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Table 3–5: Additional functions in the QSM-R control panel

Control panel
function

Description

Start up system

Brings the system to operational conditions after an
extended idle period or when switching to different
solvents.

See: 

Console online Help

Prime solvents

Displays the Prime Solvents dialog box.

Prime seal wash

Starts or stops priming the seal wash.

Wash plungers

Initiates the plunger wash sequence.

Launch Console

Launches the console software.

Reset module

Resets the QSM-R following an error condition.

Help

Displays the console online Help.

3.3.3 2489 UV-Vis control panel

The 2489 UV/Visible detector’s control panel displays absorbance units and
wavelength values. When the detector is running in dual mode, the values for both
wavelength A and B appear.

Figure 3–3: 2489 UV/Vis Detector (single mode) control panel

Lamp LED – Mirrors the lamp status LED on the front panel of the detector,
unless communications with the detector are interrupted.

Lamp ignition – When clicked, ignites or extinguishes the lamp. If the icon is
green, the lamp is ignited. If the icon is red, the lamp is extinguished.

Value of wavelength A – Displays the value of wavelength A, in nm. If the
detector is in dual wavelength mode, the value of wavelength B also appears.

June 12, 2015, 715004747 Rev. A

Page 38

Summary of Contents for ACQUITY Arc System

Page 1: ...ACQUITY Arc System Guide 715004747 Revision A Copyright Waters Corporation 2015 All rights reserved ...

Page 2: ...rporation LAC ETM is a trademark of Waters Corporation MassLynx is a registered trademark of Waters Corporation TORX is a registered trademark of Camcar Division Textron Inc Tygon is a registered trademark of Saint Gobain Performance Plastics Corporation PHILLIPS is a registered trademark of Phillips Screw Company THE SCIENCE OF WHAT S POSSIBLE is a registered trademark of Waters Corporation Custo...

Page 3: ...aters Web site Conventional mail Waters Corporation Global Support Services 34 Maple Street Milford MA 01757 USA Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical biological or radiological hazards or any combination thereof You must know the potentially hazardous effects of all substances you work with Always follow Good Laboratory Practice...

Page 4: ...ternal areas marked with the symbol first put the instrument in Standby mode Bottle placement prohibition Prohibited To avoid injury from electrical shock or fire and damage to the equipment do not place vessels containing liquid atop the workstation or ancillary equipment or otherwise expose those units to dripping or splashing liquids Prohibited Do not place vessels containing liquid such as sol...

Page 5: ... a comprehensive list of warning advisories and notices Operating the system When operating the system follow standard quality control QC procedures and the guidelines presented in this section Applicable symbols Symbol Definition Manufacturer Date of manufacture Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community...

Page 6: ...l who operate and maintain the ACQUITY Arc system Intended use of the ACQUITY Arc system Waters designed the ACQUITY Arc system to perform liquid chromatography separations in these environments Pharmaceutical development and discovery Quality assurance and quality control Chemical materials Environmental Food safety The system is not intended for use in diagnostic applications Calibrating To cali...

Page 7: ...ith Canadian ICES 001 Cet appareil numérique de la classe A est conforme à la norme NMB 001 ISM classification ISM group 1 class B This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical ISM instrument requirements Group 1 products apply to intentionally generated and or used conductively coupled radio frequency energy that is necessary for the inter...

Page 8: ...e notice v Safety advisories v Operating the system v Applicable symbols v Audience and purpose vi Intended use of the ACQUITY Arc system vi Calibrating vi Quality control vii EMC considerations vii Canada spectrum management emissions notice vii ISM classification ISM group 1 class B vii EC authorized representative vii 1 ACQUITY Arc system 12 1 1 Features of the ACQUITY Arc system 12 1 1 1 Flow ...

Page 9: ...1 Powering on the system 32 3 2 Monitoring module LEDs 33 3 2 1 Power LED 33 3 2 2 Status LEDs 33 3 3 Monitoring from control panels 35 3 3 1 SM FTN R control panel 35 3 3 2 QSM R control panel 37 3 3 3 2489 UV Vis control panel 38 3 3 4 2998 PDA control panel 39 3 3 5 2424 ELS control panel 40 3 3 6 2475 FLR control panel 41 3 3 7 2414 RI control panel 43 3 4 Opening the console 44 3 4 1 Opening ...

