46
XCell
TM
ATF System with C410:V3 Controller
User Guide
SOL1
INCOMING AIR
EXHAUST (VACUUM)
PROPORTIONAL VALVE
PV1
PRESSURE
SENSOR
P2
TO PUMP
PROPORTIONAL VALVE
PV2
AUTOMATIC
PRESSURE
REGULATOR
PRV1
MANUAL
PRESSURE
REGULATOR
AUTOMATIC
VACUUM
REGULATOR
PRV2
controller will continually adjust the Pressure and Exhaust flow rates to match the
entered set point flow rate.
Figure 7.
Instrument Flow Control Schematic of C410:V3Controller
XCell™ ATF System Flow control is achieved by regulating the pneumatic air flow to and
from the Diaphragm Pump; the pneumatic flow control is achieved with a two stage
control, by regulating its pressure and with a flow restrictor. Two Proportional Pressure
Regulators Valves, PRV1 and PRV2, are designed to make fine adjustments in pressure to
the air stream flowing from the manual pressure regulator to a flow restrictor. Two
automated flow restrictors, Proportional flow control Valves, PV1 and PV2, are designed
to make coarse adjustments in flow. Final flow control is achieved by Step changes in PV
orifice opening in combination with fine adjustments in the air flow stream pressure with
the PRV.
Adjustments in flow are based on the error difference between Calculated CT and Actual
CT. The proportional air pressure regulating valve, PRV1, and the exhaust pressure
regulating valve, PRV2, will be adjusted by the PLC based on the Error. The error will
cause pressures to be changed to affect the flow, positive or negative, respectively, to
and from the pump to match flow set point for the next cycle.
If the new value for PRV1 and/or PRV2 exceed their set pressure limits, (e.g. PRV1 0 to 30
psi, PRV2 0 to -14.5 psi), then, the respective PV1 and PV2 will adjust incrementally, (e.g. by
user defined increments (in the Basic Set Up Screen)), until the PRVs are back within
operational range.
6.3
Start-up
Assembly of the XCell
TM
ATF System is described in the following sections. This section
provides a startup guide for an XCell
TM
ATF System connected to a bioreactor and ready for
use, with the following general conditions:
•
An XCell
TM
ATF6 System is used in this example.
•
An XCell
TM
ATF System flow of 12L/min is required.
•
Bioreactor pressure is 0.0psi
•
∆
height between pump midpoint and vessel liquid level is 0cm
•
Regulated air pressure set to 35psi
