Figure 1–2: Comparison of chromatographic separations using 5.0-µm and 1.7-µm
particles
The figure above compares two separations, one using HPLC with a column packed with 5 µm
particles, and the other using UPLC with a column packed with 1.7 µm particles. The
improvements in both resolution and the speed of analysis are apparent in the UPLC
chromatogram. Each separation was performed on a 2.1 × 50 mm column. Chromatographic
conditions for the separations were identical, except for the flow rate, which was scaled based on
particle size.
1.2 Features of the ACQUITY UPLC I-Class Series system
The ACQUITY UPLC I-Class Series system significantly improves the results of traditionally
challenging separations.
When compared to systems typically used for difficult assays, the I-Class Series system offers
these advantages:
• Significantly decreased system dispersion
• Rapid injection cycles and sample throughput
• Greatly reduced carryover
These advantages provide the following benefits over traditional systems:
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