Verifying the system using System QC
4-13
3.
In the IntelliStart Setup Parameters dialog box’s System QC tab, enter
the number of pre-run and run injections, the vial (that is, the position of
your sample in the Autosampler) and the injection volume.
Tip:
At least two injections are required for a run, because some of the
validation parameters use standard deviation. A pre-run sample is used
to clean and stabilize the system. You need not perform a pre-run prior
to a run.
Recommendation:
Perform at least three injections.
4.
Enter a tune, LC and inlet file, or use the browse button to select a file.
5.
Select whether you are using a Scanning or SIR/MRM method. If using a
scanning method, enter a Mass on which to perform peak detection.
6.
Select Print Report to print a report of the results.
7.
Select the Acceptance parameters.
Instrument Setup Parameters dialog box System QC tab Acceptance
parameters :
Parameter
Description
Retention Time
Enter the expected retention time in the Set
Point box and enter the tolerance. If, for any
sample, the peak top is outside the range, the
test reflects a failure.
Peak Area
The variation in peak area is calculated by
percentage Relative Standard Deviation
(%RSD).
%RSD = (Standard Deviation/Average Area) ×
100%
Enter a tolerance in %RSD, above which the
test reflects a failure.
Peak Height
This is similar to Peak Area but using Peak
Height instead.
Peak Width
Enter the expected peak width in the Set Point
box and enter the tolerance. If, for any sample,
the peak width falls outside the range, the test
reflects a failure.