Developing experiment methods
4-9
Developing experiment methods
IntelliStart allows you to automatically develop quantitative MRM or SIR
methods for compounds of interest. Up to four compounds can be handled in a
single process. Up to three transitions can be selected for each compound.In
this example, methods for sulfadimethoxine are created.
Required materials
Sulfadimethoxine solution (100 pg/µL)
To create a method:
1.
Prepare the IntelliStart fluidics system with sulfadimethoxine solution
in reservoir B.
See also:
“To prepare the IntelliStart fluidics system:” on page 4-2
2.
In the ACQUITY UPLC Console system tree, click TQ Detector.
3.
Ensure that the Ion Mode is ES+.
4.
In the ACQUITY UPLC Console system tree, click IntelliStart.
5.
Clear the Instrument Setup check box.
6.
Click Develop Method.
7.
If you require system pre-checking, click Pre-checks.
See also:
TQ Detector online Help topic “IntelliStart flow diagram”.
8.
Click Start
.
9.
In the IntelliStart Setup Parameters dialog box, click the Method
Developer tab.
Note:
The masses, tune file and IntelliStart fluidics system parameters
already set in IntelliStart are used.
10. Select the parameters shown in the following figure.