Page 10: ...ections 50 5 1 1 External wiring connections 51 5 1 2 Plumbing connections 52 5 1 3 Waste tubing connections 53 5 1 4 Connecting to the electricity source 54 5 1 5 Connecting signal cables 54 5 2 Ethernet connections 57 A Safety advisories 58 A 1 Warning symbols 58 A 1 1 Specific warnings 59 A 2 Notices 60 A 3 Bottles Prohibited symbol 61 A 4 Required protection 61 A 5 Warnings that apply to all W...

Page 11: ...ngth selection 74 C Specifications 80 C 1 System specifications 80 C 2 Quaternary Solvent Manager R specifications 80 C 3 Sample Manager FTN R specifications 82 C 4 30 cm Column heater and 30 cm Column heater and column heater cooler specifications 83 C 5 30 cm Column Heater Active CH 30A specifications 84 C 6 Instrument control specifications 84 C 7 Environmental specifications 85 C 8 Electrical ...

Page 12: ...ures and capabilities Pump and sample manager design enhancements that minimize dispersion and reduce cycle time Plug and play method compatibility with HPLC or UHPLC Direct programing of gradients in units of pH and ionic strength using Auto Blend Plus technology to vary composition eliminating the need to manually prepare mobile phases Automatic management of gradient start time and pre injectio...

Page 13: ...olvent s injection onto the column occurs only during auto dilution when it is used as the dilution solvent 1 1 4 Software enhancements 1 1 4 1 Quantum Synchronization Introducing a low pressure sample into the high pressure fluid stream during injection causes a pressure pulse that can affect chromatographic results The Quantum Synchronization feature reduces the affect of this pressure pulse The...

Page 14: ... the solvent manager s Automatic Prime function the system primes the lines of the optional solvent selection valve when a new line is selected You can specify the flow rate and duration of the prime for the new solvent line Example If a first injection uses line D1 and a second injection uses line D2 the solvent manager primes line D2 between the first and second injections 1 1 4 6 Flow Ramping U...

Page 15: ...llustration depicts an ACQUITY Arc system stack that includes four core modules Figure 1 1 Instrument modules Detector Column module Quaternary Solvent Manager R QSM R Sample Manager FTN R SM FTN R The ACQUITY Arc core system includes these modules Quaternary solvent manager Sample manager flow through needle June 12 2015 715004747 Rev A Page 15 ...

Page 16: ... The 30 cm Column Heater 30 cm CH and 30 cm Column Heater Cooler 30 cm CHC are the standard column heating and column heating cooling modules that manage and maintain column temperature for ACQUITY Arc systems Except for the 30 cm CHC module s cooling capability and the additional components required to support this capability the modules are identical The 30 cm CH module can control compartment t...

Page 17: ...stem using only one column compartment at a time 1 1 5 5 Detection The optical detectors compatible with your system detect and quantify concentrations of sample analyte The system accommodates these detectors 2998 PDA 2489 UV Vis 2414 RI 2475 FLR 2424 ELS Note Although the same model number is utilized each of these HPLC optical detectors have specific part numbers to be ordered with the ACQUITY ...

Page 18: ...so stores fixed column manufacturing data including unique column identification certificate of analysis QC test data When you attach the eCord fob to the receptacle on the column compartment the chip automatically records and stores system information You need take no further action Restriction The 30 cm CH and 30 cm CHC modules do not contain eCord fob functionality Although eCord fobs can be us...

Page 19: ...view and Maintenance Guide 2475 Multi Fluorescence Detector Overview and Maintenance Guide 2424 Evaporative Light Scattering Detector Overview and Maintenance Guide Auto Blend Plus Technology for Ion Exchange Size Exclusion and Reversed phase Chromatography Addendum ACQUITY QDa Detector Overview and Maintenance Guide June 12 2015 715004747 Rev A Page 19 ...

