Waters Xevo TQ MS Operator'S, Overview And Maintenance Manual Download Page 1

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Waters Xevo TQ MS

Operator’s Overview and Maintenance Guide

71500130002 / Revision D

Summary of Contents for Xevo TQ MS

Page 2: ...ii February 11 2013 71500130002 Rev D...

Page 3: ...ing from its use For the most recent revision of this document consult the Waters Web site waters com Trademarks ACQUITY ACQUITY UPLC Connections INSIGHT ESCi MassLynx nanoACQUITY UPLC UltraPerformanc...

Page 4: ...ters com Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use transportation removal or disposal of any Waters product You can reach us via the Internet...

Page 5: ...ressure remains above 690 kPa 6 9 bar 100 psi You must also install a gas fail device that interrupts the solvent flowing from the LC system in the event the supply of nitrogen fails Warning To confir...

Page 6: ...Rev D High temperature hazard Xevo TQ MS high temperature hazard Warning To avoid burn injuries avoid touching the source ion block assembly when operating or servicing the instrument TP03127 TQ MS T...

Page 7: ...es the extent of decontamination required and the level of residual contamination permissible That authority must also prescribe the method of decontamination to be used and the appropriate protection...

Page 8: ...issions notice This class A digital product apparatus complies with Canadian ICES 003 Cet appareil num rique de la classe A est conforme la norme NMB 003 Electrical power safety notice Do not position...

Page 9: ...strument and explains how to prepare it change its modes of operation and maintain it Symbol Definition Manufacturer Authorized representative of the European Community Confirms that a manufactured pr...

Page 10: ...that represent subnormal normal and above normal levels of a compound Ensure that QC sample results fall within an acceptable range and evaluate precision from day to day and run to run Data collecte...

Page 11: ...erated and or used conductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class A products are suitable for use in commercial that is nonresidentia...

Page 12: ...xii February 11 2013 71500130002 Rev D...

Page 13: ...otice viii Safety hazard symbol notice viii Equipment misuse notice viii Safety advisories viii Operating this instrument ix Applicable symbols ix Audience and purpose ix Intended use of the Xevo TQ M...

Page 14: ...modes 32 MS MS operating modes 33 Product daughter ion mode 34 Precursor parent ion mode 34 Multiple reaction monitoring mode 35 Constant neutral loss mode 36 ScanWave daughter scan mode 37 Leak senso...

Page 15: ...APCI mode 57 Installing the APCI probe 58 Removing the APCI probe 60 Combined APPI APCI source 61 APPI operation 61 APCI operation 62 Dual mode operation 63 The combined APPI APCI source components 6...

Page 16: ...om the source 93 Operating the source isolation valve 94 Removing O rings and seals 97 Cleaning the instrument case 98 Emptying the nitrogen exhaust trap bottle 98 Inspecting the Varian roughing pump...

Page 17: ...sembly 140 Removing the ion block assembly ion block support and hexapole from the source assembly 140 Cleaning the hexapole assembly 142 Fitting the hexapole assembly PEEK ion block support and ion b...

Page 18: ...als 185 Fitting the new APPI lamp drive assembly O rings 191 A Safety Advisories 195 Warning symbols 196 Specific warnings 197 Caution advisory 200 Warnings that apply to all Waters instruments and de...

Page 19: ...cting to the collision cell gas supply 230 Connecting the nitrogen exhaust line 231 Connecting the liquid waste line 234 Connecting the workstation 236 Connecting Ethernet cables 237 Input output sign...

Page 20: ...xx February 11 2013 71500130002 Rev D...

Page 21: ...instrument including its controls and connections for gas and plumbing Contents Topic Page Waters Xevo TQ MS 22 Ionization techniques and source probes 27 IntelliStart Fluidics system 29 Ion optics 31...

Page 22: ...rmance ZSpray dual orthogonal API sources Standard multi mode electrospray ionization atmospheric pressure chemical ionization combined electrospray ionization and atmospheric pressure chemical ioniza...

Page 23: ...tart Fluidics system is built into the mass spectrometer It delivers sample directly to the MS probe from the LC column or from three integral reservoirs The integral reservoirs can also deliver sampl...

Page 24: ...manager sample manager column heater optional sample organizer optional detectors a specialized ACQUITY UPLC column and software to control the system For further instruction see the ACQUITY UPLC Syst...

Page 25: ...om 75 m to 1 mm the flow rates can extend to 100 L min The column hardware and the matched outlet tubing can withstand pressure of as much as 69 000 kPa 690 bar 10 000 psi The column dimensions allow...

Page 26: ...analyzes manages and distributes mass spectrometry ultraviolet UV evaporative light scattering and analog data OpenLynx and TargetLynx application managers are included as standard software with Mass...

Page 27: ...accommodate eluent flow rates of up to 2 mL min Combined electrospray ionization and atmospheric pressure chemical ionization ESCi Combined electrospray ionization and atmospheric pressure chemical i...

Page 28: ...le concentration the ion currents observed approximate those seen in normal flow rate electrospray However for similar experiments NanoFlow s significant reduction in sample consumption accompanies si...

Page 29: ...rvoir bottles 30 mL for instrument setup and calibration Use low volume vials 1 5 mL to infuse smaller volumes See Installing the reservoir bottles on page 47 Tip The reservoirs can also deliver sampl...

Page 30: ...ee reservoirs or extinguish the LEDs for light sensitive samples Recommendation Use reservoir A for the calibrant solution reservoir B for tuning compounds and reservoir C for analyte optimization sol...

Page 31: ...cording to their mass to charge ratio m z 4 The mass separated ions pass into the collision cell T Wave ScanWave where they undergo collision induced dissociation CID or pass to the second quadrupole...

Page 32: ...selected ion recording SIR mode for quantitation when you cannot find a suitable fragment ion to perform a more specific multiple reaction monitoring MRM analysis See MS MS operating modes on page 33...

