Waters ACQUITY UPLC I-Class IVD System Manual Download Page 1

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ACQUITY UPLC

I-Class IVD

System Guide

715003736IVD / Revision C

Summary of Contents for ACQUITY UPLC I-Class IVD

Page 2: ...ii July 7 2014 715003736IVD Rev C...

Page 3: ...r any errors that may appear in this document This document is believed to be complete and accurate at the time of publication In no event shall Waters Corporation be liable for incidental or conseque...

Page 4: ...f You must know the potentially hazardous effects of all substances you work with Always follow good laboratory practice and consult your organization s standard operating procedures Waters contact in...

Page 5: ...any interference received including interference that may cause undesired operation Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES 001 C...

Page 6: ...visories Consult Appendix A for a comprehensive list of warning and caution advisories Operating the ACQUITY UPLC I Class IVD system When operating the ACQUITY UPLC I Class IVD system follow standard...

Page 7: ...ting To calibrate LC systems follow acceptable calibration methods using at least five standards to generate a standard curve The concentration range for Confirms that a manufactured product complies...

Page 8: ...ed or regulatory thresholds See the TargetLynx Online help topic QC Monitor properties Data should also be manually inspected prior to the reporting of any results When analyzing samples from a comple...

Page 9: ...generated and or used conductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class B products are suitable for use in both commercial and resident...

Page 10: ...x July 7 2014 715003736IVD Rev C...

Page 11: ...nt misuse notice vi Safety advisories vi Operating the ACQUITY UPLC I Class IVD system vi Applicable symbols vi Audience and purpose vii Intended use of the ACQUITY UPLC I Class IVD system vii Calibra...

Page 12: ...y 26 Cycle time between injections 26 Preventing leaks 27 Sample preparation 28 Particulates 28 Matching sample diluents 28 3 Preparing the System 29 Preparing system hardware 29 Powering on the syste...

Page 13: ...ings 51 Caution advisory 53 Warnings that apply to all Waters instruments 53 Electrical and handling symbols 59 Electrical symbols 59 Handling symbols 60 B Specifications 61 Physical specifications 61...

Page 14: ...ricity source 78 D Solvent Considerations 81 Solvent guidelines 81 Clean solvents 81 Solvent quality 82 Solvent preparation 82 Water 82 Solvent recommendations 83 Recommended solvents 83 Solvents to a...

Page 15: ...y 7 2014 715003736IVD Rev C xv Solvent viscosity 90 E FlexCart Assembly 91 Assembling the FlexCart 91 Adjusting the FlexCart s height 93 Locking the FlexCart in place 94 Moving the assembled FlexCart...

Page 16: ...xvi July 7 2014 715003736IVD Rev C...

Page 17: ...compounds in biological sample matrices The system is intended to be used by only highly qualified operators System components Major components of the ACQUITY UPLC I Class IVD System The ACQUITY UPLC...

Page 18: ...r The Waters ACQUITY UPLC I Class IVD Binary Solvent Manager BSM delivers solvent compositions for isocratic and binary gradient methods at flow rates of between 0 01 and 2 0 mL per minute Its feature...

Page 19: ...lass IVD column heater CH A controls the column temperature independent of flow rate The column heater CH A heats the column compartment to any temperature between 20 C and 90 C that is at least 5 C a...

Page 20: ...s run on the column The eCord column chip provides documentation of the column used for each chromatographic run and records the following information Number of injections onto the column Number of sa...

Page 21: ...or performance run diagnostic tests and maintain the system and its modules Connections INSIGHT software monitors the system s operational characteristics It securely transmits system usage performanc...

Page 22: ...CD you can find this additional information about the ACQUITY UPLC I Class IVD system 715003740IVD ACQUITY UPLC I Class IVD Binary Solvent Manager Overview and Maintenance Guide part number 715003739I...

Page 23: ...n Moreover analysis time for UPLC is shorter and solvent and sample consumption are significantly reduced The ACQUITY UPLC I Class chromatograph requires optimum performance from the sample manager be...

Page 24: ...filters on tubing lines in solvent bottles Filter buffers with a 0 2 m filter membrane Keep concentrated stock solutions to use when preparing working solutions Do not add fresh buffer to old which ca...

Page 25: ...bserve carryover in chromatographic systems when a previously injected analyte appears as a peak in the chromatogram of subsequent samples Carryover tends to occur when a small amount of analyte remai...