Page 20: ...s Filter buffers with a 0 45 µm filter membrane Keep concentrated stock solutions to use when preparing working solutions and refrigerate it when not using it to maximize shelf life Do not add fresh buffer to old a practice known as topping off doing so can promote microbial growth Do not block the degasser s vent line trim the tubing if necessary Do not submerge the waste or degasser vent lines i...

Page 21: ...he system Method conditions also affect carryover Too short a hold time at the final conditions of a gradient especially if the gradient is steep can fail to remove all analytes from the system It is important to completely flush the system and re equilibrate the column before proceeding to a subsequent analysis Use caution when choosing the load ahead and loop offline options Initiating these opt...

Page 22: ...5 Preventing leaks Preventing leaks during an analysis ensures adequate flow pressure in the system and the integrity of the sample Leaks can occur at any tubing connection gasket or seal but are most common at tubing connections Low pressure leaks on the intake side of the solvent manager s pump cause solvent loss and air introduction during the intake cycle Leaks at high pressure fittings downst...

Page 23: ...s during maintenance see the console online Help Whenever you loosen fittings during maintenance examine for cracks stripped threads and deformations Do not reuse stainless steel fittings more than six times 2 5 1 1 Long 1 4 28 fitting with flangeless ferrule and stainless steel lock ring installed on 1 8 inch OD tubing First use or re installed Compression screw Lock ring Ferrule End of lock ring...

Page 24: ...ew Lock ring Ferrule End of lock ring with larger inside diameter ID Tighten the fitting finger tight 2 5 1 3 5 16 24 fitting with filter and stainless steel lock ring First use or re installed Compression screw Lock ring Ferrule and filter End of lock ring with larger inside diameter ID Tighten the fitting finger tight June 12 2015 715004747 Rev A Page 24 ...

Page 25: ...ainless steel ferrule Tighten the fitting finger tight plus an additional 3 4 turn using a 1 4 inch open end wrench Tip To prevent bandspreading ensure the tubing is fully bottomed in the fitting before tightening the compression screw Re installed Long flats Compression screw 2 piece stainless steel ferrule June 12 2015 715004747 Rev A Page 25 ...

Page 26: ... 2 5 1 5 Stainless steel gold plated fitting with short flats and 2 piece stainless steel ferrule First use Short flats Compression screw 2 piece stainless steel ferrule Tighten the fitting finger tight plus an additional 3 4 turn using a 1 4 inch open end wrench Tip To prevent bandspreading ensure that the tubing is fully bottomed in the fitting before tightening the compression screw June 12 201...

Page 27: ... a 1 4 inch open end wrench Tip To prevent bandspreading ensure that the tubing is fully bottomed in the fitting before tightening the compression screw 2 5 1 6 PEEK fitting with PEEK ferrule and stainless steel lock ring First use or re installed Compression screw Lock ring Ferrule Tighten the fitting finger tight June 12 2015 715004747 Rev A Page 27 ...

Page 28: ...ferrule into the inlet of the column or in line filter 3 Finger tighten the gold compression screw into the inlet of the column or in line filter 4 Engage the column or in line filter and tighten the column or in line filter onto the gold compression screw 5 Tighten the cap nut onto the gold compression screw Important When re installing this fitting examine the captive ferrule for damage and repl...

Page 29: ...onto the dual threaded finger tight fitting To re install this fitting 1 Loosen the cap nut from the dual threaded finger tight fitting 2 Loosen and remove the dual threaded fitting with captive ferrule from the column inlet 3 Examine the captive ferrule for any damage and replace it if necessary Important For detailed instructions about replacing the ferrule see Replacing the ferrule on the colum...

Page 30: ...ell volume is the system volume between the point where the gradient is formed and the column inlet The dwell volume varies from system to system but is typically between 0 1 and 2 0 mL You can measure dwell volume using the midpoint of a 0 100 gradient To do so run a gradient between two identical solvents A and B spiking the B solvent spiked with a marker Perform the measurement after configurin...

