Waters 1500-Series, Operator'S Manual

Page: 182 / 188

C-14 Solvent Considerations
Refractive indices of common solvents
The following table lists the refractive indices for some common
chromatographic solvents. Use this table to verify that the solvent you intend
to use for your analysis has a refractive index (RI) significantly different from
the RIs of the sample components.
EDTA, disodium, 1 mM 190 TRIS HCl, 20 mM, pH 7.0,
pH 8.0
202, 212
HEPES, 10 mM, pH 7.6 225
Triton-X
™
100, 0.1%
240
Hydrochloric acid, 0.1% 190 Waters PIC Reagent A,
1 vial/liter
200
MES, 10 mM, pH 6.0 215 Waters PIC Reagent B-6,
1 vial/liter
225
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
190
190
Waters PIC Reagent B-6,
low UV, 1 vial/liter
190
Sodium acetate, 10 mM 205 Waters PIC Reagent D-4,
1 vial/liter
190
Refractive indices for common chromatographic solvents
Solvent RI Solvent RI
Fluoroalkanes 1.25 Tetrahydrofuran (THF) 1.408
Hexafluoroisopropanol
(HFIP)
1.2752 Amyl alcohol 1.410
Methanol 1.329 Diisobutylene 1.411
Water 1.33 n -Decane 1.412
Acetonitrile 1.344 Amyl chloride 1.413
Ethyl ether 1.353 Dioxane 1.422
n -Pentane 1.358 Ethyl bromide 1.424
Acetone 1.359 Methylene chloride 1.424
Wavelength cutoffs for different mobile phases (Continued)
Mobile phase
UV
cutoff
(nm)
Mobile phase
UV
cutoff
(nm)