Page 26: ...ing carryover problems Contamination To minimize contamination ensure cleanliness during sample preparation For example verify that sample preparation tools are cleaned thoroughly See also Controlling...

Page 27: ...sis Leaks can potentially occur at any tubing connection gasket or seal but are most common at tubing connections Low pressure leaks on the intake side of the solvent manager s pump cause solvent loss...

Page 28: ...ents and sample solutions are therefore essential for UPLC analysis See General guidelines on page 23 for recommendations on choosing and handling solvents Matching sample diluents When you use the au...

Page 29: ...us LEDs on page 30 and Power LED on page 30 for information on how to interpret indicator LED modes for device or instrument flow status and whether the units are powered on Tip The column heater is a...

Page 30: ...s state of functioning The LEDs are specific to their modules so the significance of their various colors and modes can differ from one module to another Power LED The power LED on the left hand side...

Page 31: ...ger Indicates the device is currently idle Steady green Binary solvent manager Indicates solvent is flowing Sample manager Indicates the sample manager is operating normally attempting to complete any...

Page 32: ...m has been idle for four or more hours and you intend to use the solvents already in the system Prime the binary solvent manager for four minutes for each solvent when you intend to use fresh solvents...

Page 33: ...ls ACQUITY control panels You can monitor control panels for the binary solvent manager sample manager and detector from MassLynx software The control panels appear on the Additional Status tab of the...

Page 34: ...nager unless communications with the binary solvent manager are lost Status Displays the status of the current operation System Pressure Displays system pressure in kPa bar or psi You can customize pr...

Page 35: ...er Operator s Overview and Maintenance Information Prime seal wash Starts priming the seal wash See Priming the seal wash system in the Binary Solvent Manager Operator s Overview and Maintenance Infor...

Page 36: ...The following table describes the items in the sample manager s control panel Sample manager control panel items Control panel item Description Run LED Displays the actual run LED on the front panel...

Page 37: ...onsole Displays the ACQUITY UPLC Console Additional functions in the sample manager control panel Control panel function Description Prime Displays the Prime dialog box See Priming the SM FTN in the S...

Page 38: ...ITY UPLC Console functions independently of data applications and does not recognize or control them From the ACQUITY UPLC Console s interface you can quickly navigate to visual representations of eac...

Page 39: ...se When using the system throughout the day and on nights and weekends or when using aggressive solvents such as buffers perform these maintenance tasks more frequently See also For complete procedure...

Page 40: ...pical failure modes are as follows Error message Any active preheater error messages including messages indicating the active preheater is not detected or connected or has a temperature sensor error L...

Page 41: ...n in line filter unit As needed The typical failure modes are as follows High back pressure The column in line causes a high pressure shutdown In this case disconnect the in line filter If the system...

Page 42: ...needle seal readiness test fails Peaks do not appear on your chromatogram Peak areas are lower than expected Replace the sample needle Replace when the needle breaks when the error message Drawing sa...

Page 43: ...wn Replace the pump interconnect tubing Replace as part of the annual scheduled routine maintenance and thereafter as needed The typical failure modes are as follows You see a visible leak The system...

Page 44: ...needed The typical failure modes are as follows Late eluting peaks are exhibited and or after a dynamic leak test was performed where the leak rate is above the acceptable limit The solvent manager e...

Page 45: ...h pressure dynamic leak test The solvent manager exhibits low or erratic flow rate pressure pulsations Replace the vent valve cartridge Replace as part of your annual routine scheduled maintenance and...

Page 46: ...ust install its service agent software on your MassLynx workstation In a client server system you must also install the service agent on the computer from which you control the system The service agen...

Page 47: ...void personal contamination with biohazards toxic compounds or their residues wear clean chemical resistant powder free gloves when handling the components Warning To prevent injury always observe Goo...

Page 48: ...e status information that can help you determine when to schedule routine maintenance for specific components You can set usage thresholds and maintenance warnings that alert you when a component reac...

Page 49: ...of death injury or seriously adverse physiological reactions associated with an instrument s use or misuse Heed all warnings when you install repair and operate Waters instruments Waters assumes no l...

Page 50: ...nformation before you use the instrument Warning Risk of hot surfaces Warning Risk of electric shock Warning Risk of fire Warning Risk of sharp point puncture injury Warning Moving machinery Warning R...