Page 31: ...th technology offers plug and play method compatibility with HPLC and UHPLC methods In addition still or circulated air column modules are available to emulate the heating behavior of an original system 2 9 Matching sample diluents When you use the sample manager s auto dilution option the purge solvent serves as the sample diluent Ensure that your sample solution is soluble and miscible in your c...

Page 32: ... system s workstation Result The following start up tests run CPU board memory RAM and ROM external communication system Ethernet and clock Important If the system includes a 30 cm column heater cooler power on the device by toggling the power switch on the rear right hand side of the unit before powering on the sample manager 2 Power on the solvent manager and sample manager by pressing the power...

Page 33: ... glows green when power is applied to the device and unlit when power is not applied Note To provide adequate ventilation the sample manager s fans run continuously even when the power switch is in the off position These fans switch off only when you disconnect the power cable from the ac wall outlet or rear panel 3 2 2 Status LEDs 3 2 2 1 Run LED Run status is indicated by an LED on the sample ma...

Page 34: ...color Indication Unlit The solvent manager is idle Steady green The solvent manager is operating normally flow is moving through the system as programmed Flashing green The solvent manager is initializing Flashing red An error stopped the solvent manager Refer to the console log for information about the error Alternative Firmware upload is in progress Steady red A failure is preventing operation ...

Page 35: ...s You can monitor the sample manager solvent manager column module and detector from control panels which you access via the chromatography data system When Empower software controls the system the control panels appear at the bottom of the Run Samples window When MassLynx software controls the system the control panels appear on the Additional Status tab of the Inlet Editor window You can update ...

Page 36: ...point for the column compartment Selected column Displays the currently selected column Current column compartment temperature Displays the current temperature in the column compartment Status Displays the status of the current operation You can access these additional functions by right clicking anywhere in the SM FTN R control panel Table 3 4 Additional functions in the SM FTN R control panel Co...

Page 37: ...n clicked immediately stops all flow from the solvent manager Solvent composition Displays the percentage of solvent to be drawn from solvent lines A through D Composition values range from 0 0 to 100 0 Valve position Displays the solvent manager path currently in use Flow rate Displays the flow rate of solvent through all lines of the solvent manager System pressure Displays system pressure in kP...

Page 38: ...online Help 3 3 3 2489 UV Vis control panel The 2489 UV Visible detector s control panel displays absorbance units and wavelength values When the detector is running in dual mode the values for both wavelength A and B appear Figure 3 3 2489 UV Vis Detector single mode control panel Lamp LED Mirrors the lamp status LED on the front panel of the detector unless communications with the detector are i...

Page 39: ...nches the console software Reset module Resets the detector after an error condition Help Displays console online Help 3 3 4 2998 PDA control panel The 2998 Photodiode Array detector s control panel displays the detector status Figure 3 4 2998 PDA detector control panel Lamp LED Mirrors the lamp status LED on the front panel of the detector unless communications with the detector are interrupted L...

Page 40: ...s control panel displays light scattering units photomultiplier tube gain factor gas pressure nebulizer temperature and drift tube temperature Lamp LED Mirrors the lamp status LED on the front panel of the detector unless communications with the detector are lost Current column heater temperature Displays the current column heater temperature Column heater set point Displays the column heater set ...

Page 41: ...set point Displays the set point for the drift tube temperature Current drift tube temperature Displays the current drift tube temperature You can access these additional functions by right clicking anywhere in the detector control panel Table 3 8 Additional functions in the 2424 ELS detector control panel Control panel function Description Autozero Resets the detector offsets Launch Console Launc...

Page 42: ...ichannel spectrum scanning or 3D Em λ Displays the emission wavelength Ex λ Displays the excitation wavelength Photomultiplier tube gain factor Displays the current photomultiplier tube gain factor Status Displays the status of the current operation You can access these additional functions by right clicking anywhere in the detector control panel Table 3 9 Additional functions in the 2475 FLR dete...

Page 43: ...rature set point Displays the set point for the flow cell temperature Peak polarity Displays the polarity of the output signal If the polarity is negative the chromatogram is inverted Status Displays the status of the current operation You can access these additional functions by right clicking anywhere in the detector control panel Table 3 10 Additional functions in the 2414 RI detector control p...

