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ACQUITY UPC

System Guide

715004521
Revision C

2016

Summary of Contents for ACQUITY UPC2

Page 2: ...November 16 2016 715004521 Rev C Page ii...

Page 3: ...THE SCIENCE OF WHAT S POSSIBLE ACQUITY ACQUITY UPC 2 ACQUITY UPLC UPLC Connections INSIGHT Waters Quality Parts Empower MassLynx Viridis and UPC 2 are registered trademarks of Waters Corporation and...

Page 4: ...lted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken Considerations specific to the UPC2 system Power cord replacement hazar...

Page 5: ...ubject to the following two conditions 1 this device may not cause harmful Warning To avoid crushing your fingers beneath or between modules use extreme care when installing a module in the system sta...

Page 6: ...be rendered ineffective Safety advisories Consult Appendix A for a comprehensive list of warning advisories and notices Operating this system When operating this system follow standard quality contro...

Page 7: ...e normal levels of a compound If sample trays are the same or very similar vary the location of the QC samples in the trays Ensure that QC sample results fall within an acceptable range and evaluate p...

Page 8: ...nerated and or used conductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class B products are suitable for use in both commercial and residential...

Page 9: ...rol vii EMC considerations vii Canada spectrum management emissions notice vii ISM Classification ISM Group 1 Class B viii EC authorized representative viii 1 ACQUITY UPC2 system 13 1 1 Using ACQUITY...

Page 10: ...ings 35 3 2 2 SM FL instrument method settings 36 3 2 3 Instrument method settings for the column manager 36 3 2 4 Instrument method settings for the ccManager 36 3 2 5 ACQUITY UPC2 PDA instrument met...

Page 11: ...e ACQUITY UPC2 system modules 77 6 2 Maintaining the ABPR 77 6 2 1 Running an ABPR health test when the system includes a CM 30S 77 6 3 Spare parts 79 6 4 Troubleshooting with Connections INSIGHT 79 6...

Page 12: ...ith active preheating and auxiliary column manager specifications 99 B 7 ACQUITY 30 cm single zone column manager specifications 100 B 8 ACQUITY UPC2 Photodiode array detector specifications 101 B 9 S...

Page 13: ...se advantages over traditional HPLC normal phase chromatography Uses liquid CO2 a chemically pure stable and nonpolar solvent as the primary mobile phase Combines liquid CO2 with one or more organic s...

Page 14: ...the critical point they are highly tunable solvents Recommendation Use only food grade or better house supplied CO2 or non siphon CO2 at a flow pressure of 6 205 to 7 584 kPa 62 to 76 bar 900 to 1100...

Page 15: ...system stack configurations and supported modules Figure 1 2 Core 1 configuration 2 column system Figure 1 3 Core 2 configuration 4 column system 73 PDA CM A ccManager SM FL ccBSM Side mount solvent...

Page 16: ...column system See also Appendix C for information about configuring a system with two CM 30S modules 1 2 2 Major components of the ACQUITY UPC2 system The following table describes system modules and...

Page 17: ...ndently heated or cooled column troughs for inlet configuration on the left hand or right hand side Each column position includes an active pre heater for both configurations to ensure specified therm...

Page 18: ...TY UPC 2 system because it can burst in the event of over pressure conditions Notice To avoid fluid backup ensure proper drainage of waste Place the waste container below the system stack Ensure that...

Page 19: ...November 16 2016 715004521 Rev C Page 19 Figure 1 6 Solvent flow path through a Core 1 system...

Page 20: ...November 16 2016 715004521 Rev C Page 20...

Page 21: ...eans of the console user interface 2 1 1 Before you power on the system modules Verify that all fittings are properly tightened to prevent leaks Preventing leaks ensures that the system maintains adeq...

Page 22: ...e LED indicator on the right hand side on each module to determine its operational status Requirement If communication with system modules is interrupted you must reset the software To do so right cli...

Page 23: ...entations of each module and their components You can start the console from within the data system that you are using See also The Empower software or MassLynx software online Help for details about...

Page 24: ...are using In Empower software in the Run Samples window click Display console or right click and select a menu item In MassLynx software Inlet Method on the Additional Status tab click Display consol...

Page 25: ...ing process runs solvent through the lines to flush any particulates 2 3 1 Priming the system Always prime the system under these conditions After replacing the needle When the system has been idle fo...

