cycle time for small volume injections. Gradients pass through the needle during injection,
ensuring complete sample recovery.
1.7.3 Sample manager-FL (SM-FL)
The sample manager - fixed loop (SM-FL) uses a loop-based mechanism to inject samples. After
a puncture needle pierces the well or vial covering, a sample needle emerges from within the
puncture needle to aspirate the sample. In the partial loop needle overfill injection mode (default),
the sample needle is then removed from the vial and the sample syringe continues to pull the
sample aliquot through the sample needle and through the injection valve until any pre-sample
and the sample injection volume pass through the injection valve. The valve actuates, switching
the sample loop to the load position. The sample is pushed back toward the needle and the
sample volume is then pushed into the sample loop. The sample loop is moved to the injection
position and flow from the pump pushes the sample onto the column.
See also:
ACQUITY UPLC Sample Manager - Fixed Loop PLUS Overview and Maintenance
Guide
on your documentation media, or at
, for more information on other injection
modes.
1.7.4 Column heater (CH-A)
Column temperature variations can shift peak retention times and alter peak shapes, increasing
the difficulty of achieving precise results. The ACQUITY UPLC I-Class Series system’s column
heater (CH-A) helps to ensure precise, reproducible separations by controlling the column
temperature. The CH-A heats the column compartment to any temperature from 20 ºC to 90 ºC,
that is at least 5 °C above the ambient temperature. A low dispersion, active preheating device
heats the incoming solvent before it enters the column. The column compartment can
accommodate columns of up to 4.6-mm I.D. and up to 150-mm length.
1.7.5 30-cm Column heater (optional)
The optional 30-cm column heater (CH-30A) heats the column compartment to any temperature,
from 20 ºC to 90 ºC, that is at least 5 ºC above the ambient temperature. The CH-30A mounts on
the side of the system stack.
The CH-30A can accommodate UPLC columns of 4.6 mm, or less, ID and 150 mm, or less,
length and HPLC columns of 4.6 mm, or less, ID and 300 mm, or less, length. For columns that
exceed 4.6 mm ID, use the ACQUITY UPLC 30-cm column heater/cooler (30cm CHC) with the
appropriate compatibility kit.
Note:
The ACQUITY UPLC 30cm CHC is compatible with ACQUITY UPLC I-Class Series
systems. See
ACQUITY UPLC Column Compartments Operator's Overview and Maintenance
Information
on your documentation media, or at
, for details.
April 4, 2018, 715005704 Rev. A
Page 19
