Waters 2475, Operator'S Manual, Page: 36 / 228

1-22 Theory of Operation
Wavelength selection
In fluorescence, if the excitation monochromator is set below the UV cutoff of a
mobile-phase component, the solvent absorbs some of the available excitation
light intensity, which in turn reduces the fluorescence emission response for
the sample. For a complete list of UV cutoff ranges for common solvents and
common mixed mobile phases, refer to Appendix C .
Warning:
Using incompatible solvents can cause severe damage to
the instrument and injury to the operator.