Waters 2414, Overview And Maintenance Manual

Page: 113 / 130

June 11, 2015, 715004754 Rev. A
Page 113
Refer to the following table to troubleshoot problems with qualitation or quantitation.
Table 7–6: Incorrect results troubleshooting
Symptom Possible cause Corrective action
Decreased peak height Leak in injector Troubleshoot injector.
Degraded, contaminated,
or improperly prepared
sample
Use fresh sample.
Column contaminated Clean or replace column.
Loss of column efficiency Clean or replace column.
Change in mobile phase
composition
Correct mobile phase pH
or ionic composition.
Incorrect flow rate Change flow rate.
Dirty flow cell
Electronic noise Clean the fluid path (see
“Decontaminating the fluid
path” on page 93 ).
Increased noise Use the shortest cable
lengths possible.
Ensure that cables are
shielded.
Dirty flow cell Clean the fluid path (see
“Decontaminating the fluid
path” on page 93 ).
Air bubble in flow path Replace column with union
and purge flow path at 10
mL/min.
Mobile phase not degassed Degas mobile phase.
Contaminated mobile
phase
Use fresh mobile phase.