Waters Micromass ZQ 2000, Operator'S Manual, Page: 46 / 133

Tuning 46
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6. Ensure the drop-down list displays Hamilton 250uL, then click OK .
7. Set the syringe pump to deliver 10 µL/min by entering that rate in the Pump Flow
field of the Analyser page (
Figure 3-5 ).
8. Click (Syringe pump) to infuse the startup solution into the source.
9. Click the Analyser tab, and ensure the syringe pump flow rate is 10 µL/min.
10. Turn the probe adjustor (see Figure 2-8 ) in one direction or the other to optimize
peak intensities.
11. Adjust the capillary, cone, extractor, and RF lens voltages to optimize peak
intensities.
3.2.2 Specifying Parameter Settings on the Analyser Page
Analyser settings optimize mass peak resolution. With span settings at 5, the bases of
mass peaks as they appear on the Tune window Analyser page should measure 1 da.
Note: The parameters LM Resolution, HM Resolution, and Ion Energy optimize resolution.
Table 3-3 describes the Tune window’s Analyser page parameters.
1. Click the Analyser tab. The Tune window Analyser page appears ( Figure 3-5 ).
Table 3-3 Analyser Page Parameters
Parameter Description
LM Resolution
HM Resolution
Affect mass peak concentration. Usually, 15, an arbitrary
unit, yields adequate mass resolution. Increasing the value
lowers sensitivity; decreasing it enhances sensitivity.
Ion Energy (V) Decreases resolution. Set between –1 and 3 V, as low as
possible without reducing peak intensity.
Multiplier (V) Modifies gain (attenuation). Settings between 400 and 450
yield a suitable signal-to-noise balance.