TRIZAIC UPLC
System Guide
Revision A
Copyright © Waters Corporation 2011
All rights reserved
Page 1: ...TRIZAIC UPLC System Guide Revision A Copyright Waters Corporation 2011 All rights reserved...
Page 2: ...ential damages in connection with or arising from its use Trademarks nanoACQUITY UPLC UPLC SYNAPT Xevo and Waters are registered trademarks of Waters Corporation and IntelliStart MassLynx NanoLockSpra...
Page 3: ...ganization s safety representative for guidance Considerations specific to the TRIZAIC UPLC System Consult the information on the documentation CD for additional safety considerations when using the s...
Page 4: ...e is intended for personnel who install operate or maintain the TRIZAIC UPLC System It provides an overview installation instructions and maintenance procedures Symbol Definition Manufacturer Authoriz...
Page 5: ...g mass spectrometers consult the calibration section of the operator s guide for the instrument you are calibrating In cases where an overview and maintenance guide not an operator s guide accompanies...
Page 6: ...vi EC authorized representative Waters Corporation Micromass UK Ltd Floats Road Wythenshawe Manchester M23 9LZ United Kingdom Telephone 44 161 946 2400 Fax 44 161 946 2480 Contact Quality manager...
Page 7: ...e and purpose iv Intended use of the TRIZAIC UPLC System v Calibrating the TRIZAIC UPLC System v ISM classification v ISM Classification ISM Group 1 Class B v EC authorized representative vi 1 TRIZAIC...
Page 8: ...software to control the system 2 11 Preparing the system 2 12 Loading samples in the sample manager 2 12 Setting temperatures 2 12 Calibrating the system 2 13 Installing a calibration nanoTile 2 13 Se...
Page 9: ...rnings that apply to all Waters instruments A 5 Electrical and handling symbols A 11 Electrical symbols A 11 Handling symbols A 12 B Materials of Construction and Compatible Solvents B 1 Preventing co...
Page 10: ...x Table of Contents...
Page 11: ...a TRIZAIC UPLC source The TRIZAIC UPLC source accepts a nanoTile device nanoTile which combines the functions of an analytical column trapping column and nanospray emitter This technology simplifies...
Page 12: ...ses and controls column temperature It also maintains status and usage of the nanoTile history number of injections maximum backpressure history and number of sample sets run System components A TRIZA...
Page 13: ...eter a flow splitter kit For a SYNAPT mass spectrometer source adaptor housing Installation DVD with nanoACQUITY UPLC March 2011 driver pack Documentation CD TRIZAIC UPLC System Guide TRIZAIC UPLC nan...
Page 14: ...e cross talk between the two sprays Data from the lock spray are used to calculate a correction factor for the mass scale calibration which is then applied to the sample data to provide exact mass inf...
Page 15: ...spheric pressure ionization API mass spectrometers See also SYNAPT series or Xevo series mass spectrometer documentation nanoACQUITY UPLC binary solvent manager A nanoACQUITY UPLC binary solvent manag...
Page 16: ...a waste line on the valve in the heating and trapping module Analysis When trapping is completed the gradient flows through the nanoTile s trap and analytical channels to elute and separate the analy...
Page 17: ...he TRIZAIC UPLC source You can install a TRIZAIC UPLC source on SYNAPT series or Xevo series mass spectrometers Preparing the instrument for work performed on its source Follow this procedure when a s...
Page 18: ...click Source Standby Exception For a Xevo TQ in the Tune window click API to stop the API gas flow See also The relevant instrument s operator s guide Removing the source enclosure from the mass spec...
Page 19: ...th biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shock prepare the instrument for work performed on it...
Page 20: ...TRIZAIC UPLC source on page 2 5 Installing adaptor housing on a SYNAPT source After removing the SYNAPT source enclosure install the SYNAPT source adaptor housing which provides supporting studs for t...
Page 21: ...porting studs on the source adaptor housing 2 Swing the source enclosure to the closed position using enough pressure to ensure it locks into place 3 Power off the sample manager and put the mass spec...
Page 22: ...e System TRIZAIC source connections Data power cable to QSPI connector on nanoACQUITY sample manager Fluid inlet line Fluid infusion line Fluid waste line High voltage cable Reference probe power cabl...
