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Electrical and handling symbols

A-13

Electrical and handling symbols

Electrical symbols

These can appear in instrument user manuals and on the instrument’s front
or rear panels.

Electrical power on

Electrical power off

Standby

Direct current

Alternating current

Protective conductor terminal

Frame, or chassis, terminal

Fuse

Recycle symbol: Do not dispose in municipal waste.

Summary of Contents for TQ Detector

Page 2: ...for any errors that may appear in this document This document is believed to be complete and accurate at the time of publication In no event shall Waters Corporation be liable for incidental or conse...

Page 3: ...r to suggest ideas for otherwise improving it Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability We seriously consid...

Page 4: ...This protocol addresses good operating procedures and the techniques necessary to validate system and method performance Instrument specific safety considerations Solvent leakage hazard The source exh...

Page 5: ...en supply fails High temperature hazard Waters TQ Detector high temperature hazard Warning Where significant quantities of flammable solvents are involved the source must receive a continuous flow of...

Page 6: ...The components they cover are not user serviceable To avoid non lethal electric shock any equipment connected to the ESI and IonSABRE APCI probes must be grounded When the TQ Detector is in Operate mo...

Page 7: ...modes The Waters TQ Detector can be used for general In Vitro Diagnostic applications only by professionally trained and qualified laboratory personnel Symbol Definition Authorized Representative of t...

Page 8: ...nd run to run Data collected when quality control samples are out of range might not be valid Do not report these data until you are certain that the instrument performs satisfactorily When analyzing...

Page 9: ...the United Kingdom with the Medicines and Healthcare Products Regulatory Agency MHRA at market Towers 1 Nine Elms Lane London SW8 5NQ The reference number is IVD000167 Waters Corporation Micromass UK...

Page 10: ...x...

Page 11: ...ntative information ix IVD authorized representative ix 1 Waters TQ Detector Overview 1 1 Overview 1 2 Waters TQ Detector 1 2 ACQUITY TQD UPLC MS system 1 4 MassLynx mass spectrometry software and dat...

Page 12: ...ystem from the Tune window 1 18 Programming the MS method to operate the IntelliStart fluidics system 1 18 2 Preparing the Waters TQ Detector for Operation 2 1 Starting the TQ Detector 2 2 Configuring...

Page 13: ...e ESI probe for ESCi operation 3 7 Required materials 3 7 Removing the corona pin 3 9 Required materials 3 9 Removing the ESI probe 3 11 Required materials 3 11 4 Operating the Waters TQ Detector 4 1...

Page 14: ...xiv Table of Contents Caution symbol A 5 Warnings that apply to all Waters instruments A 5 Electrical and handling symbols A 13 Electrical symbols A 13 Handling symbols A 14...

Page 15: ...d plumbing connections Contents Topic Page Overview 1 2 Ionization techniques and source probes 1 7 Ion optics 1 9 MS operating modes 1 10 MS MS operating modes 1 11 Sample inlet 1 14 Vacuum system 1...

Page 16: ...speeds compatible with ultra performance LC Waters provides these ion sources with the instrument as standard equipment ZSpray dual orthogonal sampling interface Multi mode ESCi ionization switching...

Page 17: ...software IntelliStart enables simplified set up of the system for use in routine analytical and open access applications The IntelliStart fluidics system is built into the TQ Detector It delivers samp...

Page 18: ...UPLC system and the Waters TQ Detector ACQUITY UPLC system The ACQUITY UPLC system includes a binary solvent manager sample manager column heater optional sample organizer optional detectors and a sp...

Page 19: ...analog and mass spectrometry data MassLynx software permits these major operations Configuring the instrument Creating LC inlet and MS MS methods that define operating parameters for a run Using Intel...

Page 20: ...ole The ACQUITY UPLC Console is a software application with which you configure settings monitor performance run diagnostic tests and maintain the system and its modules The ACQUITY UPLC Console funct...

Page 21: ...The multiply charged ions are of particular interest because the TQ Detector separates them according to their mass to charge ratios m z permitting the detection of high molecular weight compounds The...