Page 26: ...equence The following diagrams illustrate the sample set up and injection sequence in an ACQUITY UPC2 system Figure 2 3 Step 1 SM FL runs internal setup tests and decompresses the sample loop At the s...

Page 27: ...the vial the programmed amount of sample from the vial post sample buffer from the vial and then returns the needle to above the injection port aspirating a final air gap while in that position Auxil...

Page 28: ...on port the metering syringe pulls the sample aliquot past port 2 of the injection valve and into the VDD tubing Waste Waste Auxiliary valve load position Metering syringe Injection valve inject posit...

Page 29: ...alve rotates into the load position The metering syringe loads the programmed sample volume into the sample loop Waste Waste Auxiliary valve load position Metering syringe Injection valve Load positio...

Page 30: ...nject position and places sample in line with the connecting tubes between the auxiliary valve and the injection valve Waste Waste Auxiliary valve load position Metering syringe Injection valve inject...

Page 31: ...ows mobile phase to pass through the injection valve This introduces sample into the high pressure liquid CO2 system and injects the sample onto the column Waste Waste Auxiliary valve inject position...

Page 32: ...for the compounds you want to separate A typical column screening workflow involves these actions Create a method for each column that you want to screen In each method create a gradient consisting o...

Page 33: ...gthen lamp life extinguish the detector lamp by clicking Lamp Off in the detector control panel Instruction If you are running under MassLynx control ensure that Auto Shutdown for your shutdown method...

Page 34: ...ts temperature to 40 C Create a system shutdown method Before using any system or instruments that have been shut down under the recommended conditions ensure that the new mobile phase is miscible wit...

Page 35: ...ftware online Help the topic Using a quick start guided tour to learn about Empower Click on the Empower Quick Start Tour link Also see the topics in the Define Methods section in the Empower software...

Page 36: ...ty problems and split peaks Dilute samples in heptane or similar weak solvents instead of in methanol You can use as much as 20 methanol with heptane Do not use water as the sample diluent or wash sol...

Page 37: ...id CO2 which is similar to setting up a solvent gradient to increase solvent strength Figure 3 1 Pressure gradient example When setting pressure gradients observe these guidelines Higher ABPR pressure...

Page 38: ...ck the Instrument area in the MassLynx main window Use the Configuration Wizard to select the pump autosampler and detector that you are using and then specify settings for acquiring data Notes When s...

Page 39: ...t before moving the ACQUITY UPC2 system modules If you must transport a system component or remove it from service contact Waters Technical Service department for recommended cleaning flushing and pac...

Page 40: ...system modules Connect the shielded Ethernet cables from each module to the electronic connections on the rear panel of the SM FL The SM FL is connected internally to the Ethernet switch Requirement I...

Page 41: ...les 1 Using the flat blade screwdriver attach the positive and negative leads for the channels of interest to the connector 2 Insert the connector into the connector port on the back of the system mod...

Page 42: ...they can be inserted only one way Figure 4 2 ccBSM analog out event in signal connectors Fork terminal Locking nut Grounding stud Connector I Connector II 1 2 3 4 5 6 7 8 9 10 11 12 Auxiliary 1 In Aux...

Page 43: ...e the ccBSM stopped operating because of an error condition or operator request Switch 1 Out Reserved for future use 0 2V Analog 1 Out Reserved for future use Gradient In Initiates the pumps to begin...

Page 44: ...grounded wall outlet Alternative If your system includes the optional FlexCart connect the female end of the FlexCart s electrical cables included in the startup kit to the receptacle on the rear pane...

Page 45: ...er connections To circuit A To circuit B 1 meter 2 meters 1 meter 1 meter 1 meter 1 meter 2 meters Universal IEC coupler AC line AC line Network switch if needed PDA SM FL ccBSM ccManager LCD monitor...

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Page 47: ...alve outlet port You do not use the 515 makeup pump kit s restrictor tube and union for this configuration The MS splitter assembly is shipped with the 29 5 inch PEEKsil tubing pre connected to the ex...

Page 48: ...o the 8 inch makeup pump restrictor tube 1 2 3 Bracket Alternative Remove this tubing to connect a tool free ECI or APCI probe capillary from the mass spectrometer 29 5 inch x 50 m ID 50 inch x 010 in...

