Waters 2475, Operator'S Manual, Page: 106 / 228

3-34 Using the Detector
saturated, highly distorted measurements when you apply them to the
2475 detector. You must therefore reduce the 2475 detector’s gain
setting as much as tenfold when using a method developed for the 474
detector. A gain setting of 1 suffices in most cases because of the
2475 detector’s favorable signal-to-noise ratio.
• Sample Energy or Emission Units – Empower or Millennium
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software
can accept either sample energy or emission units. You can edit the data
field Channel Description when creating an Empower or Millennium
instrument method to record the units used, selecting the units from the
Analog Out field on the second of the four home screens.
• Run Time – The run time is not synchronized with that of Empower or
Millennium
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software. However, the run time (and therefore
programmed timed events) is synchronized with the recorded time axis
of the chromatogram in Empower and Millennium
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software.
Remote control operation via Ethernet connection using 2475
instrument control software
This mode of operation uses the Ethernet connection and appears in the
Empower software Configuration window as a 2475 detector. The remote
control also icon appears on the detector’s home screen (see the figure
“Fluorescence home screen” on page 3-3 ) with an “E” at it’s center (see the
table titled “2475 detector home and message screen icons” on page 3-5 ). For
this mode of operation, you must disable the Emulate 474 option from the
Empower Configuration window.
Verifying the detector
Perform tests to verify wavelength accuracy (see page 3-35 ) and optimize
emission units (see page 3-36 ). Doing so ensures no components in the flow
cell interfere with the erbium lines at 379 and 522 nm. Completing these
procedures successfully ensures that the detector’s optics and electronics
function properly.
Tip:
Before you pump solvent or mobile phase through the system, flush the
lines with filtered, degassed, and sparged HPLC-grade water. Then pump the
mobile phase, provided you encounter no miscibility problems, at 1 mL/min for
15 minutes, minimum.