Page 22: ...hemical ionization APCI probe is offered as an option See also The Waters TQ Detector Operator s Guide for full details Atmospheric pressure photoionization Atmospheric pressure photoionization APPI i...

Page 23: ...rge ratio m z The mass separated ions pass into the T Wave collision cell where they either undergo collision induced dissociation CID or pass to the second quadrupole Any fragment ions are then mass...

Page 24: ...n switching rapidly between MS and MS MS operation for example in survey scan mode It also provides a useful tool for instrument tuning and calibration before MS MS analysis and for fault diagnosis Th...

Page 25: ...t ion mode Typical applications Structural elucidation for example peptide sequencing MS MS operating modes Operating mode MS1 Collision cell MS2 Product daughter ion spectrum Static at precursor mass...

Page 26: ...hat is to complement or confirm product scan data by scanning for all the precursors of a common product ion Multiple reaction monitoring mode MRM mode is a highly selective MS MS equivalent of SIR As...

Page 27: ...R a chromatogram is produced Constant neutral loss mode Constant neutral loss mode detects the loss of a specific neutral fragment or functional group from an unspecified precursor or precursors The s...

Page 28: ...by direct or combined infusion Vacuum system An external roughing rotary vane pump and an internal split flow turbomolecular pump combine to create the source vacuum The turbomolecular pump evacuates...

Page 29: ...65444751 VACUUM SOURCE VENT API GAS 6 9 BAR MAXIMUM V 200 240 Hz 50 60 VA 1200 RS 232 ETHERNET PUMP COLLISION GAS 1 0 BAR MAXIMUM IVD Not Used Switch 4 Ground Ground Stop Flow Switch 4 Ground Switch 3...

Page 30: ...to enable optimization at analytical flow rates The IntelliStart system incorporates a multi position valve with these attributes An input connection from an external LC column An input connection fr...

Page 31: ...and extent of the automation you want IntelliStart to perform See also The TQ Detector online Help for further details of IntelliStart Operating the IntelliStart fluidics system from the ACQUITY UPLC...

Page 32: ...pties When the syringe is empty the system becomes idle 14 Click to refill the syringe or to purge the system 15 Click to stop the current action Operating the IntelliStart fluidics system from the Tu...

Page 33: ...ts 3 In the Method events dialog box select the appropriate flow state as shown in the following table Tip At instrument power up the LC state is waste See also MassLynx online Help topic Advanced Met...

Page 34: ...1 20 Waters TQ Detector Overview...

Page 35: ...or Operation This chapter describes how to start and shut down the TQ Detector Contents Topic Page Starting the TQ Detector 2 2 Preparing the IntelliStart fluidics system 2 9 Rebooting the TQ Detector...

Page 36: ...must be dry and oil free with a purity of at least 95 Regulate the supply at 600 to 690 kPa 6 0 to 6 9 bar 90 to 100 psi See also Figure titled TQ Detector rear panel on page 1 15 2 Ensure that the c...

Page 37: ...power LED shows green During initialization the binary solvent manager s and sample manager s status LED flashes green After the instruments are successfully powered on all power LEDs show steady gre...

Page 38: ...for Operation 8 Click Control Pump to start the roughing pump The operate LED remains off Tip There is a 20 second delay during which the turbopump is starting before the roughing pump starts Intelli...

Page 39: ...ode When the TQ Detector is in good operating condition IntelliStart displays Ready Tip Clicking Resolve should prepare the system for operation putting the TQ Detector into Operate mode If clicking R...

Page 40: ...or an item highlight it and click Properties 4 Click Apply OK Verifying the instrument s state of readiness When the TQ Detector is in good operating condition the power LED shows constant green and t...

Page 41: ...s IntelliStart automatically sets these when you enter a flow rate according to the following table Note Under low ambient temperature high moisture and high flow rate conditions condensation can occu...

Page 42: ...he system is waiting for an instrument component to reach operational conditions The LED could for example flash while the system reaches a programmed temperature set point or vacuum level The instrum...

