3.5.2 Fitting a borosilicate glass capillary (nanovial)
Required materials:
• Chemical-resistant, powder-free gloves
• Needle-nose pliers
• Borosilicate glass capillary
• Fused silica syringe needle or GELoader tip
• Fused silica tubing cutter
To fit a borosilicate glass capillary (nanovial):
Warning:
To avoid lacerations, puncture injuries, and possible contamination with biohazardous
and toxic samples, do not touch the sharp end of the capillary.
Warning:
To avoid eye injury from broken fused silica lines, use eye protection when
performing this procedure.
Notice:
To avoid damaging capillaries, take great care when handling them; they are
extremely fragile. Always hold the blunt end, never the sharp end.
Warning:
To avoid electric shock, ensure that the NanoFlow stage is fully retracted
from the source before beginning this procedure.
Warning:
To avoid personal contamination with biohazards, toxic materials, and corrosive
materials, wear chemical-resistant gloves when performing this procedure.
1.
Loosen the stage retaining screw.
2.
Pull the stop screw to release the stage.
3.
Slide the stage out of the NanoFlow source enclosure, and remove the magnetic cover.
4.
Unscrew the retaining screw, and lift the sprayer from the stage.
5.
Unscrew the union from the end of the sprayer assembly.
June 9, 2016, 715004990 Rev. A
Page 80
