3-16
Maintaining the Autosampler
3.
Disconnect the plunger from the syringe drive by sliding it straight back
and out of the holding clip.
4.
Slide the plunger out of the syringe barrel.
5.
Using pliers, remove the plunger tip.
6.
Dampen the new tip with an appropriate solvent (e.g., isopropanol).
7.
Mount the new tip on the plunger.
8.
Insert the plunger into the syringe barrel.
9.
Fill the new syringe with degassed wash solvent.
Recommendations:
•
Use isopropanol as the wash solvent.
•
Remove air bubbles from the syringe.
10. Connect the plunger of the filled syringe to the syringe drive by pushing
it straight onto the clip.
Requirement:
Keep the top threaded end below the syringe valve.
11. Raise the syringe, and screw it firmly into the connector.
12. In the Replace Sample Syringe window, click Reset.
Result:
The syringe moves to the home position and dispenses its
contents to waste.
13. Via the Control menu of the 2707 Console, perform a standard wash
routine.
Result:
All tubing connected to the syringe valve refills and flushes.
14. Purge all air bubbles from the syringe and buffer tubing by repeatedly
washing the needle or removing and repriming the syringe with
degassed, nonaqueous organic solvent.
Replacing the syringe dispenser valve
The syringe valve is a 4-port selection valve. One port is unused. These are
port assignments:
•
Waste – Drain for the syringe dispenser.
•
Wash – Port for aspirating wash liquid from the wash bottle
