42: ...aintenance and care Troubleshooting 5 2 Replacement of wearing parts For the replacement of frits glass body and O rings please follow the steps in uchapter 3 6 page 18 dismantling and uchapter 3 8 pa...
Page 43: ...rer of the packing ma terial uchapter 4 3 p 39 CIP 5 Replace packing material 3 Air in the column degassing or evaporation of solvent during storage New column conditioning Possibly new packing materi...
Page 44: ...t the manufacturer should be considered 7 2 Packing and transport We recommend you use the original wooden box for transport If you are no longer in possession of the box please pack the column in the...
Page 45: ...order number For a better understanding an example of the generation of the order number PI100 500V0E AB FDA is shown below This contains the information for a pilot column with a maximum bed length...
Page 46: ...might also be interested in i i ECOPLUS laboratory glass columns Especially pressure resistant alternative to ECO columns up to 80 bar Practical bayonet locking mechanism 5 to 50 mm ID Easy handling t...
Page 47: ...sizes 10 microns 15 microns 20 microns 50 microns 75 microns 150 microns Pore sizes 8 nm 12 nm 20 nm 30 nm pH and temperature stable hybrid material YMC Triart Prep YMC DAU columns YMC Actus Column t...
Page 48: ...Cleaning in place CIP 39 D Delivery contents 8 Dismantling 18 Disposal 40 F Frit replacement 21 P Packaging 44 Packing 30 Packing adapter 29 Packing procedures 32 Plunger adjustment 17 Pressure test...
Page 49: ...ressure chromatography column Pilot series Appendix 12 Appendix Logbook Activity No Date Activity for example maintenance cleaning and more and or fault description Measures taken Perfor med by Result...
Page 50: ...50 Low pressure chromatography column Pilot series Activity No Date Activity for example maintenance cleaning and more and or fault description Measures taken Perfor med by Result comment Appendix...
Page 51: ...51 Low pressure chromatography column Pilot series Activity No Date Activity for example maintenance cleaning and more and or fault description Measures taken Perfor med by Result comment Appendix...
Page 52: ...o 13 B District Centre Mayur Vihar Phase I New Delhi 110091 India PHONE 91 11 45041601 45041701 FAX 91 11 45041901 www ymcindia com Korea Co Ltd 310 Woolim W City 9 22 Pangyo ro 255 Beon gil Bundang g...
