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ACQUITY UPLC I-Class Series
System

Waters designed the ACQUITY UPLC I-Class Series system for optimum performance when
running difficult assays. Configure it with a mass spectrometer to take full advantage of the
system’s design enhancements.

1.1 UPLC technology

In 2004, Waters made significant advances in instrumentation and column design to introduce
UPLC technology to the field of separation science. By employing this technology, Waters’
ACQUITY UPLC systems achieve a marked increase in resolution, speed and sensitivity in liquid
chromatography when compared to conventional systems.

UPLC technology is based on columns packed with 1.7 µm-diameter, spherical particles coupled
with low dispersion systems, allowing you to realize the full separation potential of these highly
efficient columns.

April 4, 2018, 715005704 Rev. A

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Summary of Contents for ACQUITY UPLC I-Class Series

Page 1: ...ACQUITY UPLC I Class Series System Guide 715005704 Revision A Copyright Waters Corporation 2018 All rights reserved...

Page 2: ...Waters website waters com Trademarks ACQUITY is a registered trademark of Waters Corporation ACQUITY UPLC is a registered trademark of Waters Corporation Analyst is a registered trademark of Applied B...

Page 3: ...ters Corporation XBridge is a registered trademark of Waters Corporation Waters is a registered trademark of Waters Corporation All other trademarks are property of their respective owners Customer co...

Page 4: ...tentially hazardous effects of all substances you work with Always follow Good Laboratory Practice GLP and consult your organization s standard operating procedures as well as your local requirements...

Page 5: ...void injury from electrical shock or fire and damage to the equipment follow these guidelines Do not expose the workstation or ancillary equipment to dripping or splashing liquids Do not place objects...

Page 6: ...quality control QC procedures and the guidelines presented in this section Applicable symbols The following symbols can be present on the device system or packaging Symbol Definition Manufacturer Date...

Page 7: ...he system is for research use only and is not intended for use in diagnostic applications Calibrating To calibrate LC systems adopt acceptable calibration methods using at least five standards to gene...

Page 8: ...irements Group 1 products apply to intentionally generated and or used conductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class B products are...

Page 9: ...afety notice v Equipment misuse notice vi Safety advisories vi Operating the system vi Applicable symbols vi Audience and purpose vii Intended use of the ACQUITY UPLC I Class Series system vii Calibra...

Page 10: ...lexCart optional 21 1 7 11 Column technology 21 1 7 12 Detection 22 1 8 For additional information 22 2 Performance optimization 24 2 1 General guidelines 24 2 2 Dispersion 25 2 3 Carryover 26 2 4 Con...

Page 11: ...2 Sample manager control panel 50 3 5 3 TUV control panel 51 3 5 4 Column manager control panel 52 A External connections 54 A 1 Ethernet connections 54 A 2 Column heater connection 54 A 3 External c...

Page 12: ...ents and devices 71 B 4 Electrical and handling symbols 76 B 4 1 Electrical symbols 76 B 4 2 Handling symbols 76 B 5 Stacking system modules with interlocking features 77 B 6 Stacking system modules w...

Page 13: ...ntation and column design to introduce UPLC technology to the field of separation science By employing this technology Waters ACQUITY UPLC systems achieve a marked increase in resolution speed and sen...

Page 14: ...ure above that using 1 7 m particles achieves higher efficiency that persists as flow rate increases lower HETP indicates higher efficiency When operating in this area of the plot the peak capacity an...

Page 15: ...conditions for the separations were identical except for the flow rate which was scaled based on particle size 1 2 Features of the ACQUITY UPLC I Class Series system The ACQUITY UPLC I Class Series s...

Page 16: ...ds to other systems UPLC applications can benefit from pre column mobile phase heating to improve chromatographic separations The ACQUITY UPLC I Class Series column compartments use an active preheate...

Page 17: ...ation You also benefit from improvements in sensitivity and flexibility See also Waters com for more information on compatible Waters mass spectrometers 1 7 System components The following illustratio...

Page 18: ...6 kPa 1241 bar 18 000 psi which falls within the range of optimal linear velocity for sub 2 m particle columns of ID 1 0 mm to 3 0 mm See also ACQUITY UPLC Binary Solvent Manager PLUS Overview and Mai...

Page 19: ...ature variations can shift peak retention times and alter peak shapes increasing the difficulty of achieving precise results The ACQUITY UPLC I Class Series system s column heater CH A helps to ensure...

Page 20: ...to 65 0 C The 30 cm CHC module shares many of the same components with the 30 cm CH module except for a thermoelectric engine for heating and cooling and an additional fan for exhausting engine heat I...

Page 21: ...ides electrical outlets for system instruments and integrated waste management Used with a mass spectrometer the cart s adjustable height lets you position the column outlet close to the inlet probe m...

Page 22: ...ificate of analysis QC test data When you attach the column s eCord fob to the receptacle on the column compartment the chip automatically records and stores system information You need take no furthe...

Page 23: ...find more information and to join the ACQUITY UPLC online community where you can Share information and ask questions of ACQUITY UPLC experts and scientists Access ACQUITY UPLC publications and user...

