YL Instruments YL9100 HPLC, User Manual

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YL9110 Quaternary Pump
4-3-2. Filtration
Y
L9110
Solvent should be necessarily filtered through 0.45
μ
m or less filtering membrane before use. Removal
of small particles is necessary to compensate reliable operation of piston seal, and is necessary measure
for reliability of other components in liquid chromatograph.
Filtration process is necessary after mixing of solvent, and is more necessary in case of buffer to which
un-dissolved impurities are source of deposits. After filtration, solvent should be keep in air-tight bottle
from which small particles are removed; once solvent has been filtered, it is not necessary to filter this
solvent everyday unless reaction produce bacteria or indissoluble material occurs. If solvent is kept in
storage vessel for more than one week, it is desired to filter it again before use.
4-3-3. Solvent effect on the instrument
All parts of the Gradient Pump contacting with mobile phase is manufactured from 316 stainless steel,
ruby, sapphire, zirconium, or fluorine carbon polymer. Most of these materials are sensitive to chloride,
and it is desired to avoid use of solvent which contains even small quantity of chloride. Main solvents that
should be avoided especially are as follows.
Aqua Regia Hydrochloric Acid(HCL) (20%)
Bromine HCL (37%)
Chlorine Anhydrous HCL (50%)
Copper Chloride HCL (20%)
Ferric Chloride HCL (75%)
Ferrous Chloride Hydrofluorsilicic Acid (20%)
Freon 12 Hydrogen Peroxide
Guanidine Lodine
Hydrochloride (6M) Mercuric Chloride
Hydrobromic (20%) (Dilute Solution)
In addition, it should be avoided to leave chloroform, carbon tetrachloride, etc. in instrument for long
time, and use of ammonium hydroxide should be avoided because it has effect on stator and rotor of
injector even though it has no effect on pump. When not using it for long time, keep it with iso-propanol
filled with in flow path.
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