Waters 2489, Operator'S Manual, Page: 98 / 234

3-30 Preparing the Detector
5. Set the pump flow rate in your HPLC system to 1.0 mL/min.
6. Inject 1
µ
L acetone.
When the test is successful, your chart recorder or data system displays a
peak.
Wavelength calibration
You can calibrate the detector manually from the keypad. You can use the
manual calibration key to recalibrate the detector at any time during
operation, or if there are any calibration errors during startup. You do not
have to reboot the detector after a successful wavelength calibration.
To calibrate the detector manually:
1. Press Calibrate (Shift 3) from the detector keypad.
A message appears asking whether you removed the cuvette and flushed
the flow cell with a transparent solvent (Waters recommends methanol
or water).
Cuvette wavelength calibration message
2. Press Enter to continue the calibration cycle, or press Cancel to return to
the absorbance screen without calibrating the detector.
After you press Enter, the detector cycles through the calibration
procedure and briefly displays a series of initialization messages similar
to those you saw at startup (see
“Initializing the detector” on page 3-2 ).
If calibration is successful, the detector beeps three times and displays
the maximum error in nanometers of the furthest calibration shift from
the last calibration.