Page 24: ...nalyses a peak of interest can be less than 0 5 seconds Waters recommends a sampling rate of 25 to 50 points across the peak which provides good quantitation and peak representation Sampling rates fas...

Page 25: ...on UPLC systems exhibit low dispersion a fixed instrument characteristic measured by the extent of peak broadening which occurs because of the system volume Small particle chromatography uses small hi...

Page 26: ...ad Ahead and Loop Offline options Initiating these options before the highly organic part of the gradient reaches the needle can leave sample residue in the system The time savings you gain from these...

Page 27: ...specifically that different techniques apply to retightening fittings versus installing them for the first time See also The user documentation for the modules included in your system for more details...

Page 28: ...or set to tubing you must mark the compression screw and connection port and ensure that the two marks line up when you tighten them Warning To avoid eye injury use eye protection when performing this...

Page 29: ...se 3 4 turn until the two marks line up First use tightening 2 7 1 2 Stainless steel gold plated fitting with long flats and 2 piece stainless steel ferrule V detail First use Long flats Compression s...

Page 30: ...led Long flats Compression screw 2 piece stainless steel ferrule Tighten the fitting finger tight plus as much as an additional 1 6 turn using a 1 4 inch open end wrench Reinstalled tightening April 4...

Page 31: ...ghten the fitting finger tight plus an additional 3 4 turn using a 1 4 inch open end wrench For detailed instructions about assembling new fittings see Assembling new fittings Tip To prevent band spre...

Page 32: ...g finger tight plus as much as an additional 1 6 turn using a 1 4 inch open end wrench Reinstalled tightening 2 7 1 4 1 4 28 flangeless fitting with ferrule First use or re installed 1 Compression scr...

Page 33: ...en the fitting finger tight 2 7 1 6 Long 1 4 28 fitting with flangeless ferrule and stainless steel lock ring installed on 1 8 inch OD tubing First use or re installed Compression screw Lock ring Ferr...

Page 34: ...w Lock ring Ferrule End of lock ring with larger inside diameter ID Tighten the fitting finger tight 2 7 1 8 5 16 24 fitting with filter and stainless steel lock ring First use or re installed Compres...

Page 35: ...rst use or reinstalled Compression screw Tighten the fitting finger tight Tips You can also use the column gripping tool when tightening this fitting To prevent band spreading ensure that the tubing i...

Page 36: ...ed 1 knurled compression fitting 2 locking cap nut Back locking PEEK ferrule Figure 2 3 Column stabilizer outlet fitting first use or reinstalled 1 hex compression fitting 2 locking cap nut Back locki...

Page 37: ...e in line filter Important To prevent band spreading ensure that the tubing bottoms in the connection port before you tighten the compression fitting 3 Finger tighten the compression fitting into the...

Page 38: ...et ferrule on the APH assembly fitting if the column is leaking or the ferrule looks damaged Warning To prevent burn injuries set the column temperature to Off and then allow the column compartment an...

Page 39: ...mpression fitting off the tubing Figure 2 7 Sliding fitting off tubing 1 knurled compression fitting Tubing Note If the assembly contains a captive ferrule the ferrule remains locked in the fitting 3...

Page 40: ...ule on the fitting 6 Slide the 1 knurled compression fitting and ferrule onto the tubing Figure 2 9 Sliding fitting and ferrule onto tubing Tubing 1 knurled compression fitting Note If it is a captive...

Page 41: ...ons on choosing and handling solvents 2 8 2 Matching sample diluents When you use the auto dilution option on the SM FTN the purge solvent serves as the sample diluent Ensure that your sample solution...

Page 42: ...g start up tests run CPU board memory RAM and ROM external communication system Ethernet and clock If the start up tests indicate a malfunction contact your local Waters representative 2 Power on the...

Page 43: ...select Run Samples Result A control panel for each device in the system appears 2 In the sample manager control panel click Display console Alternative Right click the control panel for any module and...

Page 44: ...he following start up tests run CPU board memory RAM and ROM external communication system Ethernet and clock If the start up tests indicate a malfunction contact your local Waters representative 2 Po...

Page 45: ...ly even when the power switch is in the off position These fans switch off only when you disconnect the power cable from the ac wall outlet or rear panel 3 3 4 Status LEDs 3 3 4 1 Flow LED Flow status...

Page 46: ...r is operating normally completing any outstanding samples or diagnostic function requests Flashing green The sample manager is initializing Flashing red An error stopped the sample manager Refer to t...

Page 47: ...tus unless previously enabled To enable the leak sensors 1 In the ACQUITY UPLC Console select Control Leak Sensors 2 To enable the leak sensor for an individual module click the status on the left han...

Page 48: ...e all solvents and to specify the solvent composition flow rate column and sample temperatures and needle characterization for your next system start up For details see the console online Help 3 5 Mon...

Page 49: ...values range from 0 0 to 100 0 Stop flow Immediately stops all flow from the binary solvent manager Pressure Delta The difference between the maximum and minimum pressures observed during the previous...

Page 50: ...can edit these values when the system is idle by clicking the underlined value You cannot edit sample manager set points while the system is running samples Tip To keep the sample compartment at a co...