Page 33: ...perating modes Operating mode MS1 Collision cell MS2 Product daughter ion spectrum Static at precursor mass Pass all masses Scanning Precursor parent ion spectrum Scanning Static at product mass MRM S...

Page 34: ...typically use product ion mode for the following applications Method development for MRM screening studies Identifying product ions for use in MRM transitions Optimizing CID tuning conditions to maxi...

Page 35: ...pared with scanning mode MS MS This mode is the most commonly used acquisition mode for quantitative analysis allowing the compound of interest to be isolated from the chemical background noise Multip...

Page 36: ...of a certain mass If it does the loss registers at the detector In constant neutral loss mode the spectrum shows the masses of all precursors that actually lost a fragment of a certain mass Constant n...

Page 37: ...ion However in this ScanWave mode the cell accumulates ions intact or fragments and then releases them according to their mass in synchrony with the second quadrupole mass analyzer This mode of operat...

Page 38: ...ctions for complete details Vacuum system An external roughing rotary vane pump a dry pump option is available and two internal turbomolecular pumps create the source vacuum The turbomolecular pumps e...

Page 39: ...onnections 1 10 1 LA N EPC Com Port Video Output Service Bus 1 2 3 4 5 6 7 8 9 10 Event In 1 ExternalConnections1 Not used CE Interlock OUT Event In 2 Not used IN IN Com N C N O Com N C N O 1 2 3 4 5...

Page 40: ...1 Waters Xevo TQ MS Overview 40 February 11 2013 71500130002 Rev D...

Page 41: ...er describes how to start and shut down the mass spectrometer Contents Topic Page Starting the mass spectrometer 42 Preparing the IntelliStart Fluidics system 47 Rebooting the mass spectrometer 49 Lea...

Page 42: ...sure the nitrogen supply is connected to the instrument s nitrogen inlet connection see the figure on page 39 Requirement The nitrogen must be dry and oil free with a purity of at least 95 Regulate th...

Page 43: ...nager s flow LED the sample manager s run LED and the mass spectrometer s Operate LED remain off 6 Start the MassLynx or UNIFI software and monitor the Instrument Console software for messages and LED...

Page 44: ...mp Tip After a 20 second delay during which the turbopump is starting the roughing pump starts The Instrument System pane shows that the instrument is in Standby mode and the Operate LED remains off c...

Page 45: ...r LED The power LED to the top left hand side of the mass spectrometer s front panel indicates when the mass spectrometer is powered on or powered off Operate LED The Operate LED on the right hand sid...

Page 46: ...UITY Xevo TQ MS system at appropriate gas flows and desolvation temperatures IntelliStart software automatically sets these parameters when you enter a flow rate according to the following table Flow...

Page 47: ...aller volumes The low volume vials have a volume of 1 5 mL Required materials Chemical resistant powder free gloves To install the reservoir bottles 1 Remove the reservoir bottle caps 2 Screw the rese...

Page 48: ...nfusion pump Whenever you replace a solution bottle purge the infusion pump with the solution that you are going to use next See the mass spectrometer s online Help for details Tip Depending on the so...

Page 49: ...of these conditions applies The mass spectrometer software fails to initialize Immediately following a software upgrade To reboot the mass spectrometer 1 Open the mass spectrometer s front left hand d...

Page 50: ...put the mass spectrometer in Standby mode see the online Help for details Emergency shutdown of the mass spectrometer To shut down the mass spectrometer in an emergency 1 Operate the power button on...

Page 51: ...pressure chemical ionization APCI atmospheric pressure chemical ionization Combined Atmospheric Pressure Photoionization APPI APCI NanoFlow ESI Note Available source options can vary depending on the...

Page 52: ...ent for working on the source on page 85 2 Remove the protective sleeve if fitted from the ESI probe tip Warning To avoid personal contamination with biohazards or toxic materials and to avoid spreadi...

Page 53: ...l facing you carefully slide the ESI probe into the hole in the probe adjuster assembly Requirement The probe location dowel must align with the location hole of the probe adjuster assembly TP03129 Lo...

Page 54: ...eak broadening use 0 004 inch ID tubing for sample flow rates 1 2 mL min use 0 005 inch ID tubing for sample flow rates 1 2 mL min Requirement If you are replacing the tubing supplied with the instrum...

Page 55: ...ection between the diverter valve and the ESI probe Tip The other plumbing connections are omitted for clarity 8 Close the access door Caution To prevent damage to the tubing ensure that it does not b...

Page 56: ...5 Carefully remove the ESI probe from the probe adjuster assembly 6 If available fit the protective sleeve to the ESI probe tip Warning To avoid personal contamination with biohazards or toxic materi...

Page 57: ...he APCI interface consists of the ESI APCI ESCi enclosure fitted with a corona pin and an APCI probe Mobile phase from the LC column enters the probe where it is pneumatically converted to an aerosol...

Page 58: ...juster assembly ensuring that the probe location dowel aligns with the probe adjuster assembly location hole Warning To avoid personal contamination with biohazards or toxic materials and to avoid spr...

Page 59: ...use 0 005 inch ID tubing for sample flow rates 1 2 mL min Requirement If you are replacing the tubing supplied with the instrument minimize the length of the tube connecting the diverter valve to the...

Page 60: ...3 Disconnect the diverter valve tubing from the APCI probe 4 Unscrew the probe locking ring 5 Carefully remove the probe from the probe adjuster assembly Warning To avoid personal contamination with b...

Page 61: ...mbly is advanced into the source APPI mode The APCI probe introduces vaporized sample into the source where photons generated by an ultra violet UV lamp mounted in the APPI lamp drive assembly produce...

Page 62: ...introduces vaporized sample into the source The sample passes between the sample cone and the corona pin which typically operates with a discharge current of 5 A The corona discharge generates ions t...

Page 63: ...ource for dual operation When the source is configured for dual operation in APCI mode current is applied to the corona pin but the repeller electrode is inactive Dual operation in APCI mode When the...