Page 43: ...olvent manifold drip tray Required materials Chemical resistant powder free gloves To install the solvent manifold drip tray Install the solvent manifold drip tray as shown below Warning The solvent m...

Page 44: ...r free gloves To install the reservoir bottles 1 Remove the reservoir bottle caps 2 Screw the reservoir bottles onto the TQ Detector as shown below Warning The reservoir bottles can be contaminated wi...

Page 45: ...d waste is open The syringe is empty and the flow path between it and waste is open LC position In the LC position the flow path between the LC and probe is open and the flow path between the syringe...

Page 46: ...nfusion position in infusion mode Combined position with LC flow and syringe in idle mode Column Infusion LC Waste Probe Syringe Reservoir A Reservoir B Off On B A Infusion Column Combined LC Waste Pr...

Page 47: ...the infusion syringe with the solution that you are going to use next Tip Depending on the solutions used the IntelliStart fluidics system can require more then one purge cycle to minimize carryover T...

Page 48: ...nitialize Immediately following software download Rebooting the TQ Detector by pressing the reset button Tip The reset button shuts down the electronics momentarily and causes the TQ Detector to reboo...

Page 49: ...t shutdown You might want to shut down the instrument for a relatively brief period like overnight while maintaining the LC flow To put the TQ Detector in Standby mode 1 In the ACQUITY UPLC Console cl...

Page 50: ...valve opens and the instrument is automatically vented The operate LED changes from green to red and then turns off 5 Exit MassLynx 6 After the roughing pump shuts off operate the power button on the...

Page 51: ...n the front of the TQ Detector 2 Disconnect the power cable from the back of the TQ Detector Warning The TQ Detector s power switch does not isolate the instrument from the main power supply To isolat...

Page 52: ...2 18 Preparing the Waters TQ Detector for Operation...

Page 53: ...i combined electrospay and atmospheric pressure chemical ionization If your system uses APCI mode refer to Chapter 6 of the Waters TQ Detector Operator s Guide Contents Topic Page Introduction 3 2 Ins...

Page 54: ...7 Installing the ESI probe Required materials Chemical resistant powder free gloves To install the ESI probe 1 Prepare the instrument for working on the source See also Chapter 5 of the Waters TQ Det...

Page 55: ...e the ESI probe into the hole in the probe adjuster assembly 5 Secure the ESI probe by tightening the two thumbscrews See also Figure titled ESI probe mounted on the source enclosure showing the conne...

Page 56: ...r ID Tip Two tubes of different ID are supplied with the instrument Requirement If you are replacing the tubing supplied with the instrument you must minimize the length of the tube connecting the div...

Page 57: ...probe and source can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shock ensure...

Page 58: ...pin to the mounting contact Requirement Ensure that the corona pin is securely mounted and that its tip aligns with the sample cone orifice Warning The corona pin tip is sharp To avoid puncture wound...

Page 59: ...e showing the connections to the front panel on page 3 4 7 Close the source enclosure door and fasten the handle 8 Close the instrument s access door Optimizing the ESI probe for ESCi operation Requir...

Page 60: ...nd Corona A in the current mode or kV in the voltage mode to produce the most intense ESI and APcI signal 13 Use the vernier probe adjuster to gradually move the probe bi directionally to determine th...

Page 61: ...source can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shock ensure that the i...

Page 62: ...plug to the corona pin mounting contact See also Figure titled Corona pin mounting contact on page 3 6 6 Close the source enclosure door and fasten the handle 7 Close the instrument s access door Warn...

Page 63: ...6 Undo the two thumbscrews securing the ESI probe to the probe adjuster assembly 7 Carefully remove the ESI probe from the probe adjuster assembly Warning The ACQUITY UPLC system connections ESI probe...

Page 64: ...3 12 ESI and ESCi Modes of Operation 8 If available fit the protective sleeve to the ESI probe tip 9 Close the instrument s access door...

Page 65: ...Detector it explains these tasks Setting up your TQ Detector Performing a sample tune Developing instrument methods Verifying the system Contents Topic Page Setting up the instrument 4 2 Performing a...

