Inspecting the APCI Probe
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6.3 Inspecting the APCI Probe
1.
Switch the instrument to
Standby
from the Tune window.
2.
Make sure the probe heater is off.
3.
Unplug the probe from the instrument front panel, and remove it from the source.
4.
Connect the PTFE tube to the nebulizer outlet on the front panel.
5.
Remove the probe heater assembly, carefully loosening the two set screws.
6.
Disconnect the heater from the probe body by pulling it parallel to the axis of the
probe.
7.
Ensure that 0.5 to 1 mm of fused silica protrudes from the stainless steel nebulizer
tube.
8.
Connect the LC pump to the probe with a flow of acetonitrile : water, 1:1, at
1 mL/min.
9.
Make sure the liquid jet flows freely from the end of the capillary and that the LC
pump pressure reads 250 to 400 psi.
10. Check that the nitrogen supply pressure is 90 to 100 psi (6 to 7 bar).
11. Click
(API Gas) to start the nitrogen flow.
12. Make sure the liquid jet converts to a fine, uniform aerosol.
13. Stop the liquid flow.
14. Click
(API Gas) to stop the nitrogen flow.
15. Reconnect the probe heater assembly.
16. Insert the APCI probe into the source, securing it by tightening the two
thumbscrews.
17. Connect the probe cable to APCI/ESI on the instrument’s front panel. (Note that you
must first remove the plug labeled ESI.)
Summary of Contents for Micromass ZQ 2000
Page 6: ......
