Setting-up the instrument
4-3
8.
Click OK.
9.
Click
to purge the system.
Tip:
System purge takes approximately 2 minutes.
10. Repeat
Rationale:
Purging the IntelliStart fluidics system twice ensures that
any traces of previously used solutions are removed from the system.
11. Click on the currently-selected reservoir display.
12. In the Select Reservoir dialog box, select the reservoir not currently
selected.
13. Click OK.
Result:
The other reservoir is selected, and the system is purged.
14. When the purge finishes, click
to purge the system again.
To specify the instrument set-up parameters:
1.
In the ACQUITY UPLC Console system tree, click TQ Detector.
2.
Ensure that Ion Mode is ES+.
3.
In the ACQUITY UPLC Console system tree, click IntelliStart.
4.
Ensure that Instrument Setup is selected.
5.
If you require system pre-checking, click Pre-checks.
Rationale:
If Pre-checks is selected, when IntelliStart starts the
instrument set-up, it determines whether the existing calibration is still
valid. If so, it does not perform a full calibration but proceeds to sample
tuning the instrument.
See also:
TQ Detector online Help topic “IntelliStart flow diagram”.
6.
Click Start
to open the IntelliStart Setup Parameters dialog box.
7.
In the Instrument Setup tab’s Reference drop-down list, click Naics.
Rationale:
Naics is the calibration reference file for sodium cesium
iodide when working in ES+ ion mode.