Page 44: ...ntrol panel for any module and select Launch Console from the menu that appears 3 4 2 Opening the console from MassLynx software To open the console 1 From the MassLynx window click Inlet Method 2 Click the Additional Status tab Result A control panel for each device in the system appears 3 In the sample manager control panel click Display console Alternative Right click the control panel for any ...

Page 45: ...nutes Ensure that sufficient quantities of solvent are available for priming Tip In the console you can select the Startup System feature to prime all solvents and to specify the solvent composition flow rate column and sample temperatures and needle characterization For details see the console online Help June 12 2015 715004747 Rev A Page 45 ...

Page 46: ...ature of the symptom Serial number of the system module and its firmware version if applicable Flow rate Operating pressure Solvent s Detector settings sensitivity and wavelength Type and serial number of column s Sample type and diluent Chromatography data software version and serial number System workstation model and operating system version Note For an explanation about how to report shipping ...

Page 47: ...riodic maintenance schedules that ensure optimal performance of the system modules and therefore performance of the system The maintenance frequencies shown apply to modules that receive moderate use When using the system throughout the day and on nights and weekends or when using aggressive solvents such as buffers perform these maintenance tasks more frequently See also For complete procedures r...

Page 48: ...ormation about the support needs of a system directly to Waters If you encounter a performance problem when using the console software manually submit a Connections INSIGHT request to Waters Customer Support Alternatively use Remote Desktop a real time collaboration option that controls the two way connection with the system by enabling the Connections INSIGHT iAssist service level These sources a...

Page 49: ...an help you determine when to schedule routine maintenance for specific components You can specify usage thresholds and maintenance warnings that alert you when a component reaches a specified threshold Thus you can minimize unexpected failures and unscheduled downtime during important work For information explaining how to specify maintenance warnings consult the Waters console Help June 12 2015 ...

Page 50: ...unpacks and installs the system components Warning To avoid spinal or muscular injury do not attempt to lift the system components without assistance If you must transport a system component or remove it from service request recommended cleaning flushing and packaging procedures from Waters Technical Service 5 1 External wiring and plumbing connections June 12 2015 715004747 Rev A Page 50 ...

Page 51: ...5 1 1 External wiring connections Figure 5 1 System rear panel wiring connections Column module 30 cm CHC shown Detector SM FTN R QSM R June 12 2015 715004747 Rev A Page 51 ...

Page 52: ...5 1 2 Plumbing connections Figure 5 2 System plumbing connections Detector SM FTN R QSM R Column module 30 cm CHC shown June 12 2015 715004747 Rev A Page 52 ...

Page 53: ...5 1 3 Waste tubing connections Figure 5 3 System waste tubing connections Solvent tray Detector SM FTN R QSM R Column module 30 cm CHC shown June 12 2015 715004747 Rev A Page 53 ...

Page 54: ...ce it if necessary Power off and unplug each module before performing any maintenance operation on it Connect each module to a common ground Recommendation Use a line conditioner and uninterruptible power supply UPS for optimum long term input voltage stability To connect to the electricity source 1 Connect the female end of the power cord to the receptacle on the rear panel of the module 2 Connec...

Page 55: ...ns cables 1 Using the flat blade screwdriver attach the positive and negative leads of the signal cable to the connector Figure 5 4 Positive and negative lead connections 1 2 3 June 12 2015 715004747 Rev A Page 55 ...

Page 56: ...Inserting connector into connector port 1 2 Connector port Connector 3 Fit the grounding cable s fork terminal on the rear panel grounding stud and secure the terminal using the locking nut Note Use the 9 32 inch nut driver to tighten the locking nut until the terminal does not move June 12 2015 715004747 Rev A Page 56 ...

Page 57: ...t accommodates the PC workstation and as many as six system modules Connect the shielded Ethernet cables from each module to the electronic connections on the rear panel of the sample manager The sample manager is connected internally to the Ethernet switch Alternative Some column modules connect to the sample manager using a D sub cable assembly June 12 2015 715004747 Rev A Page 57 ...