Page 64: ...ed APPI APCI source enclosure Switching on the UV lamp from the MassLynx Tune window provides a constant photon output You vary the intensity of incident radiation upon the sample molecules by adjusti...

Page 65: ...ined APPI APCI source February 11 2013 71500130002 Rev D 65 APPI lamp drive assembly inside the source enclosure TP03201 APPI lamp drive assembly Source enclosure UV lamp and repeller electrode APCI p...

Page 66: ...robe see Removing the ESI probe on page 56 If you are removing an APCI probe see Removing the APCI probe on page 60 3 Remove the existing source enclosure see Removing the source enclosure from the in...

Page 67: ...ccess door 9 Install the APCI probe to the source and ensure that it is working correctly see Installing the APCI probe on page 58 Tip An automatic pressure test is performed each time the source encl...

Page 68: ...l 5 Remove the source enclosure see Removing the source enclosure from the instrument on page 87 6 Remove the corona pin see Removing the corona pin from the source on page 93 7 Fit the blanking plug...

Page 69: ...Z stage three axis manipulator which slides on a pair of guide rails that allow its withdrawal from the source enclosure for maintenance and changes A light within the source provides illumination fo...

Page 70: ...moving the source enclosure from the instrument on page 87 Warning To avoid personal contamination with biohazards or toxic materials and to avoid spreading contamination to uncontaminated surfaces we...

Page 71: ...tput mounted beneath the stage on the front of the NanoFlow source and your sprayer Tip For details regarding how to fit each sprayer see the corresponding reference Waters Universal NanoFlow Sprayer...

Page 72: ...ion Note The NanoFlow stage contains a high voltage interlock so that unless the sprayer is pushed fully forward in the source the capillary voltage the voltage applied to the sprayer assembly and the...

Page 73: ...and remove the magnetic cover 4 Unscrew the retaining screw and lift the sprayer from the stage Warning To avoid lacerations puncture injuries and possible contamination with biohazardous and toxic sa...

Page 74: ...illary from the sprayer 7 Carefully remove the new borosilicate glass capillary from its case by lifting vertically while pressing down on the foam with two fingers 8 Load sample into the capillary us...

Page 75: ...of conductive elastomer over the blunt end of the capillary 10 Fit the capillary into the holder probe 11 Finger tighten the nut so that 5 mm of glass capillary is protruding from its end Tip This dis...

Page 76: ...ES Source tab is set to 0 15 Carefully push the stage back into the NanoFlow source enclosure using the stop and handle Caution To avoid damage to the capillary tip adjust the sprayer tip position be...

Page 77: ...2 mm Capillary tip position For tuning instructions see the MassLynx Xevo TQ online help Tuning manually for NanoFlow operation Restarting a stalled borosilicate glass capillary electrospray Should t...

Page 78: ...3 Changing the Mode of Operation 78 February 11 2013 71500130002 Rev D...

Page 79: ...ing and refitting the source enclosure 87 Installing and removing the corona pin 90 Operating the source isolation valve 94 Removing O rings and seals 97 Cleaning the instrument case 98 Emptying the n...

Page 80: ...eplacing the corona pin 165 Replacing the APCI probe heater 166 Replacing the ion block source heater 170 Replacing the source assembly seals 174 Replacing the air filter 179 APPI APCI source changing...

Page 81: ...t filter Annually See page 104 Clean the source components When they are visibly fouled the background or high peak contaminants are unacceptably high or sensitivity decreases to unacceptable levels S...

Page 82: ...acuated See page 166 Replace the ion block heater cartridge If the heater fails to heat when the instrument is pumped down evacuated See page 170 Replace the source assembly seals Annually See page 17...

Page 83: ...quest to Waters customer support As an option Remote Desktop is a real time collaboration tool a service that controls two way connection with the ACQUITY UPLC system by enabling the Connections INSIG...

Page 84: ...sed see the Material Safety Data Sheets for the solvents in use Warning To avoid electric shock do not remove the instrument s panels There are no user serviceable items inside the instrument ensure t...

Page 85: ...are To use MassLynx software to prepare the instrument for working on the source 1 In the Instrument Console click Stop Flow to stop the LC flow or Note If column flow is required divert the LC flow t...

Page 86: ...column flow is required divert the LC flow to waste a On the Setup pane click Fluidics b On the Sample Fluidics page from the Flow Path menu select Waste 2 Click Instrument Standby Mode and confirm th...

Page 87: ...page 60 3 Slide open the instrument s source interface door see the figure on page 24 4 Disconnect the probe adjuster and options cables from the instrument s connectors 5 Pull the source enclosure re...

Page 88: ...ocedures 88 February 11 2013 71500130002 Rev D 6 Using both hands grasp the source enclosure and lift it vertically off the two supporting studs on the source adaptor housing TP03164 Supporting stud S...

Page 89: ...ectors 4 Slide closed the instrument s source interface door Warning To avoid personal contamination with biohazards or toxic materials and to avoid spreading contamination to uncontaminated surfaces...

Page 90: ...osure Warning To avoid personal contamination with biohazards or toxic materials and to avoid spreading contamination to uncontaminated surfaces wear clean chemical resistant powder free gloves while...

Page 91: ...rona pin mounting contact 4 Fit the corona pin to the mounting contact ensuring that the corona pin is securely mounted and that its tip aligns with the sample cone aperture Corona pin 5 Close the sou...

Page 92: ...3 71500130002 Rev D 6 Look through the source window and use the vernier probe adjuster to position the ESI probe tip so that it is pointing approximately midway between the tips of the sample cone an...

Page 93: ...ting contact see the figure on page 91 5 Close the source enclosure Warning To avoid personal contamination with biohazards or toxic materials and to avoid spreading contamination to uncontaminated su...

Page 94: ...losure Warning To avoid personal contamination with biohazards or toxic materials and to avoid spreading contamination to uncontaminated surfaces wear clean chemical resistant powder free gloves while...