Page 49: ...PEEK 29 5 inch x 50 m ID 46 0 inch x 007 inch ID 50 0 inch x 010 inch ID To the ccManager To the ELSD From the CM 30S or PDA Bracket 1 2 3 46 inch x 007 inch ID From the CM 30S or PDA 4 29 5 inch x 5...

Page 50: ...on or triple detection flow splitter kit See also The ACQUITY QDa Detector Operator s Overview and Maintenance Guide for more information on how to configure your QDa detector The ACQUITY Isocratic So...

Page 51: ...r dual detection Figure 5 8 ISM splitter module for triple detection From the PDA To the ccManager QDa detector 0 010 inch ID PEEK 0 007 inch ID PEEK 1 2 From the PDA To the ccManager 0 010 inch ID PE...

Page 52: ...t mount locations Figure 5 9 Splitter bracket mount location on the SM FL Figure 5 10 Splitter bracket mount location on the CM A TP03510 ccManager SM FL Bottle tray bracket Splitter bracket TP03511 c...

Page 53: ...er 16 2016 715004521 Rev C Page 53 Figure 5 11 Splitter bracket mount location on the CM AUX Figure 5 12 Splitter bracket mount location on the CM 30S TP03508 Splitter bracket CM Aux Splitter bracket...

Page 54: ...diagrams and tubing connections Note The figures in this section describe recommended splitter bracket mounting locations If you choose other locations to mount the splitter assembly bracket ensure th...

Page 55: ...escription From To ID Notes recommended trim length 1 29 5 inch PEEKsil tubing Splitter module ELSD 50 m Do not trim 2 46 inch PEEK tubing pre connected to the splitter CM A or PDA Splitter module 0 0...

Page 56: ...ion with a PDA SQD and splitter The following table describes final tubing connections and does not list factory installed tubing connections TP03524 PDA CM A MS splitter mounted to CM A Solvent tray...

Page 57: ...r module SQD probe 50 m Do not trim 2 50 inch PEEK tubing pre connected to the splitter Splitter module Left side of the ccManager tee 0 010 inches Do not trim more than 23 inches 3 59 inch PEEK tubin...

Page 58: ...ation with a PDA TQ XS and splitter The following table describes final tubing connections and does not list factory installed tubing connections PDA CM A MS splitter mounted to CM A Solvent tray TQ X...

Page 59: ...inch PEEKsil tubing that is pre connected to the module s external V detail fitting and connect the probe capillary in its place TQ XS inlet probe Splitter module 50 m Do not trim 2 50 inch PEEK tubin...

Page 60: ...on with a PDA ISM and QDa Tube Tube description From To ID Notes recommended trim length 1 60 0 inch PEEK tubing ISM splitter module ccManager vent valve tee 0 007 inches Optional trim up to 30 inches...

Page 61: ...Tube Tube description From To ID Notes recommended trim length 1 60 0 inches PEEK tubing ISM splitter module ccManager vent valve tee 0 007 inches Optional trim up to 30 inches 2 28 inches PEEK tubing...

Page 62: ...connections Figure 5 20 Core 2 configuration with a PDA ELSD and splitter The following table describes final tubing connections and does not list factory installed tubing connections TP03524 SM FL cc...

Page 63: ...length 1 29 5 inch PEEKsil tubing pre connected to the splitter Splitter module ELSD 50 m Do not trim 2 46 inch PEEK tubing pre connected to the splitter CM A or PDA Splitter module 0 007 inches Do n...

Page 64: ...ctro meter 50 m Do not trim 2 50 inch PEEK tubing pre connected to the splitter Splitter module Left side of the ccManager tee 0 010 inches Do not trim more than 23 inches 3 59 inch PEEK tubing pre co...

Page 65: ...nections Figure 5 22 Core 3 configuration with a PDA ELSD and splitter The following table describes final tubing connections and does not list factory installed tubing connections TP03530 ELSD PDA CM...

Page 66: ...ngth 1 29 5 inch PEEKsil tubing pre connected to the splitter Splitter module ELSD 50 m Do not trim 2 46 inch PEEK tubing pre connected to the splitter CM A or PDA Splitter module 0 007 inches Do not...