Page 23: ...ss spectrometer 5 Connect the high voltage cable white to the high voltage supply outlet on the mass spectrometer 6 Connect the reference probe power cable green to the reference probe power inlet on...
Page 24: ...the part numbers printed on their shrink wrap tubing Fluid line aperture TRIZAIC tubing assemblies Part Number Description 430002561 Infusion line Orange 430002351 Inlet line Orange 430002455 Waste l...
Page 25: ...s you must fit the supplied flow splitter kit to the fluidics valve before connecting the TRIZAIC fluid infusion line See also nanoACQUITY UPLC Operator s Guide and mass spectrometer documentation Sta...
Page 26: ...perate LED on mass spectrometers Status and Pressure LEDs on the TRIZAIC source LEDs on the TRIZAIC source The Stop Flow button on the TRIZAIC UPLC source stops fluid flow and depressurizes a nanoTile...
Page 27: ...ppear double click setup exe 4 Follow the instructions to install the TRIZAIC instrument driver and other instrument drivers for instruments in the system optional 5 Restart the sample manager See als...
Page 28: ...ding samples in the sample manager The sample manager accommodates sample trays and vial holders Carefully insert the vials into the wells of the sample tray and note their location for sample identif...
Page 29: ...s 2 Rotate the lever to engage the fluid and electronic connections Result The Status light blinks green when the nanoTile is correctly inserted into the TRIZAIC source s docking port The light shows...
Page 30: ...a 1 On the Tune window click Source TRIZAIC or Nanoflow 2 Click to open the Camera Control dialog box Camera Control view of sprayers and sample cone 3 Rotate the camera s focusing ring shown below to...
Page 31: ...ollows a Remove the spacer from the small space behind the Z control b Set the X Y and Z stages to the zero points on the micrometers Reason To ensure quality during shipping the source is shipped wit...
Page 32: ...djust for optimal signal 4 Mark the optimal position on the screen using the cross hairs on the viewing screen Tip The marked emitter position provides a guide when you insert the analytical nanoTile...
Page 33: ...ng calibration use the same flow rate as the intended flow rate If the gradient flow rate changes adjust the spray position and capillary voltage 5 Switch the baffle to the lock spray position to make...
Page 34: ...ments to the sprayer position can make large differences to the source sensitivity If spray instability occurs you can clean the emitter by washing with isopropanol and blowing it dry with air 6 Close...
Page 35: ...oTile To insert an analytical nanoTile 1 Insert a nanoTile that is suitable for your samples into the docking port on the TRIZAIC source 2 Rotate the lever counterclockwise 180 degrees to the vertical...
Page 36: ...enter the flow rate percentages of solvents A and B and sample loading time Limitation The high pressure limit is 34 500 kPa 345 bar 5000 psi 2 Create a sample manager instrument method Select Partia...
Page 37: ...ole select Sample Manager Maintain Remove nanoTile The pressure LED blinks green as the system is decompressing Requirement Do not proceed until the pressure LED is off 2 After the pressure LED goes d...
Page 38: ...2 22 Preparing the System...
Page 39: ...schedule and perform maintenance as required and described in this chapter See also TRIZAIC UPLC nanoTile Care and Use Guide nanoACQUITY UPLC System Operator s Guide Operator s overview and maintenan...
Page 40: ...g maintenance procedures Maintenance schedule Procedure Frequency For information Replace a fluid line As required or during periodic maintenance See page 3 4 Clean the instrument surface fluid connec...
Page 41: ...ce enclosure ensure that the following criteria are met Instrument is in Standby mode LC flow is diverted to waste or set to off Desolvation gas is turned off Caution To prevent damage to the nanoTile...
Page 42: ...sembly as a single unit No parts of the fluid line are reusable once detached Fluid line connections To identify the correct tubing assembly for each fluid line use the following table Tip You can use...
Page 43: ...TY UPLC instrument or from the grounded union connected to the reference valve on the mass spectrometer in accordance with the supplied documentation See also nanoACQUITY UPLC System Operator s Guide...