Page 51: ...d or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran THF and nitric or sulfuric acids Be aware that some compounds like methylene chloride and dimethyl sulfoxide...

Page 52: ...be used to analyze or process potentially infectious human sourced products inactivated microorganisms and other biological materials To avoid infection with these agents assume that all biological fl...

Page 53: ...g it or compromising a sample s integrity The exclamation point symbol and its associated statement alert you to such risk Warnings that apply to all Waters instruments When operating this device foll...

Page 54: ...ede nderungen oder Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Sy...

Page 55: ...toute flamme se trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide...

Page 56: ...provocano rigonfiamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a p...

Page 57: ...Warnings that apply to all Waters instruments July 7 2014 715003736IVD Rev C 57 Nonmetallic Tetrahydrofuran THF Methylene chloride Dimethyl sulfoxide THF...

Page 58: ...sque d tre d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes die eingebauten Sicherheitseinrichtungen unter Umst nden nicht ordnungsgem fu...

Page 59: ...d handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternati...

Page 60: ...2014 715003736IVD Rev C Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright...

Page 61: ...ectrometry System Operator s Overview and Maintenance Guide Physical specifications Binary solvent manager The following table lists the physical specifications for the ACQUITY UPLC I Class IVD BSM Co...

Page 62: ...he physical specifications for the ACQUITY UPLC I Class IVD column heater A Sample manager FTN physical specifications Attribute Specification Height 27 1 cm 10 7 inches Width 34 3 cm 13 5 inches Dept...

Page 63: ...hes Weight with two scroll pumps 352 kg 777 pounds Weight with two rotary pumps 408 kg 899 pounds Warning To prevent inaccurate results do not operate the instrument outside the operating temperature...

Page 64: ...plug Class I Overvoltage category2 2 Overvoltage Category II Pertains to instruments that receive their electrical power from a local level such as an electrical wall outlet II Pollution degree3 3 Po...

Page 65: ...s Screw terminal connector Run stopped output Maximum voltage 30 VDC Maximum current 0 5 A Maximum VA rating 10 W Contact resistance nominal 0 2 ohms Screw terminal connector Behavior If an error mess...

Page 66: ...re Input voltage range 30 VDC Logic High 3 0 VDC Logic Low 1 9 VDC Minimum pulse width 100 msec Screw terminal connector Analog outputs 1 and 2 0 to 2 volts full scale screw terminal digital to analog...

Page 67: ...or the sample ACQUITY UPLC I Class IVD SM FTN Xevo TQ S input output specifications Attribute Specification Xevo TQ S 200 240 V 50 60 Hz 2 kW maximum XDS 46i scroll pump 200 230 V 50 60 Hz XDS 100B sc...

Page 68: ...03736IVD Rev C Column Heater The following table lists the wetted materials of construction for the ACQUITY UPLC I Class IVD column heater Wetted materials of construction Description Specification We...

Page 69: ...Caution To avoid damaging system modules Contact Waters Technical Service see page iv If you must transport a system component or remove it from service contact Waters Technical Service for recommende...

Page 70: ...hand side of the BSM SM FTN and CH Solvent tray Mass spectrometer Divert Valve Sample Manager FTN BSM solvents SM wash solvent Column Column heater APH Needle To waste Injector valve Vent valve Mixer...

Page 71: ...trument external wiring connections The rear panel connections for ACQUITY UPLC I Class IVD system modules are shown below TP03533 Power cords Ethernet cables Solvent tray Xevo TQ S Column heater Exte...

Page 72: ...500074403 Column heater connection The sample manager powers and communicates with the column heater The external communication cable must be connected to the rear of the column heater and the sample...

Page 73: ...connection location shown on the silk screened label affixed to the rear panel of each system module Required materials 9 32 inch nut driver Flat blade screwdriver Connector Signal cable To connect si...

Page 74: ...ds of the signal cable to the connector 3 Fit the grounding cable s fork terminal on the rear panel grounding stud and secure the terminal with the locking nut Tip Use the 9 32 inch nut driver to tigh...

Page 75: ...y 1 In Reserved for future use Auxiliary 2 In Reserved for future use Run Stopped Out Indicates with a contact closure the binary solvent manager has ceased operation because of an error condition or...

