RADAR is an additional mode with which you simultaneously collect data from the MRM and full
scan MS modes. RADAR mode can also acquire all detectable ions in both positive and negative
full scan MS.
1.7.1 Product (daughter) ion mode
Product ion mode is the most commonly used MS/MS operating mode. You can specify an ion of
interest for fragmentation in the collision cell, thus yielding structural information.
Figure 1–4: Product ion mode:
MS1 - Static (at precursor mass)
Collision cell - Fragment precursor ions and pass all masses
MS2 - Scanning
1.7.1.1 Typical applications
You typically use product ion mode for the following applications:
• Method development for MRM screening studies:
• Identifying product ions for use in MRM transitions.
• Optimizing CID tuning conditions to maximize the yield of a specific product ion to be used
in MRM analysis.
• Structural elucidation (for example, peptide sequencing).
1.7.2 Precursor (parent) ion mode
Figure 1–5: Precursor ion mode:
June 9, 2016, 715004990 Rev. A
Page 31