Page 58: ...y or property damage resulting from the failure of individuals to comply with any safety precaution when installing repairing or operating any of its instruments or devices The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device or component of an instrument or device When one of these symbols appear in a manual s narrative sections or procedur...

Page 59: ...ments and devices fitted with nonmetallic tubing Warning To avoid injury from bursting nonmetallic tubing heed these precautions when working in the vicinity of such tubing when it is pressurized Wear eye protection Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to compounds with which it is chemically incompatible tetrahydrof...

Page 60: ...ments and devices that can process biohazards corrosive materials or toxic materials Warning To avoid personal contamination with biohazards toxic materials or corrosive materials you must understand the hazards associated with their handling Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council s publication Prudent Prac...

Page 61: ...ply to all Waters instruments and devices When operating this device follow standard quality control procedures and the equipment guidelines in this section Warning Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Avertissement Toute modification sur cette unité n ayant pas été expressément...

Page 62: ...o not use nonmetallic tubing with tetrahydrofuran THF or concentrated nitric or sulfuric acids Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic tubing to swell which greatly reduces the rupture pressure of the tubing Avertissement Manipulez les tubes en polymère sous pression avec precaution Portez systématiquement des lunettes de protection lorsque vous vous trouvez à pro...

Page 63: ...rcostante Non utilizzare tubi eccessivamente logorati o piegati Non utilizzare tubi non metallici con tetraidrofurano THF o acido solforico o nitrico concentrati Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precaución cuando se trabaje con tub...

Page 64: ... Nonmetallic 튜브를 테트라히드로푸란 Tetrahydrofuran THF 또는 농축 질 산 또는 황산과 함께 사용하지 마십시오 염화 메틸렌 Methylene chloride 및 디메틸술폭시드 Dimethyl sulfoxide 는 비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오 警告 圧力のかかったポリマーチューブを扱うときは 注意してください 加圧されたポリマーチューブの付近では 必ず保護メガネを着用してください 近くにある火を消してください 著しく変形した または折れ曲がったチューブは使用しないでください 非金属チューブには テトラヒドロフラン THF や高濃度の硝酸または硫酸などを流さないでくださ い 塩化メチレンやジメチルスルホキシドは 非金属チューブの膨張を引き起こす場合があり その場 合 チューブは極め...

Page 65: ...ncia el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante las medidas de protección del equipo podrían ser insuficientes 警告 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用 那麼該設備所 提供的保護將被消弱 警告 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用 那么该设备所 提供的保护将被削弱 경고 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다 警告 ユーザーは 製造元により指定されていない方法で機器を使用すると 機器が...

Page 66: ... 경고 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한 타 입 및 정격의 제품으로 퓨즈를 교체하십시오 警告 火災予防のために ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプお よび定格のヒューズをご使用ください Finding fuse types and ratings when that information does not appear on the instrument or device Warning To protect against fire replace fuses with those of the type and rating indicated in the Replacing fuses section of the Maintenance Procedures chapter Avertis...

Page 67: ...정격의 제품으로 퓨즈를 교체하십시오 警告 火災予防のために ヒューズ交換ではメンテナンス項目の ヒューズの交換 に記載されているタ イプおよび定格のヒューズをご使用ください A 7 Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument s front or rear panels Symbol Description Electrical power on Electrical power off Standby Direct current Alternating current Alternating current 3 phase Safety ground F...

Page 68: ...s can appear on labels affixed to the packaging in which instruments devices and component parts are shipped Symbol Description Keep upright Keep dry Fragile Use no hooks Upper limit of temperature Lower limit of temperature Temperature limitation June 12 2015 715004747 Rev A Page 68 ...

Page 69: ...001307 available on the Waters Web site Visit www waters com B 1 1 Clean solvents Clean solvents ensure reproducible results and permit you to operate with minimal instrument maintenance Dirty solvents can cause baseline noise and drift and they can clog solvent reservoir filters inlet filters and capillary lines B 1 2 Solvent quality Use MS grade solvents for the best possible results the minimum...

