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Troubleshooting
NOTE: If, after performing these tests, you are still unable to isolate the cause of
the problem, contact your Varian Customer Support Representative.
What To Do If You Experience a Loss of High Mass Peaks
The loss of high mass peaks maybe due to:
•
RF ramp needs Adjustment
•
Too many low mass ions (i.e., air or water leak)
•
Improper Ionization storage levels (i.e., settings are too low)
•
High Trap temperatures may cause loss of high mass Cal Gas peaks
Before you begin troubleshooting, however, be sure that Saturn has baked out
for at least 2 hours, and that the manifold temperature is at or below 50
°
C. If
you have done this, and the problem persists, continue as follows.
1. Check for an air leak in Auto Tune Section.
2. Check RF ramp Adjustment
3. Reduce trap temperature to 150
°
C.
4. Enter Method editor, check method contains EI AGC ionization mode, and
Default values for other parameters.
NOTE: If, after performing these tests, you are still unable to isolate the cause of
the problem, contact your Varian Customer Support Representative.
What To Do If Part of the Spectrum is Missing
If you do not observe high- or low-mass ions in System Control but the ions in the
mid-range of the spectrum appear normal, you should investigate the following
possibilities:
•
An RF adjustment may be required, particularly if you have just changed
the ion trap temperature.
•
The ionization RF level may be incompatible with the scan range.
•
The trap temperature may be too high to allow you to observe all of the
cal gas ions. Reduce trap oven temperature to 150
°
C, and wait 2 hours
for thermal equilibration.
Before you begin troubleshooting, however, be sure that Saturn has baked out
for at least 2 hours. If you have done this, and the problem persists, continue as
follows.
Check the RF Adjustment
Check whether an RF ramp adjustment is needed, proceeding as follows:
1. Enter
System
Control.
2. Select Adjustments, and click on Adjust RF Ramp
