1-12
Waters TQ Detector Overview
•
Method development for MRM screening studies:
–
Identifying product ions for use in MRM transitions.
–
Optimizing CID tuning conditions to maximize the yield of a specific
product ion to be used in MRM analysis.
Precursor (parent) ion mode
Precursor ion mode:
Typical application
You typically use the precursor ion mode for structural elucidation–that is, to
complement or confirm product scan data–by scanning for all the precursors of
a common product ion.
Multiple reaction monitoring mode
MRM mode is a highly selective MS/MS equivalent of SIR. As both MS1 and
MS2 are static, greater dwell time on the ions of interest is allowed, and
therefore better sensitivity compared to scanning MS/MS. This is the most
commonly used acquisition mode for quantitative analysis, allowing the
compound of interest to be isolated from the chemical background noise.
MS1
Scanning
MS2
Static (at product mass)
Collision cell
Pass all masses