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sample solutions are essential for UPLC analysis. See
for recommendations
on choosing and handling solvents.
2.7.2 Matching sample diluents
When you use the sample manager's auto-dilution option, the purge solvent serves as the sample
diluent. Ensure that your sample solution is soluble and miscible in your chosen purge solvent.
2.8 Transferring methods
Transferring an LC method from one system to another can sometimes be necessary. For such
transfers, the object is to maintain a separation’s performance or enhance it by reducing runtime
or improving selectivity and/or resolution. The ACQUITY UPLC H-Class system, which Waters
designed specifically to facilitate method transfer, are the ideal tools to achieve that goal with
quaternary solvent capability, low pressure mixing, and flow through needle design. The optional
column manager facilitates switching between target columns while exhibiting low bandspread
and maintaining the same temperature profile as the standard column heater.
The following example of a method transfer shows the related substances test for galantamine,
an alkaloid used to treat Alzheimer’s disease. The USP method (monograph: USP32-NF27
supplement: no. 2, page 4245) is demonstrated first using an HPLC system. The method is then
transferred to the system fitted with an HPLC column, whereby selectivity and resolution are
maintained. Using the ACQUITY UPLC columns calculator, it is then scaled to UPLC and
optimized for the shortest analysis time at equal peak capacity. The run time decreased by 46
minutes.
August 8, 2016, 715005049 Rev. C
Page 42