Page 95: ...ng the source isolation valve February 11 2013 71500130002 Rev D 95 3 Close the source isolation valve by moving its handle counterclockwise to the vertical position Isolation valve handle in closed p...

Page 96: ...on with biohazards or toxic materials and to avoid spreading contamination to uncontaminated surfaces wear clean chemical resistant powder free gloves while performing this procedure The source compon...

Page 97: ...f tool 1 to impale the O ring or seal 2 Pull the O ring or seal from its groove if necessary use tool 2 as an aid 3 Dispose of the O ring or seal in accordance with local environmental regulations Cau...

Page 98: ...haust trap bottle Inspect the nitrogen exhaust trap bottle in the instrument s exhaust line daily and empty it before it is more than approximately 10 full Nitrogen exhaust trap bottle Caution To avoi...

Page 99: ...cap 6 Secure the nitrogen exhaust trap bottle in the upright position 7 Close the source enclosure Tip An automatic leak test will now be performed Software Action MassLynx In the instrument console...

Page 100: ...100 February 11 2013 71500130002 Rev D 8 To start the LC flow follow the action below for your software Software Action MassLynx In the instrument console click Start Flow UNIFI On the System Console...

Page 101: ...lass Inspect the oil level at weekly intervals with the pump running the oil level is typically at approximately 30 to 60 of the maximum oil level This corresponds to approximately the maximum oil lev...

Page 102: ...he roughing pump oil level is found to be low you must add oil to the roughing pump See Inspecting the Varian roughing pump oil level on page 101 Varian roughing pump Required materials Chemical resis...

Page 103: ...e Starting the mass spectrometer on page 42 Tips After you add oil to the pump the following situations can occur The oil level drops slightly during the first month of operation The oil changes color...

Page 104: ...own the mass spectrometer see the mass spectrometer s online Help for details 2 Allow the roughing pump to cool 3 Place the container for capturing the used oil under the pump s drain plug see the fig...

Page 105: ...y into the container 7 Dispose of the roughing pump oil in accordance with local environmental regulations 8 Use the lint free cloth to remove loose particles from the magnetic tip of the oil drain pl...

Page 106: ...g the oil mist canister cover to the oil mist canister 2 Remove the oil mist canister cover from the oil mist canister Tip The cover can be tight and difficult to remove Warning To avoid personal cont...

Page 107: ...ing on the oil mist canister cover is correctly seated 7 Center the spring on top of the oil mist filter 8 Fit the spring over the collar on the oil mist canister cover and then fit the cover to the o...

Page 108: ...ug seal it only makes the plug difficult to remove later Important To maintain pump performance use Varian GP45 oil 3 Using the funnel pour 1 L of Varian GP45 oil into the oil filler aperture Tip The...

Page 109: ...slightly during the first month of operation The oil changes color darkens over time After running the pump for 12 to 48 hours a few drops of oil sometimes appear near the filler plug Excess oil aroun...

Page 110: ...g cone assembly on page 111 If cleaning the sample cone and cone gas nozzles fails to increase signal sensitivity also clean the extraction cone See Cleaning the extraction cone on page 120 If cleanin...

Page 111: ...and to avoid spreading contamination to uncontaminated surfaces wear clean chemical resistant powder free gloves when performing this procedure The source components can be contaminated Warning To avo...

Page 112: ...gas nozzle handle and use it to rotate the sampling cone assembly 90 degrees moving the handle from the vertical to the horizontal position 3 Slide the sampling cone assembly out of the ion block asse...

Page 113: ...l To disassemble the sampling cone assembly 1 On the combined 2 5 mm Allen wrench and cone extraction tool slide the collar to the end of the tool 2 Insert the collar in the sample cone Warning To avo...

Page 114: ...m the sample cone 5 If the O ring shows signs of deterioration or damage dispose of it in accordance with local environmental regulations 6 Unscrew and remove the PEEK cone gas nozzle handle from the...

Page 115: ...s nozzle 1 If the sample cone contains debris place a drop of formic acid on its aperture 2 Immerse the sample cone cone gas nozzle and cone gas nozzle handle in separate glass vessels containing 1 1...

Page 116: ...n the ultrasonic bath for 10 minutes 5 Carefully remove the components from the vessels and blow dry them with inert oil free gas 6 Inspect each component for persisting contamination If contamination...

Page 117: ...ten 2 Carefully fit the sample cone into the cone gas nozzle 3 Fit the O ring into the groove created between the sample cone and cone gas nozzle Fit a new O ring if the old one has been disposed of C...

Page 118: ...ated surfaces wear clean chemical resistant powder free gloves when working with the source components Warning To avoid puncture wounds take great care while working with the source enclosure open if...

Page 119: ...so that the cone gas nozzle handle is oriented horizontally and at the top and then slide the sampling cone assembly into the ion block assembly 3 Grasp the cone gas nozzle handle and use it to rotate...

Page 120: ...er see the mass spectrometer s online Help for details 2 Close the source isolation valve see To close the source isolation valve before starting a maintenance procedure on page 94 Warning To avoid pe...

Page 121: ...the combined 2 5 mm Allen wrench and cone extraction tool to unscrew the 4 captive ion block assembly securing screws 4 Remove the ion block assembly from the PEEK ion block support TP03130 Ion block...

Page 122: ...Allen wrench and cone extraction tool to loosen the captive screws securing the 2 PEEK extraction cone retainer clips and then rotate the retainer clips clear of the extraction cone Warning To avoid p...

Page 123: ...he extraction cone handle insulator from the extraction cone handle Caution To avoid damaging the extraction cone aperture take great care when removing the extraction cone from the ion block To avoid...

Page 124: ...r To clean the extraction cone 1 Immerse the extraction cone in the glass vessel containing 1 1 methanol water Tip If the extraction cone is obviously contaminated use 45 45 10 methanol water formic a...

