Maintaining the detector
45
Tip:
Conditions other than a dirty flow cell can cause decreased lamp
intensity. For more information, refer to
Flow cell maintenance consists of
•
flushing the flow cell.
•
removing the flow cell.
•
installing the flow cell assembly.
Flushing the flow cell
Required Materials
•
UPLC-grade solvent
•
UPLC-grade methanol
If the flow cell requires cleaning, first try flushing it with solvent.
To flush the flow cell:
1. Select a solvent compatible with the samples and mobile phases that you
have been using. If you have been using buffers, flush with 10 mL of
UPLC-grade solvent, then flush with 10 mL of a low-surface-tension
solvent such as methanol.
Tip:
Ensure that the solvent is miscible with the previous mobile phase.
2. Stop the solvent flow to vent the system:
a. From the console, select Binary Solvent Manager in the system tree.
b. Enter zero flow in the composition field or press the Stop Flow icon
and enter a flow rate of zero. The system will vent to atmospheric
pressure through the vent valve in the ACQUITY UPC
2
Convergence Manager.
Warning:
To avoid burn injuries resulting from skin contact with
pressurized, liquid CO
2
and breathing difficulties caused by the
displacement of oxygen within the confines of the laboratory space,
stop the solvent flow, and vent the system pressure before you
attempt flush the flow cell.
Summary of Contents for ACQUITY UPC2
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