Page 70: ...ive system volumes of HPLC grade distilled or deionized water When using a buffer choose good quality reagents filtering them through a 0 2 μm membrane filter For shutdowns of more than a day s duration flush the pump with a 20 methanol water solution to prevent microbial growth Notice Some buffers can be incompatible with mass spectrometers Consult the documentation that accompanies your instrume...

Page 71: ...ane 29 at 20 C 101 32 12 Ethyl acetate 73 at 20 C 77 11 4 Ethyl alcohol 43 9 at 20 C 78 32 15 Ethyl ether 442 at 20 C 34 55 45 Ethylene dichloride 83 35 at 20 C 83 48 13 Heptane 35 5 at 20 C 98 43 4 Hexane 124 at 20 C 68 7 22 Iso octane 41 at 20 C 99 24 12 Isobutyl alcohol 8 8 at 20 C 107 7 28 Isopropyl alcohol 32 4 at 20 C 82 26 12 Isopropyl myristate 1 at 20 C 182 6 164 Methanol 97 at 20 C 64 7 ...

Page 72: ...er require an intermediate solvent like n propanol Temperature affects solvent miscibility If you are running a high temperature application consider the effect of the higher temperature on solvent solubility Buffers dissolved in water can precipitate when mixed with organic solvents When you switch from a strong buffer to an organic solvent thoroughly flush the system with distilled water before ...

Page 73: ...e difference between two M numbers is 15 or less the two liquids are miscible in all proportions at 15 C A difference of 16 indicates a critical solution temperature from 25 to 75 C with 50 C as the optimal temperature If the difference is 17 or greater the liquids are immiscible or their critical solution temperature is above 75 C Some solvents prove immiscible with solvents at both ends of the l...

Page 74: ...osity Generally viscosity is not a consideration when you use a single solvent or under low pressure In gradient chromatography however the viscosity changes that occur as the solvents are mixed in varying proportion can effect pressure changes during the run If you do not know the extent to which pressure changes affect the analysis monitor the pressure during the run B 2 4 Wavelength selection T...

Page 75: ...ce using Beer s law because absorbance is proportional to concentration Note λ cutoff is the wavelength at which the absorbance of the solvent equals 1 AU Table B 4 Wavelength cutoffs for different mobile phases Mobile phase UV cutoff nm Acetic acid 1 230 Ammonium acetate 10 mM 205 Ammonium bicarbonate 10 mM 190 Polyoxyethylene 35 lauryl ether BRIJ 35 0 1 190 3 3 cholamidopropyl dimethylammonio 1 ...

Page 76: ...de 1 M 207 Sodium citrate 10 mM 225 Sodium dodecyl sulfate 190 Sodium formate 10 mM 200 Triethylamine 1 235 Trifluoracetic acid 0 1 190 TRIS HCl 20 mM pH 7 0 202 TRIS HCl 20 mM pH 8 0 212 Triton X 100 0 1 240 Waters PIC Reagent A 1 vial liter 200 Waters PIC Reagent B 6 1 vial liter 225 Waters PIC Reagent B 6 low UV 1 vial liter 190 Waters PIC Reagent D 4 1 vial liter 190 June 12 2015 715004747 Rev...

Page 77: ...es are based on a 10 mm path length Table B 5 Mobile phase absorbance measured against air or water Absorbance AU at specified wavelength nm 200 205 210 215 220 230 240 250 260 280 Solvents Acetonitrile 0 05 0 03 0 02 0 01 0 01 0 01 Methanol not degassed 2 06 1 00 0 53 0 37 0 24 0 11 0 05 0 02 0 01 Methanol degassed 1 91 0 76 0 35 0 21 0 15 0 06 0 02 0 01 Isopropanol 1 80 0 68 0 34 0 24 0 19 0 08 ...

