Fluid Operation
General Fluid Operation
128
MultiMode SPM Instruction Manual
Rev. B
1. Install the protective O-ring into the fluid cell. Insert the O-ring into the recessed groove in
the underside of the fluid cell. The O-ring slides up into the recessed groove.
2. Install the sample or sample support in the fluid cell.
3. Install the fluid cell in the AFM head, and tighten the clamp to hold the fluid cell in place,
making certain that the O-ring positions properly between the sample and fluid cell. Position
the fluid cell in the head to ensure that the O-ring creates a seal on the sample around the
periphery of the fluid cell and does not overlap any edges of the sample. If necessary, use the
XY translation knobs on the head to center the O-ring.
Figure 8.2c
Fill the Fluid Cell with Liquid
4. Verify the head is leveled side-to-side and that the head is tilted slightly forward, so the tip is
level when it contacts the surface.
Note:
If you are using a vertical-engage scanner, leveling problems are eliminated.
5. Using the
Tip Down
switch on the MultiMode base (and the coarse adjustment screws if not
using a vertical engage scanner), lower the tip toward the sample until the tip is within
~30µm of the surface. This will bring the fluid cell close enough to the surface to ensure that
the O-ring will create a seal against the sample.
6. Fill the fluid cell with liquid.
Fluid cell installed in
MultiMode head.
Syringe
Buffer Solution
