2
XCell
TM
ATF System with C410:V3 Controller
User Guide
Figure 1. Filtration Assembly connection to C410:V3 Controller and a bioreactor side port
1.1
XCell
TM
ATF System Pump Cycle
The Diaphragm Pump is the heart of the ATF process. It produces an alternating flow
through the HFM (lumen side) or SM. The XCell
TM
ATF System provides a pulsating,
reversible, flow of liquid, back and forth, between the process vessel and the Diaphragm
Pump. The following is a description of that process:
The Diaphragm Pump is partitioned into two chambers with a flexible diaphragm, Figure
2. One of the pump chambers, the
Pump Liquid
(PL)
chamber
is connected to the Filter
Housing, which, in turn, is connected to the process vessel. Therefore, any flow between
the Diaphragm Pump and process vessel will be through the filtration device. The
second pump chamber, the
Pump Air
(PA)
chamber,
is connected to the pump flow
control system. Typically, controlled addition of compressed air into the PA chamber
increases the pressure in the chamber relative to the process vessel, forcing the flexible
diaphragm partitioning the two chambers to move into the PL chamber and towards the
vessel. Liquid in the PL chamber is forced through the filter to the process vessel. The
flow through the HFM (lumen side) generates tangential flow in one direction. This
pumping phase (or cycle) is called the Pressure Cycle. Inversely, with a pressurized
process vessel relative to PA or PL, or with an external vacuum supply, liquid will flow in
the reverse direction, from process vessel, through the HFM (lumen side), to the PL
chamber, generating tangential flow in the other direction. This pumping phase (or
cycle) is called the Exhaust Cycle. These alternating pump cycles are then repeated
continuously. See also Figure 2.
