Waters 2475, Operator'S Manual, Page: 110 / 228

3-38 Using the Detector
can also use the Energy setting on channel B while making the primary
fluorescence measurement specified by a EUFS on channel A. For
example, while operating in single-channel mode, you can set an EUFS
of 500 on the second channel, providing a different scaling factor on the
channel B 1-V output.
The detector engages the second-order filter for all excitation wavelengths
greater than 400 nm.
You can configure the detector to show the measurement results in either
emission units or energy units (see page 3-19 ).
Operating the detector in multichannel mode
You can operate the detector with expanded chart-out selections in
multichannel (
λλ)
mode, which offers the following functions:
• Emission (A and B)
• Sample energy (A and B)
• Reference energy (A and B)
• MaxPlot
• Difference (A-B) or (B-A)
For details about these functions, see page 3-19 and the table titled “Primary
and secondary function (method) parameters” on page 3-21 .
Changing from single to multichannel mode
To change from single to multichannel mode:
1. From the home screen in single-channel mode ( λ ), select λ/λλ (Shift,
Auto Zero).
This key toggles between single and multichannel mode, and the
detector displays a message indicating it is setting up multichannel
operation.
2. Specify the excitation wavelength to monitor in the x
λ
field, and then
press Enter.
3. Specify the emission wavelength to monitor in the e λ field, and then
press Enter.