Page 51: ...e sample manager control panel Control panel function Description Prime syringes Displays the Prime dialog box Wash needle Displays the Wash Needle dialog box Turn lights on Illuminates the ACQUITY UP...

Page 52: ...anel Table 3 6 Additional functions in the TUV detector control panel Control panel function Description Auto zero Resets the absorbance value to 0 Reset TUV Resets the detector after an error conditi...

Page 53: ...ys the current column compartment temperature Temperature set point Displays the column compartment set point When active temperature control is disabled this field displays Off You can access these a...

Page 54: ...em modules Connect the shielded Ethernet cables from each module to the electronic connections on the rear panel of the sample manager The sample manager is connected internally to the Ethernet switch...

Page 55: ...ed off 2 Connect the external communication cable to the High Density HD port on the rear of the column heater 3 Connect the other end of the external communication cable to the PSPI port on the rear...

Page 56: ...A 3 External cable connections Figure A 1 System rear panel cable connections April 4 2018 715005704 Rev A Page 56...

Page 57: ...r Sample manager Solvent manager Sample organizer Ethernet switch Workstation Interconnect D sub cables Ethernet cables A 4 Plumbing connections Figure A 2 System plumbing connections April 4 2018 715...

Page 58: ...Detector Column heater Sample manager Solvent manager Sample organizer Stainless steel tubing PEEK tubing April 4 2018 715005704 Rev A Page 58...

Page 59: ...A 5 Waste tubing connections Figure A 3 System waste tubing connections April 4 2018 715005704 Rev A Page 59...

Page 60: ...erforming any maintenance operation on it Connect each module to a common ground Note Some column modules such as the column heater CH A the column heater 30 cm CH 30A and the column manager auxiliary...

Page 61: ...driver Flat blade screwdriver Connector Signal cable To connect the cables 1 Insert the connector into the connector port on the module s rear panel Figure A 4 Inserting connector into connector port...

Page 62: ...minal on the rear panel grounding stud and secure the terminal using the locking nut Note Use the 9 32 inch nut driver to tighten the locking nut until the terminal does not move Figure A 6 Grounding...

Page 63: ...r I O signal cables These connectors are keyed so that they can be inserted only one way Figure A 7 Binary solvent manager I O signal connectors Connector 1 Auxiliary 1 In Auxiliary 1 In Auxiliary 2 I...

Page 64: ...2V Analog 2 Out 0 2V Analog 2 Out For electrical specifications see the Binary Solvent Manager Overview and Maintenance Guide on your documentation media or at Waters com for more details Table A 1 Bi...

Page 65: ...ware failure occurs on another system module Switch 2 Out Used to send time based contact closure signals to external devices Switch 3 Out Used to send time based contact closure signals to external d...

Page 66: ...Inject Start Out Indicates with a contact closure output that an injection has started Inject Hold In Delays the next injection when the sample manager receives a contact closure input from another sy...

Page 67: ...er supplies to operate To connect the column module Notice To avoid damaging the electronic components of the sample manager and the column heater or column heater cooler always power off the sample m...

Page 68: ...liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing repairing or operating any of its instruments or devices...

Page 69: ...gical agents that can pose a serious health threat Warning Risk of tipping Warning Risk of explosion Warning Risk of high pressure gas release B 1 1 Specific warnings The following warnings both symbo...

Page 70: ...0 psi during an analysis requiring the use of flammable solvents Also a gas fail device must be installed to interrupt the flow of LC solvent should the nitrogen supply fail B 1 1 3 Mass spectrometer...

Page 71: ...st edition of the National Research Council s publication Prudent Practices in the Laboratory Handling and Management of Chemical Hazards Observe Good Laboratory Practice GLP at all times particularly...

Page 72: ...iritto all utilizzo dell apparecchiatura da parte dell utente Advertencia cualquier cambio o modificaci n efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del c...

Page 73: ...lle offenen Flammen in der N he l schen Keine Schl uche verwenden die stark geknickt oder berbeansprucht sind Nichtmetallische Schl uche nicht f r Tetrahydrofuran THF oder konzentrierte Salpeter oder...

Page 74: ...trabajar con tubos que se hayan doblado o sometido a altas presiones Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano THF o cidos n trico o sulf rico concentrados Hay que t...

Page 75: ...tion assur e par le mat riel risque d tre d fectueuses Warnung Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwendung des Ger tes die eingebauten Sicherheitseinrichtungen unter U...

Page 76: ...Electrical power off Standby Direct current Alternating current Alternating current 3 phase Safety ground Frame or chassis terminal connection Fuse Functional ground Input Output B 4 2 Handling symbo...

Page 77: ...with interlocking features Warning To avoid spinal and muscular injury do not attempt to lift a system module without assistance Warning To avoid crushing your fingers beneath or between modules use...

Page 78: ...e remaining system modules B 6 Stacking system modules without interlocking features This procedure applies to system modules that are not equipped with interlocking features Most modules will however...

Page 79: ...e 2 Carefully lower the module so that the feet rest in the indents Important To maintain the integrity of the system stack and integrated waste system ensure that the feet of the upper module rest in...

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