Waters 2475, Operator'S Manual, Page: 210 / 228

C-10 Solvent Considerations
UV cutoffs for common solvents
The following table shows the UV cutoff (the wavelength at which the
absorbance of the solvent is equal to 1 AU for some common chromatographic
solvents). Operating at an excitation wavelength near or below the cutoff
increases baseline noise due to the solvent’s ability to absorb excitation light
energy.
UV cutoff wavelengths for common chromatographic solvents
Solvent UV Cutoff (nm) Solvent UV Cutoff (nm)
1-Nitropropane 380 Ethylene glycol 210
2-Butoxyethanol 220 Iso-octane 215
Acetone 330 Isopropanol 205
Acetonitrile 190 Isopropyl
chloride
225
Amyl alcohol 210 Isopropyl ether 220
Amyl chloride 225 Methanol 205
Benzene 280 Methyl acetate 260
Carbon disulfide 380 Methyl ethyl
ketone
330
Carbon
tetrachloride
265 Methyl isobutyl
ketone
334
Chloroform 245 Methylene
chloride
233
Cyclohexane 200 n -Pentane 190
Cyclopentane 200 n -Propanol 210
Diethyl amine 275 n -Propyl chloride 225
Dioxane 215 Nitromethane 380
Ethanol 210 Petroleum ether 210
Ethyl acetate 256 Pyridine 330
Ethyl ether 220 Tetrahydrofuran 230
Ethyl sulfide 290 Toluene 285
Ethylene
dichloride
230 Xylene 290