Page 125: ...low dry it using inert oil free gas 5 Inspect the extraction cone for persisting contamination If contamination is present do as follows a Use the wash bottle containing 1 1 methanol water to rinse th...

Page 126: ...o the source assembly Required materials Chemical resistant powder free gloves Combined 2 5 mm Allen wrench and cone extraction tool Warning To avoid personal contamination with biohazards or toxic ma...

Page 127: ...d 2 5 mm Allen wrench and cone extraction tool to fit and then slowly tighten the 4 ion block assembly securing screws sequentially and in small increments 3 Open the source isolation valve see Operat...

Page 128: ...block assembly if cleaning the sample cone cone gas nozzle and extraction cone fails to increase signal sensitivity Disassembling the source ion block assembly Required materials Chemical resistant p...

Page 129: ...mpling cone assembly out of the ion block assembly Warning To avoid personal contamination with biohazards or toxic materials and to avoid spreading contamination to uncontaminated surfaces wear clean...

Page 130: ...5 mm Allen wrench and cone extraction tool to loosen the 2 ion block cover plate captive securing screws 6 Remove the ion block cover plate 7 Grasp the isolation valve and pull it out of the ion bloc...

Page 131: ...wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw 11 Use the needle nose pliers to grasp the PEEK terminal block and partially lift it out of the ion block Warn...

Page 132: ...heat shrink tubing on the heater cartridge assembly and carefully slide it and the PEEK terminal block out of the ion block 13 Use the O ring removal kit to carefully remove the cover seal from the i...

Page 133: ...rew and remove the ion block blanking plug and associated seal 17 If the blanking plug seal shows signs of deterioration or damage dispose of it in accordance with local environmental regulations Warn...

Page 134: ...raction cone retainer clips 19 Remove the extraction cone from the ion block Caution To avoid damaging the extraction cone aperture take great care when removing the extraction cone from the ion block...

Page 135: ...ion block assembly February 11 2013 71500130002 Rev D 135 20 Remove the extraction cone handle insulator from the extraction cone handle 21 Remove the extraction cone seal from the ion block Extracti...

Page 136: ...ade or better 1 1 methanol water Large beaker To clean the ion block components 1 Immerse the ion block and isolation valve in separate glass vessels containing 1 1 methanol water Tip If the component...

Page 137: ...vessels and blow dry them using inert oil free gas 5 Inspect each component for persisting contamination If contamination is present do as follows a Use the wash bottle containing 1 1 methanol water t...

Page 138: ...e extraction tool to tighten the captive screw securing each extraction cone retainer clip to the ion block 6 Fit the blanking plug seal to the ion block blanking plug Fit a new seal if the old one ha...

Page 139: ...ld isolation valve O ring has been disposed of soak a new O ring in isopropyl alcohol for a few minutes Rationale Doing so lubricates the O ring and aids your fitting the O ring to the isolation valve...

Page 140: ...aterials Chemical resistant powder free gloves 3 mm Allen wrench O ring removal kit To remove the ion block assembly ion block support and hexapole 1 Remove the ion block assembly from the PEEK ion bl...

Page 141: ...show signs of deterioration or damage dispose of them in accordance with local environmental regulations 6 Carefully grasp the source hexapole assembly and remove it from the adaptor housing Warning T...

Page 142: ...containing HPLC grade or better 1 1 methanol water Large beaker Small flat blade screwdriver To clean the source hexapole assembly 1 Bend the PEEK or Teflon tube into a hook shape 2 Insert one end of...

Page 143: ...gas 7 Inspect the hexapole assembly for persisting contamination If contamination is present do as follows a Use the wash bottle containing methanol to rinse the source hexapole assembly over the larg...

Page 144: ...mbly fully into place 2 Ensure that the grooves for the PEEK ion block support O rings are free from dirt and debris Tip If contamination is present use 1 1 methanol water applied to a lint free cloth...

Page 145: ...securing screws Tip Look through the PEEK ion block support as you tighten the securing screws Ensure that the hexapole springs do not buckle and touch a hexapole rod 6 Fit the ion block assembly to t...

Page 146: ...ench To remove the ESI probe tip and gasket 1 Remove the ESI probe from the source see Removing the ESI probe on page 56 Warning To avoid personal contamination with biohazards or compounds that are t...

Page 147: ...ruary 11 2013 71500130002 Rev D 147 2 Use the 10 mm wrench to remove the probe tip Tip If the probe tip is difficult to remove use the 7 mm wrench in conjunction with the 10 mm wrench Probe tip 10 mm...

Page 148: ...ccordance with local environmental regulations 5 If the probe tip is damaged dispose of it in accordance with local environmental regulations Warning To avoid spreading contamination dispose of the pr...

Page 149: ...h to tighten the probe tip 4 Use the nebulizer adjuster knob to adjust the capillary so that it protrudes by approximately 0 5 mm from the end of the probe 5 Fit the ESI probe to the source see Instal...

Page 150: ...8 mm wrench 10 mm wrench Needle nose pliers To remove the existing capillary 1 Remove the probe from the source see Removing the ESI probe on page 56 Warning To avoid personal contamination with bioha...

Page 151: ...151 2 Use the combined 2 5 mm Allen wrench and cone extraction tool to remove the 3 probe end cover retaining screws 3 Remove the end cover from the probe assembly 4 Unscrew and remove the nebulizer...

Page 152: ...ry 11 2013 71500130002 Rev D 5 Use the 10 mm wrench to remove the probe tip Tip If the probe tip is difficult to remove use the 7 mm wrench in conjunction with the 10 mm wrench Probe tip 10 mm wrench...

Page 153: ...Remove the PEEK union UNF coupling assembly and capillary from the probe 8 Unscrew and remove the knurled collar from the UNF coupling 9 Remove the knurled collar and conductive sleeve from the capil...

Page 154: ...er tight only 12 Remove the ferrule and PTFE liner sleeve from the capillary 13 Remove the capillary from the UNF coupling 14 Dispose of the capillary PTFE liner sleeve and ferrule in accordance with...