Page 76: ...to stop the flow from the binary solvent manager when an error condition or hardware failure occurs on another system module Switch 2 Out Used to send time based contact closure signals to external de...

Page 77: ...osure output connection Inject Start Out from the sample manager is required to trigger a mass spectrometer Sample manager I O signal connectors For electrical specifications see Appendix B Chart out...

Page 78: ...end of the FlexCart s electrical cables included in the startup kit to the receptacle on the rear panel of each system module Connect the hooded male end of the FlexCart s electrical cables to the po...

Page 79: ...power connections optional TP02578 To circuit A To circuit B AC line AC line Network switch if needed Sample manager Binary solvent manager LCD monitor Computer workstation FlexCart power strips All c...

Page 80: ...C External Connections 80 July 7 2014 715003736IVD Rev C...

Page 81: ...ning To avoid injury familiarize yourself with materials and their hazards observe Good Laboratory Practice GLP and consult your organization s safety representative regarding proper use and handling...

Page 82: ...s in borosilicate glass reservoirs type 1 class A2 or type 3 33 Use brown stained glassware to inhibit microbial growth Use aluminum foil or Waters caps to cover the reservoirs Water Use water only fr...

Page 83: ...IVD system was designed for reversed phase chromatography and ACQUITY UPLC BEH column chemistries Waters evaluated the system s reliability using traditional reversed phase solvents This section lists...

Page 84: ...rm peroxides such as UV grade ethers dioxane and diisopropylether Recommended additives modifiers 0 3 vol acetic acid 50 mM ammonium acetate 10 mM ammonium bicarbonate 50 mM ammonium hydroxide 0 1 wt...

Page 85: ...tonitrile Acetonitrile water mixtures Chloroform Dimethylformamide DMF Dimethyl sulfoxide DMSO Isooctane Isopropanol Methanol Methanol water mixtures Methylene chloride Water Recommended cleaning agen...

Page 86: ...systems Always use properly cleaned and maintained glassware Binary solvent manager recommendations The seal wash system must never run dry particularly during separations that use a polar mobile pha...

Page 87: ...rile 88 8 at 25 C 81 6 6 n butyl acetate 7 8 at 20 C 126 11 22 n butyl alcohol 4 4 at 20 C 117 5 37 n butyl chloride 80 1 at 20 C 78 44 9 Chlorobenzene 8 8 at 20 C 131 69 28 Chloroform 158 4 at 20 C 6...

Page 88: ...sobutyl alcohol 8 8 at 20 C 107 7 28 Isopropyl alcohol 32 4 at 20 C 82 26 12 Isopropyl myristate 1 at 20 C 192 6 164 Methanol 97 at 20 C 64 7 11 Methyl t butyl ether 240 at 20 C 55 2 28 Methyl ethyl k...

Page 89: ...fference between the two M numbers is 15 or less the two liquids are miscible in all proportions at 15 C A difference of 16 indicates a critical solution temperature from 25 to 75 C with 50 C as the o...

Page 90: ...lvents and have M numbers of 0 and 32 Two liquids with dual M numbers are usually miscible with each other A liquid is classified in the M number system by testing for miscibility with a sequence of s...

Page 91: ...you assemble the instruments and components on the FlexCart To assemble the FlexCart 1 Remove the contents from the box packaged in the FlexCart Tip The box contains the monitor arm the bolts used to...

Page 92: ...plate at the rear of the monitor and mount the monitor onto the movable arm assembly Refer to the IBM instruction book if necessary Top tray Power connectors 2 Monitor mounting bracket Shelf mounting...

Page 93: ...plug them into the monitor Refer to the IBM instruction book if necessary 6 Insert the keyboard shelf s 2 captive thumbscrews into slots on the cart s front panel at a level that affords comfortable a...

Page 94: ...ing needed between the instruments stacked on the cart and the mass spectrometer 4 Tighten the side lock knobs after reaching the desired height 5 Loosen the keyboard shelf s 2 captive thumbscrews on...

Page 95: ...areas of a lab to minimize tubing runs between the ACQUITY UPLC instruments and a mass spectrometer Use the lip on top of the cart to pull it Caution To avoid spills of damaging solvent remove all so...

Page 96: ...E FlexCart Assembly 96 July 7 2014 715003736IVD Rev C...

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