June 11, 2015, 715004752 Rev. A
Page 44
2.7.1 Connecting columns in HPLC systems
The plumbing connections to the detector are located in the front, left-hand side of
the flow cell assembly.
To connect the inlet and outlet tubing in an HPLC system:
1. Attach the stainless steel compression fitting and ferrule (supplied in the startup
kit).
2. Connect the inlet tubing to the column outlet.
3. Ensure that the tubing is seated firmly in the column outlet, and then tighten
the compression screw.
4. Connect the Tygon
tubing to the flow cell outlet tubing, and route the tubing to a
waste container.
2.7.2 Connecting columns in ACQUITY Arc systems
The plumbing connections to the detector are located in the front, left-hand side of
the flow cell assembly.
Note:
Tubing assemblies are supplied in the appropriate system fluidic kit.
To connect the inlet and outlet tubing in an ACQUITY Arc system:
1. Connect the inlet tubing to the column outlet, and then to the flow cell inlet.
2. Connect the outlet tubing to the flow cell outlet, and route it to a waste
container.
Notice:
To avoid damaging the flow cell or tubing, do not connect any
tubing or device that can cause the backpressure to exceed the pressure
rating of those components. The pressure rating of a standard analytical
flow cell is 6895 kPa (69 bar, 1000 psi).
Warning:
To prevent injury, always observe Good Laboratory Practices when
you handle solvents, change tubing, or operate the system. Know the physical
and chemical properties of the solvents you use. See the Material Safety Data
Sheets for the solvents in use.
Warning:
To prevent injury, always observe Good Laboratory Practices when
you handle solvents, change tubing, or operate the system. Know the physical
and chemical properties of the solvents you use. See the Material Safety Data
Sheets for the solvents in use.
Notice:
To avoid equipment damage caused by spilled solvent, do not
place fluid containers directly atop an instrument or device without a
solvent tray to contain spills.
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