Page 155: ...oximately 60 cm 24 inches piece of red PEEK tubing squarely that is perpendicular to its horizontal axis 2 Insert one end of the red PEEK tubing in the probe inlet connector and screw the connector fi...

Page 156: ...be twisted 9 Slide a new conductive sleeve and the knurled collar over the capillary 10 Tighten the knurled collar to the UNF coupling 11 Perform a leak test by attaching the free end of the PEEK tubi...

Page 157: ...the nebulizer adjuster knob onto the probe assembly 18 Fit the end cover to the probe assembly 19 Use the combined 2 5 mm Allen wrench and cone extraction tool to fit and tighten the 3 probe end cover...

Page 158: ...further details To clean the APCI probe tip 1 On the Instrument Console system tree click Xevo TQ MS Manual optimization 2 On the manual optimization page click to stop the liquid flow 3 Click Gas to...

Page 159: ...tool To remove the existing capillary 1 Remove the probe from the source see Removing the APCI probe on page 60 2 Use the combined 2 5 mm Allen wrench and cone extraction tool to remove the 3 probe en...

Page 160: ...ve the end cover 4 Unscrew and remove the nebulizer adjuster knob 5 Remove the PEEK union UNF coupling assembly and capillary from the probe 6 Use the 7 mm wrench to loosen the locknut End cover Nebul...

Page 161: ...e ferrule from the capillary 9 Remove the capillary from the UNF coupling 10 Dispose of the capillary and ferrule in accordance with local environmental regulations Warning To avoid spreading contamin...

Page 162: ...cut an approximately 60 cm 24 inches piece of red PEEK tubing squarely that is perpendicular to its horizontal axis 2 Insert one end of the red PEEK tubing in the probe inlet connector and screw the...

Page 163: ...ing the free end of the PEEK tubing to an LC pump and pumping 50 50 acetonitrile water through it at 1 mL min If leakage occurs disassemble and remake the connection and repeat the leak test If the ba...

Page 164: ...probe assembly 18 Fit the probe end cover to the probe assembly 19 Use the combined 2 5 mm Allen wrench and cone extraction tool to fit and tighten the 3 end cover securing screws 20 Fit the probe hea...

Page 165: ...e it clean with a methanol saturated tissue 3 Install the corona pin in the source see Installing the corona pin in the source on page 90 Warning To avoid personal contamination with biologically haza...

Page 166: ...CI probe heater 1 Remove the probe from the source see Removing the APCI probe on page 60 2 Use the jeweler s screwdriver to loosen the 2 set screws securing the probe heater cover to the probe Warnin...

Page 167: ...Rev D 167 3 Carefully pull the probe heater cover off the probe Caution To avoid damaging the probe heater s electrical wiring take great care when removing the probe heater cover or while the probe h...

Page 168: ...tion To avoid damaging the probe heater s electrical wiring take great care when gripping and handling the heater To avoid damaging the probe heater s electrical connections do not twist the heater wh...

Page 169: ...obe assembly 3 Fit the probe heater to the probe assembly ensuring that the heater is fully seated on the probe assembly 4 Fit the probe heater cover to the probe assembly Caution To avoid damaging th...

Page 170: ...the instrument is pumped down evacuated Required materials Chemical resistant powder free gloves Needle nose pliers Combined 2 5 mm Allen wrench and cone extraction tool Ion block source heater To re...

Page 171: ...e isolation valve is closed 3 Use the combined 2 5 mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate 4 Remove the ion block cover plate Isolati...

Page 172: ...m Allen wrench and cone extraction tool to remove the 2 screws securing the heater wires to the PEEK terminal block 6 Use the needle nose pliers to carefully swing the ring terminal tags out of the te...

Page 173: ...m Allen wrench and cone extraction tool to fit and tighten the 2 screws securing the heater wires to the PEEK terminal block 12 Fit the ion block cover plate to the ion block assembly and then use the...

Page 174: ...bly nebulization gas seal Source enclosure seal Source enclosure nebulizer gas seal Source enclosure desolvation gas seal Source enclosure pressure test gas seal Removing the probe adjuster assembly p...

Page 175: ...removal kit to carefully remove the following seals from the probe adjuster assembly Probe seal Nebulizer gas seal See Removing O rings and seals on page 97 Warning To avoid personal contamination wi...

Page 176: ...gas seal Desolvation gas seal Auxiliary gas seal 4 Dispose of all the seals in accordance with local environmental regulations Warning To avoid spreading contamination dispose of all the seals accordi...

Page 177: ...clean the grooves Requirement Ensure that the tails of the source enclosure seals are correctly located in the groove when fitting them to the source enclosure 2 Fit the new source enclosure seal to t...

Page 178: ...e seals have a special cross section fit them in the groove as shown 4 Fit the following new seals to the probe adjuster assembly Probe seal Nebulizer gas seal 5 Refit the source enclosure to the inst...

Page 179: ...Replacing the air filter You must replace the air filter annually Required materials Needle nose pliers New filter To replace the air filter 1 Open the access door to the fluidics pump 2 Unscrew the...

Page 180: ...vertically from the its slot in the instrument Tip If necessary use the needle nose files to grasp the filter 5 Dispose of the filter 6 Fit the new filter into the instrument 7 Fit the filter cover t...

Page 181: ...nclosure 3 Retrieve the 2 5 mm Allen wrench from its storage recess at the top right hand side of the source enclosure open Warning To avoid personal contamination with biologically hazardous toxic or...

Page 182: ...ook the short end of the Allen wrench through the ring on the back of the bulb extraction plug and tug to remove it 5 Insert the length of 4 mm nylon tube through the back of the lamp drive assembly a...

Page 183: ...mbly Tip The lamp glass is magnesium fluoride Avoid touching it because dirt or other contaminants on the window significantly reduce UV transmission 8 Refit the lamp bulb access plug 9 Close the sour...