Page 66: ...aterials Sodium cesium iodide solution 2 ng L Sulfadimethoxine solution 100 pg L To prepare the IntelliStart fluidics system See also IntelliStart fluidics system operation on page 1 17 1 Ensure that...

Page 67: ...1 In the ACQUITY UPLC Console system tree click TQ Detector 2 Ensure that Ion Mode is ES 3 In the ACQUITY UPLC Console system tree click IntelliStart 4 Ensure that Instrument Setup is selected 5 If yo...

Page 68: ...every time you select a new calibration reference file Instrument Setup Parameters dialog box Instrument Setup tab with default parameters Tip You can use alternative reference solutions to calibrate...

Page 69: ...the Sample Tune tab 2 Click Copy Instrument Setup masses Rationale The Tune Masses from the Instrument Setup tab are copied into the Sample Tune Masses boxes Instrument Setup Parameters dialog box Sa...

Page 70: ...rt instrument set up 1 Click Start Result A message appears reminding you to ensure that the calibrant solutions calibration parameters and LC flow are set correctly 2 Click OK Result Instrument set u...

Page 71: ...e four masses defined in the IntelliStart Setup Parameters dialog box When the sample tune is complete the results are written to the Sulfadimethoxine ipr file Performing a sample tune In the followin...

Page 72: ...valid If so no sample tune is performed See also TQ Detector online Help topic IntelliStart flow diagram 7 Click Start 8 In the IntelliStart Setup Parameters dialog box s Sample Tune tab specify the...

Page 73: ...solution in reservoir B See also To prepare the IntelliStart fluidics system on page 4 2 2 In the ACQUITY UPLC Console system tree click TQ Detector 3 Ensure that the Ion Mode is ES 4 In the ACQUITY U...

Page 74: ...rs reminding you to ensure that the sample solutions sample tune parameters and LC flow are set correctly 12 Click OK Result Method development starts The ACQUITY UPLC Console displays the progress of...

Page 75: ...e sequence or automatically based on a scheduled date and time Multiple injections are processed using the OpenLynx Application Manager Measurements are reported for each chosen parameter across the r...

Page 76: ...method files from the System QC project SystemQC pro You must save the tune inlet and method files to the Aqudb folder of the System QC project before performing the verification To perform a manual...

Page 77: ...ning method enter a Mass on which to perform peak detection 6 Select Print Report to print a report of the results 7 Select the Acceptance parameters Instrument Setup Parameters dialog box System QC t...

Page 78: ...ears reminding you to ensure that the sample vial QC parameters and LC flow are set correctly 9 Click OK Result System QC starts The ACQUITY UPLC Console displays its progress Signal Noise In Signal N...

Page 79: ...des also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and statements that apply t...

Page 80: ...ks include burn injuries electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying text identifies the spec...

Page 81: ...around such tubing Wear eye protection Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydro...

Page 82: ...l surfaces of the mass spectrometer when the instrument is in Operate mode To avoid non lethal electric shock make sure the instrument is in Standby mode before touching areas marked with this high vo...

Page 83: ...hen operating this device follow standard quality control procedures and the the equipment guidelines in this section Warning Waters instruments can be used to analyze or process potentially hazardous...

Page 84: ...n oder Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren...

Page 85: ...e trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou n...

Page 86: ...no rigonfiamento nei tubi non metallici riducendo notevolmente la resistenza alla rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi...

Page 87: ...Warnings that apply to all Waters instruments A 9...

Page 88: ...isque d tre d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes unter Umst nden nicht ordnungsgem funktionieren Attenzione l utente deve ess...

Page 89: ...de la m me puissance afin d viter tout risque d incendie Vorsicht Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen Attenzione per una buona p...

Page 90: ...prise avant d effectuer la maintenance de l instrument Vorsicht Zur Vermeidung von Stromschl gen sollte das Ger t vor der Wartung vom Netz getrennt werden Attenzione per evitare il rischio di scossa e...

Page 91: ...ls These can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor ter...

Page 92: ...visories Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright Keep dry Fragil...

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