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Instrumenting scientiﬁc ideas

WORLD

PRECISION

INSTRUMENTS

PUL-1000

Microprocessor-controlled 4-Step

Micropipette Puller

Serial No._____________________

www.wpiinc.com

INSTRUCTION MANUAL

082318

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Summary of Contents for PUL-1000

Page 1: ...Instrumenting scienti c ideas WORLD PRECISION INSTRUMENTS PUL 1000 Microprocessor controlled 4 Step Micropipette Puller Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 082318...

Page 2: ......

Page 3: ...am Parameters 7 Glass Capillary Softening Test 8 MAINTENANCE 9 Replacing a Filament 9 Choosing a Filament 10 ACCESSORIES 10 SPECIFICATIONS 10 TROUBLESHOOTING 11 APPENDIX A EXAMPLE SEQUENCES 12 My Sequ...

Page 4: ...splay choose the correct sequence If you do not have a sequence yet choose one from the program storage NOTE Program sequence 00 is designed for OD 1 0mm borosilicate capillary glass WPI 1B100 4 and s...

Page 5: ...CTION PUL 1000 is a microprocessor controlled four stage horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording patch clamp microperfusion and microinjecti...

Page 6: ...le programs can be permanently be stored in memory for later recall The instrument contains two factory installed and tested programs Choose from the factory installed programs or create your own Cons...

Page 7: ...rior approval RMA required and instructions from WPI s Returns Department Goods returned unauthorized by collect freight may be refused If a return shipment is necessary use the original container if...

Page 8: ...ay shows the programming sequence and the defined parameters If you press the Stop key on the keypad you can toggle to the Glass Capillary Softening Test display Fig 3 The front panel has an intuitive...

Page 9: ...the right sequence to get the desired glass pipette requires experimentation The table below is provided to show some basic guidelines when setting up a sequence Parameter Increase Decrease Heat Longe...

Page 10: ...latinum iridium filament that provides enough heat to melt all common glasses except quartz After starting the program the filament begins to glow and heats the glass A solenoid gradually applies a mo...

Page 11: ...hrough the filament Values range from 0 to 999 The useful range of values depends upon the filament and glass type Usually the value used is close to the value determined in the Glass Capillary Soften...

Page 12: ...en You change the filament Lot numbers or capillary types change You create or modify a program 1 Press the STOP key to quit any running program The following window displays Fig 6 NOTE A force of 150...

Page 13: ...replace the filament 1 Loosen the set screws that secures the Filament Clamp on the Filament Support Block 2 Remove the old filament or its remaining pieces Be careful not to drop any mate rial into t...

Page 14: ...id penetration If you are using a box filament the size of the square box should be approximately 1 0mm to 1 5mm larger than the outside diameter of the glass to be pulled ACCESSORIES Part Number Desc...

Page 15: ...are not set properly for the properties of the new glass Run the Glass Softening Test to estab lish a new baseline heating parameter See Glass Capillary Softening Test on page 8 Install a new filamen...

Page 16: ...0 2 00 35 2 300 200 0 70 60 3 200 200 0 30 20 4 200 100 0 30 20 OD 1 14mm WPI 4878 STEP HEAT FORCE DISTANCE DELAY 1 700 350 3 00 55 2 600 100 1 00 60 3 400 200 0 30 25 4 350 120 0 30 20 My Sequences N...

Page 17: ...___________________________________ STEP HEAT FORCE DISTANCE DELAY 1 2 3 4 Name _____________________________________________ STEP HEAT FORCE DISTANCE DELAY 1 2 3 4 Name ______________________________...

Page 18: ...14 World Precision Instruments...

Page 19: ...special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or...

Page 20: ...D 61169 Friedberg Hesson Germany Tel 49 0 6031 1602171 Fax 49 0 6031 1602180 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 25e No8 Dongfang Rd Pudong District Shanghai 20...

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