Page 67: ...pectrometer 50 m Do not trim 2 50 inch PEEK tubing pre connected to the splitter Splitter module Left side of the ccManager tee 0 010 inches Do not trim more that 8 inches 3 59 inch PEEK tubing pre co...

Page 68: ...PDA ISM and QDa Tube Tube description From To ID Notes recommended trim length 1 60 0 inch PEEK tubing ISM splitter module ccManager vent valve tee 0 007 inches Optional trim up to 30 inches 2 28 0 i...

Page 69: ...be description From To ID Notes recommended trim length 1 60 0 inches PEEK tubing ISM splitter module ccManager vent valve tee 0 007 inches Optional trim up to 30 inches 2 28 inches PEEK tubing CM A o...

Page 70: ...November 16 2016 715004521 Rev C Page 70 5 6 Core 4 configuration system diagrams and tubing connections Figure 5 26 CM 30S column configuration...

Page 71: ...4 configuration with a PDA ELSD and splitter The following table describes final tubing connections and does not list factory installed tubing connections 73 Solvent tray ELSD CM 30S PDA ccBSM SM FL c...

Page 72: ...n From To ID Notes recommended trim length 1 29 5 inch PEEKsil pre connected to the splitter Splitter module ELSD 50 m Do not trim 2 46 inch pre connected to the splitter CM 30S or PDA Splitter module...

Page 73: ...spectro meter 50 m Do not trim 2 50 inch PEEK tubing pre connected to the splitter Splitter module Left side of the ccManager tee 0 010 inches Do not trim more that 8 inches 3 59 inch PEEK tubing pre...

Page 74: ...configuration with a PDA ISM and QDa Tube Tube description From To ID Notes recommended trim length 1 60 0 inch PEEK tubing ISM splitter module ccManager vent valve tee 0 007 inches Optional trim up t...

Page 75: ...Da Tube Tube description From To ID Notes recommended trim length 1 60 0 inch PEEK tubing ISM splitter module ccManager vent valve tee 0 007 inches Optional trim up to 30 inches 2 28 inch PEEK tubing...

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Page 77: ...e ACQUITY UPC 2 Photodiode Array Detector Overview and Maintenance Guide ACQUITY UPC 2 Sample Manager Fixed Loop Operator s Overview and Maintenance Guide 6 2 Maintaining the ABPR The ccManager s ABPR...

Page 78: ...be configured to allow for a bypass To set the operation mode to enable Bypass 1 From the console system tree select the column manager 2 From the Configure menu select Operating mode 3 In the Operat...

Page 79: ...ters to provide proactive service and support for the ACQUITY UPLC system To use Connections INSIGHT you must install its service agent software on your workstation In a client server system the servi...

Page 80: ...following safety considerations when performing maintenance procedures Warning To avoid contact with biologically hazardous materials always wear appropriate personal protective equipment while handli...

Page 81: ...and source can be hot To avoid burn injuries take great care while working with these components Notice When performing maintenance inside the source enclosure ensure that the following criteria are m...

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Page 83: ...the failure of individuals to comply with any safety precaution when installing repairing or operating any of its instruments or devices The following symbols warn of risks that can arise when you op...

Page 84: ...ese precautions when working in the vicinity of such tubing when it is pressurized Wear eye protection Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expo...

Page 85: ...dures Warning To avoid personal contamination with biohazards toxic materials or corrosive materials you must understand the hazards associated with their handling Guidelines prescribing the proper us...

Page 86: ...ktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren Avvertenza qualsiasi modifica o alterazione apportata a questa unit e non espressamente autorizzat...

Page 87: ...id k nnen nichtmetallische Schl uche quellen dadurch wird der Berstdruck des Schlauches erheblich reduziert Attenzione fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione...

Page 88: ...tion assur e par le mat riel risque d tre d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwendung des Ger tes die eingebauten Sicherheitseinrichtungen unter...

Page 89: ...ez toujours les fusibles par d autres du type et de la puissance indiqu s sur le panneau proximit du couvercle de la boite fusible de l instrument Vorsicht Zum Schutz gegen Feuer die Sicherungen nur m...

Page 90: ...sicht Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen deren Typ und Nennwert im Abschnitt Sicherungen ersetzen des Kapitels Wartungsverfahren angegeben sind Attenzione per garantir...