Page 184: ...1 Prepare the instrument for working on the source see Preparing the instrument for working on the source on page 85 2 Pull the source enclosure release located at the bottom right hand side outwards...

Page 185: ...rument starts Removing the APPI lamp drive assembly seals Required materials Chemical resistant powder free gloves 2 5 mm Allen wrench from storage recess inside the source door Small Phillips cross h...

Page 186: ...the source enclosure on its face Warning To avoid personal contamination with biologically hazardous toxic or corrosive materials and to avoid spreading contamination to uncontaminated surfaces wear...

Page 187: ...6 Remove the remaining 2 screws which are hidden by the release handle 7 Slide the cover off the lamp drive 8 Remove the 4 source enclosure cover screws 9 Ease the source enclosure cover over the lamp...

Page 188: ...the lamp assembly shaft and flange out of the APPI source enclosure Tip The cables remain attached to the shaft which you fully withdraw and lay on the bench beside the source enclosure TP03205 Mounti...

Page 189: ...ting shaft 15 Slide the shaft mounting flange all the way off the shaft taking care to note the correct orientation for reassembly 16 Use the O ring removal kit to carefully remove the lamp bulb seali...

Page 190: ...enclosure side see Removing O rings and seals on page 97 19 Dispose of the O rings in accordance with local environmental regulations Warning To avoid spreading contamination ensure that the O rings a...

Page 191: ...e groove Then use a suitable tool one with a circular cross section to roll the remainder of the O ring into the groove 2 Fit the lamp bulb sealing O ring in the lamp aperture 3 Fit the two new O ring...

Page 192: ...Doing so ensures that the lamp mounting flange is uniformly seated on the APPI source enclosure side plate 8 Use the 2 5 mm Allen wrench to tighten the four mounting flange securing screws 9 Remove t...

Page 193: ...mounting shaft from the source enclosure 16 Refit the lamp assembly collar cover and secure it on its base with the 4 screws 17 Refit the lamp assembly cover and secure it on its base 2 screws and ab...

Page 194: ...4 Maintenance Procedures 194 February 11 2013 71500130002 Rev D...

Page 195: ...ar in product manuals where they accompany statements describing the hazards and advising how to avoid them This appendix presents the safety symbols and statements that apply to all of the products t...

Page 196: ...of an instrument or device When one of these symbols appear in a manual s narrative sections or procedures an accompanying statement identifies the applicable risk and explains how to avoid it Warning...

Page 197: ...xplosion Warning Risk of eye injury Warning To avoid injury from bursting nonmetallic tubing heed these precautions when working in the vicinity of such tubing when it is pressurized Wear eye protecti...

Page 198: ...otective panels The components they cover are not user serviceable Warning To avoid nonlethal electric shock ensure the mass spectrometer is in Standby mode before you touch any of its external surfac...

Page 199: ...titutes of Health NIH publication Biosafety in Microbiological and Biomedical Laboratories BMBL Observe Good Laboratory Practice GLP at all times particularly when working with hazardous materials and...

Page 200: ...appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample s integrity The exclamation point symbol and its associated statement alert you to such r...

Page 201: ...de la conformit la r glementation peut annuler le droit de l utilisateur exploiter l quipement Achtung Jedwede nderungen oder Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r di...

Page 202: ...me se trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique...

Page 203: ...il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de p...

Page 204: ...A Safety Advisories 204 February 11 2013 71500130002 Rev D Nonmetallic Tetrahydrofuran THF Methylene chloride Dimethyl sulfoxide THF...

Page 205: ...e par le mat riel risque d tre d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes die eingebauten Sicherheitseinrichtungen unter Umst nden...

Page 206: ...du type et de la puissance indiqu s sur le panneau proximit du couvercle de la boite fusible de l instrument Vorsicht Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen deren Typ und...

Page 207: ...ans la rubrique Remplacement des fusibles du chapitre traitant des proc dures de maintenance Vorsicht Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen deren Typ und Nennwert im Absc...

Page 208: ...cal symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument s front or rear panels Electrical power on Electrical power off Stand...

Page 209: ...1500130002 Rev D 209 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments devices and component parts are...

Page 210: ...A Safety Advisories 210 February 11 2013 71500130002 Rev D...

Page 211: ...pliant Solvents Warning To confirm the integrity of the source exhaust system you must address any safety issues raised by the contents of this Appendix Contents Topic Page Preventing contamination 21...

Page 212: ...out the most common ingredients used to prepare mobile phases Items exposed to solvent Item Material Autotune reservoirs High density polyethylene Corona discharge pin mounting contact PEEK Gas exhaus...

Page 213: ...oacetic acid 0 1 Ammonium acetate 10 mM Ammonium formate 10 mM These solvents are not expected to cause any problems with the materials identified in Items exposed to solvent on page 212 Trap bottle p...

Page 214: ...B Materials of Construction and Compliant Solvents 214 February 11 2013 71500130002 Rev D...

Page 215: ...trument or remove it from service contact Waters Technical Service for recommended cleaning flushing and packaging procedures See Troubleshooting with Connections INSIGHT on page 83 Contents Topic Pag...

Page 216: ...ons 1 10 1 LA N EPC Com Port Video Output Service Bus 1 2 3 4 5 6 7 8 9 10 Event In 1 ExternalConnections1 Not used CE Interlock OUT Event In 2 Not used IN IN Com N C N O Com N C N O 1 2 3 4 5 6 7 8 9...

Page 217: ...13 71500130002 Rev D 217 Connecting the Varian oil filled roughing pump Note To connect the alternative oil free roughing pump see Connecting the Edwards oil free roughing pump on page 223 TP03159 Inl...

Page 218: ...he Waters Rotary Pump Kit NW25 tee included in the installation kit NW25 center rings included in the installation kit NW25 clamps included in the installation kit 12 7 mm clear PVC exhaust tubing inc...