Page 78: ...d HEPES 10 mM pH 7 6 2 45 2 50 2 37 2 08 1 50 0 29 0 03 0 01 Morpholinoethanesulfonic acid MES 10 mM pH 6 0 2 42 2 38 1 89 0 90 0 45 0 06 0 01 Potassium phosphate monobasic KH2PO4 10 mM 0 03 0 01 Potassium phosphate dibasic K2HPO4 10 mM 0 53 0 16 0 05 0 01 0 01 Sodium acetate 10 mM 1 85 0 96 0 52 0 30 0 15 0 03 0 01 Sodium chloride 1 M 2 00 1 67 0 40 0 10 0 01 Sodium citrate 10 mM 2 48 2 84 2 31 2...

Page 79: ... L 0 01 0 01 PIC D4 1 vial L 0 03 0 03 0 03 0 03 0 02 0 02 0 02 0 02 0 02 0 01 BRI J 35 1 0 06 0 03 0 02 0 02 0 02 0 01 0 01 3 3 cholamidopropyl dimethylammonio 1 propanesulfonate CHAPS 0 1 2 40 2 32 1 48 0 80 0 40 0 08 0 04 0 02 0 02 0 01 Sodiumdodecyl sulfate SDS 0 1 0 02 0 01 0 01 4 octylphenol polyethoxylate Triton X 100 0 1 2 48 2 50 2 43 2 42 2 37 2 37 0 50 0 25 0 67 1 42 Polyoxyethylene sor...

Page 80: ...lay of diagnostic data via the console Cycle time 30 seconds injection to injection C 2 Quaternary Solvent Manager R specifications Table C 2 QSM R specifications Attribute Specification Solvent capacity Total capacity of nine solvents with optional integrated solvent selection valve blends as many as four solvents in any combination standard Number of fluid paths Three Path 1 Path 2 and waste wit...

Page 81: ...oise 0 5 mAU average peak to peak with appropriately sized filter or mixer mobile phase containing 0 1 TFA in water acetonitrile 1 5 mL min CORTECS C18 2 7 µm 4 6 x 50 mm 30 C UV 214 nm Composition accuracy 0 5 absolute full scale from 5 to 95 0 5 to 5 0 mL min methanol methanol with 5 0 mg mL caffeine step gradient UV 273 nm Composition precision 0 15 RSD or 0 04 min SD whichever is greater based...

Page 82: ...6 well plate 384 well plate 48 position 0 65 mL micro centrifuge tube plate 24 position 1 50 mL micro centrifuge plate Sample compartment temperature 4 0 40 0 C settable in 0 1 C increments optional Temperature accuracy 0 5 C at the sensor Temperature stability 1 0 C at the sensor Injection needle wash Integral active and programmable Minimum sample required 3 µL residual using Waters Maximum Reco...

Page 83: ...m CH 20 0 or 5 0 C above ambient to 65 0 C Column compartment temperature accuracy 0 5 C at the sensor Test qualification Temperature measured by a NIST traceable probe located next to the measurement sensor must fall within this specification Column compartment temperature stability 0 3 C at the sensor Over temperature stability Note Thermal switch shuts off heater at 80 C 2 8 C temperature measu...

Page 84: ...partment heater at 130 C 5 C temperature measured at the thermal switch APH Assembly Thermal fuse shuts off APH heater cartridge indefinitely at 140 C 5 C temperature measured at thermal fuse Leak control Compartment drip tray with leak sensor installed under compartment Single exit drain manages leaks to waste Solvent conditioning Active pre heating Column tracking eCord technology tracks column ...

Page 85: ...erature range 4 0 to 40 0 C Operating humidity range 20 to 80 non condensing C 8 Electrical specifications Table C 8 ACQUITY Arc electrical specifications Attribute Specification Power requirements 100 to 240 VAC Line frequency 50 to 60 Hz Power consumption QSM R 200 VAC SM FTN R 400 VAC 30 cm CHC 240 VAC 30 cm CH 50 W CH30 A 50 W C 9 Physical specifications This table provides the physical specif...

Page 86: ...ACQUITY Arc system physical specifications Attribute Specification Width 57 4 cm 22 6 in Height 57 1 cm 22 5 in Depth 62 8 cm 24 7 in Weight 59 1 kg 130 0 lbs June 12 2015 715004747 Rev A Page 86 ...

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