Walz MINI-PAM-II, Manual, Page: 40 / 130

Chapter 5 Saturation Pulse Analysis
34
Table 3: Fluorescence Ratio Parameters.
Source Equation
Sample
State
Range
[Theory]
[Experiment]
Maximum photochemical quantum
yield of PS II (Kitajima and Butler,
1975)
M
M
M
V
F
F F
F
F
0
−
=
Dark
[0, 1]
[0, ~0.84]
Effective photochemical quantum
yield of PS II (Genty et al. , 1989)
M
M
F
F F
II Y
′
−
′
= ) (
Light
[0, 1]
[0,~ 0.84]
Quantum yield of light-induced
(ΔpH- and zeaxanthin-dependent)
non-photochemical fluorescence
quenching (Genty et al . 1996)*
M M
F
F
F
F
NPQ Y
−
′
= ) (
Dark and
Light
[0, 1]
[0, ~ 0.9]
Quantum yield of non-regulated
heat dissipation and fluorescence
emission: this type of energy loss
does not involve the action of a
trans-thylakoid ΔpH and zeaxan-
thin (Genty et al . 1996)*
M
F
F
NO Y
= ) (
Dark and
Light
[0, 1]
[0, ~ 0.9]
Stern-Volmer type non-
photochemical fluorescence
quenching (and Björkman,
1990)
1 −
′
=
M
M
F
F
NPQ
Dark and
Light
[0, ∞]
[0, ~4]
Coefficient of photochemical fluo-
rescence quenching (Schreiber et
al. 1986 as formulated by van
Kooten and Snel, 1990)
0
F F
F F
q
M
M
P
′
−
′
−
′
=
Light. If F
0
’
calculated,
Dark and
Light
[0, 1]
[0, 1]
Coefficient of photochemical fluo-
rescence quenching assuming in-
terconnected PS II antennae
(Kramer et al. 2004)
F
F
q q
P L
0
′
⋅ =
As q
P’.
[0, 1]
[0, 1]
Coefficient of non-photochemical
fluorescence quenching
(Schreiber et al. 1986 as formulat-
ed by van Kooten and Snel, 1990)
0
0
1
F F
F F
q
M
M
N
−
′
−
′
− =
Dark and
Light
[0, 1]
[0, ~0.95]
* Kramer et al. (2004) have derived more complex equations for Y(NO) and Y(NPQ).
Klughammer and Schreiber (2008) have demonstrated that the equations by Kramer et
al. (2004) can be transformed into the simple equations of (Genty et al . 1996) which are
used by the MINI-PAM-II and WinControl-3.