Page 91: ...and their associated statements can appear on labels affixed to the packaging in which instruments devices and component parts are shipped Safety ground Frame or chassis terminal Fuse Functional groun...

Page 92: ...November 16 2016 715004521 Rev C Page 92 Upper limit of temperature Lower limit of temperature Temperature limitation Symbol Description...

Page 93: ...L increments Maximum operating pressure and maximum flow rate 3 25 mL min up to 41 369 kPa 413 bar 6000 psi 4 mL min to a pressure of 29 303 kPa 293 bar 4250 psi ABPR Range 10 000 to 400 000 kPa 100 t...

Page 94: ...esistivity Degree 2 refers only to normally nonconductive pollution Occasionally however expect a temporary conductivity caused by condensation Moisture protection Normal IPXO IPXO means that no Ingre...

Page 95: ...nstruction to determine chemical compatibility Table B 5 Co solvent usage Recommended Solvents up to 55 Acceptable additives up to 0 2 Solvents not recommended Methanol Formic acid Toluene Ethanol Tri...

Page 96: ...ure Specification Number of solvents co solvent pump only Liquid CO2 on the A side of the pump The CO2 high pressure inlet connects to the rear panel of the ccManager As many as four co solvents on th...

Page 97: ...a colored coded permanent label near the end of each tube pH range 2 0 to 12 0 Compressibility compensation Automatic and continuous Solvent and seal wash containers The side mounted solvent tray can...

Page 98: ...VDC Logic Low 1 9 VDC Minimum pulse width 100 msec Screw terminal connector Start gradient input Same as Stop flow input Ethernet RJ45 connector Table B 12 ccManager performance specifications Featur...

Page 99: ...i ABPR flow rate operating range Minimum 0 5 mL min Maximum flow rate 4 0 mL min Leak management Drip trays manage all leaks from the ccManager into a drain tube at the front of the module and empty t...

Page 100: ...ks and archives achiral Viridis columns Table B 14 CM 30S performance specifications Feature Specification Column capacity Eight selectable columns when bypass and waste are omitted Up to 4 6 mm inter...

Page 101: ...ty 5 at 2 0 AU propylparaben series at 257 nm 10 mm analytical flow cell Nitrogen purge Purge fitting present on optics bench and rear panel Noise wet 10 AU 254 nm 2 Hz 1 sec TC 3 6 BW res 10 mm analy...

Page 102: ...plication Sample temperature range 4 0 to 40 0 C settable in 0 1 C increments assumes an ambient temperature of 25 0 C At an ambient temperature of 21 0 C or lower the SM FL will maintain the temperat...

Page 103: ...ification Height 22 9 cm 9 inches Width 34 3 cm 13 5 inches Depth 66 cm 26 inches Weight 33 kg less than 75 pounds Table B 19 CM A Attribute Specification Height 19 9 cm 7 8 inches Width 34 3 cm 13 5...

Page 104: ...on Height 19 3 cm 7 6 inches Width 34 3 cm 13 5 inches Depth 60 7 cm 23 9 inches Weight 15 9 kg 35 0 pounds Table B 23 SM FL physical specifications Attribute Specification Height 27 1 cm 10 7 inches...

Page 105: ...two 30 cm single zone column managers instead of one Because of this it is frequently referred to as a dual CM 30S with primary and secondary ovens C 1 Physical arrangement Figure C 1 Example of the...

Page 106: ...M 30S modules running Empower software See also To configure software for a system with one column manager see the console online Help Requirement Use Empower 3 FR2 or later C 3 0 1 Setting up a prima...

Page 107: ...appears throughout the console and in the control panels C 3 0 2 Configure the operating mode for each column manager You must configure the software to accord with the fluid paths of the dual system...

Page 108: ...ow of sample and solvent to the unused column manager Example Select a column in the primary column manager then select None in the secondary primary manager C 3 0 3 1 Selecting a column before runnin...

Page 109: ...ction Do not select Bypass C 3 0 3 3 Selecting a column in a control panel or console When you select a column in a control panel the Column Selection field in the other column manager s control panel...

Page 110: ...nager is not listed first in the table the system is not configured correctly Follow the procedure Setting up a primary and secondary column manager on page 106 Result ACQ CM 2 indicating the second c...

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Summary of Contents for ACQUITY UPC2

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