Waters Xevo TQD, Operator'S, Overview And Maintenance Manual

Page: 58 / 250

3-6 Changing the Mode of Operation
APCI mode:
Hot gas from the IonSABRE II probe passes between the sample cone and the
corona pin, which is typically operated with a discharge current of 5 µA.
Mobile phase molecules rapidly react with ions generated by the corona
discharge to produce stable reagent ions. Analyte molecules introduced into
the mobile phase react with the reagent ions at atmospheric pressure and
typically become protonated (in the positive ion mode) or deprotonated (in the
negative ion mode). The sample and reagent ions then pass through the
sample cone and into the mass spectrometer.
Installing the IonSABRE II probe
Required materials
• Chemical-resistant, powder-free gloves
• Sharp knife or PEEK tubing cutter
To install the IonSABRE II probe:
Warning:
The LC system connections, IonSABRE II probe, and
source can be contaminated with biohazardous and/or toxic
materials. Always wear chemical-resistant, powder-free gloves
while performing this procedure.
Warning:
To avoid electric shock, ensure that the instrument is
prepared for working on the source before commencing this procedure.
IonSABRE II probe
Sample cone
Corona pin