Page 219: ...access to the oil exhaust filter and oil level sight glass For proper ventilation the following minimum clearances must apply Warning To avoid personal contamination with biohazards or toxic material...

Page 220: ...ng an NW25 center ring and then secure the connection with an NW25 clamp using the 7 mm nut driver to install the clamp 5 Attach the flanged end of a length of 1 inch ID vacuum hose to the remaining o...

Page 221: ...aust vent 12 Inspect the oil level in the pump See Inspecting the Varian roughing pump oil level on page 101 and if needed Adding oil to the Varian roughing pump on page 102 13 Make the electrical con...

Page 222: ...the pump connector on the mass spectrometer s rear panel TP03159 TP03118 Auxiliary 10MB 100MB Activity External Connections 2 10 1 External Connections 1 10 1 LA N EPC Com Port Video Output Service B...

Page 223: ...ev D 223 Connecting the Edwards oil free roughing pump The oil free roughing pump is an alternative to the oil filled roughing pump To connect the oil filled roughing pump see Connecting the Varian oi...

Page 224: ...t NW25 center rings included in the installation kit NW25 clamps included in the installation kit NW40 center rings included in the installation kit NW40 clamps included in the installation kit NW25 N...

Page 225: ...orizontal The ambient temperature of area in which you place the pump must range between 12 and 40 C 54 to 104 F To ensure proper ventilation install the pump with the following minimum clearances TP0...

Page 226: ...ch the NW25 tee to the NW25 NW40 adaptor using an NW25 center ring and then secure the connection with an NW25 clamp using the 7 mm nut driver to install the clamp Warning To avoid personal contaminat...

Page 227: ...inch OD straight vacuum ports on the mass spectrometer s rear panel 8 Install 2 clamps on each hose end 9 Connect the 12 7 mm clear PVC exhaust tubing to the roughing pump exhaust port NW25 nozzle fit...

Page 228: ...the pump connector on the mass spectrometer s rear panel TP03118 Auxiliary 10MB 100MB Activity TP03165 External Connections 2 10 1 External Connections 1 10 1 LA N EPC Com Port Video Output Service Bu...

Page 229: ...lision cell gas inlet and vacuum connectors 2 Attach a nitrogen regulator not provided to the nitrogen supply TP03118 Auxiliary 10MB 100MB Activity External Connections 2 10 1 External Connections 1 1...

Page 230: ...and ferrule 1 8 inch stainless steel tube supplied with the mass spectrometer Argon regulator not supplied To connect the collision cell gas supply 1 Use the 1 8 inch Swagelok nut and ferrule to conne...

Page 231: ...bar 0 03 psi below atmospheric pressure negative pressure Warning To avoid personal contamination with biohazards or toxic compounds and to avoid spreading contamination to uncontaminated surfaces wea...

Page 232: ...to the nitrogen exhaust trap bottle 3 Connect one end of the tubing to the pilot valve port on the instrument s rear panel 4 Connect the free end of the tubing to the pilot valve port on the nitrogen...

Page 233: ...tubing into the outlet port on the nitrogen exhaust trap bottle 10 Route the free end of the tubing to the exhaust vent Requirement To confirm the integrity of the source exhaust system perform the f...

Page 234: ...r the barbed fitting of the drain located at the bottom left hand side of the mass spectrometer Warning To prevent personal contamination with biohazards or toxic materials always wear chemical resist...

Page 235: ...e so that its end is above the surface of the waste solvent Positioning of drain tube Warning To prevent leakage of biohazards or toxic materials ensure that the drain line does not crimp or bend impe...

Page 236: ...el of the mass spectrometer 3 Connect the other end of the shielded network cable to the port labeled instrument LAN on the workstation rear panel To connect the instrument to the power source Require...

Page 237: ...al Connections 2 are on the mass spectrometer s rear panel these connectors hold the screw terminals for input output signals The connectors are keyed so that they can receive a signal cable inserted...

Page 238: ...m Port Video Output System Activity Service Bus 1 2 3 4 5 6 7 8 9 10 Event In 1 ExternalConnections1 Not used CE Interlock OUT Event In 2 Not used IN IN Com N C N O Com N C N O 1 2 3 4 5 6 7 8 9 10 An...

Page 239: ...ommon 30 V d c 100 mA 9 CE Interlock Out normally closed N C 30 V d c 100 mA 10 CE Interlock Out normally open N O 30 V d c 100 mA External connections 2 Pin Function Rating 1 Analog Out instrument dr...

Page 240: ...rometer signal connections Signal connections Description Analog Out Used for analog chart output functionality The output voltage range is 0 to 2 V The resolution of the voltage output is 12 bits Gas...

Page 241: ...s of the signal cable to the connector 3 Slide the clamp with the bend facing down into the protective shield 4 Insert the clamp and shield with the bend facing down into the connection cover and loos...

Page 242: ...gnal cable into the connection cover and position the clamp over the cable leads and tighten the clamp into place with the second self tapping screw 6 Place the second connection cover over the first...

Page 243: ...long term input voltage stability To connect to the electricity source 1 Connect the female end of the power cord to the receptacle on the rear panel of the mass spectrometer 2 Connect the male end o...

Page 244: ...C External Connections 244 February 11 2013 71500130002 Rev D...

Page 245: ...r replacing the tubing in the IntelliStart Fluidics system Warning To avoid personal contamination with biohazards or toxic materials always wear chemical resistant powder free gloves while working on...

Page 246: ...ion on preventing contamination refer to Controlling Contamination in LC MS Systems part number 715001307 You can find this document on http www waters com click Services and Support Support Plumbing...

Page 247: ...external dimensions ED Color Length and Quantity for the IntelliStart Fluidics pipes Replacement pipe specifications Connection ID inches ED inches Color Length mm Quantity Select to divert 0 005 1 16...

Page 248: ...D Plumbing the IntelliStart Fluidics System 248 February 11 2013 71500130002 Rev D...

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