Page 44: ...rument for working on its source see page 2 1 2 Pull open the source enclosure door and then locate the fluid line connector plate 3 Identify the correct hole in the connector plate and thread the loo...
Page 45: ...LC System documentation See also nanoACQUITY UPLC System Operator s Guide 7 Attach the fluid line to the appropriate port on the nanoACQUITY UPLC instrument or to the onboard IntelliStart Fluidics on...
Page 46: ...n the source door 2 From the back gently direct a stream of air from a clean source across the electronic connectors Note To prevent contamining the inside of the source do not direct air into the sou...
Page 47: ...he nanoACQUITY UPLC sample manager 5 Disconnect the QSPI cable 6 Using the inch wrench loosen and disconnect the fluid waste line and fluid inlet lines from the sample manager see figure Plumbing conn...
Page 48: ...power cable green from the reference probe power inlet on the mass spectrometer 10 Disconnect the fluid infusion line from the onboard IntelliStart Fluidics on the mass spectrometer 11 Carefully lift...
Page 49: ...des also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and statements that apply t...
Page 50: ...electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying text identifies the specific risk and explains ho...
Page 51: ...tion Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran THF and nitric or sulfuric ac...
Page 52: ...al surfaces of the mass spectrometer when the instrument is in Operate mode To avoid nonlethal electric shock make sure the instrument is in Standby mode before touching areas marked with this high vo...
Page 53: ...When operating this device follow standard quality control procedures and the equipment guidelines in this section Warning Waters instruments can be used to analyze or process potentially hazardous s...
Page 54: ...er Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren Avv...
Page 55: ...e trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou n...
Page 56: ...iamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi n El usuari...
Page 57: ...Warnings that apply to all Waters instruments A 9 Nonmetallic Tetrahydrofuran THF Methylene chloride Dimethyl sulfoxide THF...
Page 58: ...tueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes die eingebauten Sicherheitseinrichtungen unter Umst nden nicht ordnungsgem funktionieren Atte...
Page 59: ...ls These can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor ter...
Page 60: ...visories Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright Keep dry Fragil...
Page 61: ...ww waters com and Controlling Contamination in LC MS Systems part number 715001307 Items exposed to solvent The items that appear in the following table can be exposed to solvent You must evaluate the...
Page 62: ...MS Water Methanol Acetonitrile Formic acid 0 1 Acetic acid 0 1 Trifluoroacetic acid 0 1 Ammonium acetate 10 mM Ammonium formate 10 mM These solvents are not expected to cause any problems with the mat...
Page 63: ...ry Contact Waters Technical Service for more information on specifications For nanoACQUITY UPLC System instruments see the TRIZAIC UPLC System documentation CD For a mass spectrometer see the document...
Page 64: ...ute Specification Protection classa a Protection Class I The insulating scheme used in the instrument to protect from electrical shock Class I identifies a single level of insulation between live part...
Page 65: ...by condensation d Moisture Protection Normal IPXO IPXO means that no Ingress Protection against any type of dripping or sprayed water exists The X is a placeholder that identifies protection against...
Page 66: ...C 4 Specifications...
Page 67: ...B 1 construction materials B 1 contamination preventing B 1 corona pin tip 2 3 cross talk 1 4 cross hairs 2 16 D data power cable 2 6 2 7 degassing solvents 2 12 depressurizing a nanoTile device 2 10...
Page 68: ...rked emitter position 2 16 2 20 mass spectrometer specifications C 1 mass spectrometer shock hazard A 4 MassLynx inlet method 2 12 operating software 2 11 sample list 2 20 shutdown method 2 18 version...
Page 69: ...2 18 setting up camera 2 14 sheath nanoTile device 2 19 2 21 shutdown method MassLynx 2 18 single pump trapping 1 6 solvents compatible B 1 degassing 2 12 exposure of components to B 1 use in mobile...
Page 70: ...on 2 9 W warning symbols A 2 A 5 waste line fluid 2 6 replacing 3 4 waste line fluid 2 9 working on source 2 1 X X axis range 2 16 Xevo TQ mass spectrometer 2 2 2 9 Xevo TQ MS flow splitter kit 1 3 Y...
