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Varian, Inc. 
2700 Mitchell Drive 
Walnut Creek, CA 94598-1675/usa 

 

©Varian, Inc. 2003-2006 

Printed in U.S.A. 

03-914981-00:Rev. 3 

 

 

1200 LC/MS APCI Option 

 

 

 

 

 

Atmospheric Pressure Chemical 

Ionization (APCI)

 

Operator’s Manual

 

NOTICE: Varian, Inc. was acquired by Agilent 

Technologies in May 2010. This document is provided 

as a courtesy but is no longer kept current and thus 

will contain historical references to Varian. For more 

information, go to 

www.agilent.com/chem

. 

Summary of Contents for 1200 LC/MS

Page 1: ...0 LC MS APCI Option Atmospheric Pressure Chemical Ionization APCI Operator s Manual NOTICE Varian Inc was acquired by Agilent Technologies in May 2010 This document is provided as a courtesy but is no...

Page 2: ...ically reproduced distributed or printed in its entirety provided this copyright and statement are attached Any modification or any other reproduction distribution or use of this document or portions...

Page 3: ...we live up to a standard of quality that you can count on today and into the future Our Quality System has received ISO 9001 certification number FM21797 The quality systems that earned us ISO 9001 r...

Page 4: ...e instrument or accessory Repair or replacement under warranty does not extend the original warranty period Repair or replacement under warranty claims shall be made in Varian s sole discretion either...

Page 5: ...Qualit tsstandard bieten auf den Sie heute und in Zukunft rechnen k n nen Unser Qualit tssystem hat die ISO 9001 Zertifikatnummer FM21797 erhalten Die Qualit tssysteme der ISO 9001 Registrierung haben...

Page 6: ...die urspr ngliche Garantiezeit Reparatur oder Austausch unter Garantieanspr chen soll in Varian s ausschlie lichem Ermessen entweder durch einen Serviceingenieur beim Kunden oder durch Erm chtigung de...

Page 7: ...garantir et de maintenir un niveau de qualit Lesdites proc dures ont re u l homologation ISO 9001 num ro FM21797 Les syst mes de qualit qui ont re u l homologation ISO 9001 pr sentent des avantages di...

Page 8: ...es d un recours est laiss l enti re discr tion de Varian soit par l envoi d un technicien de maintenance sur le site du client soit en autorisant le client retourner l accessoire ou l instrument d fec...

Page 9: ...quale il cliente pu contare oggi come nel futuro Il nostro Sistema di Qualit ha ottenuto la certificazione ISO 9001 col numero FM21797 I sistemi di qualit per i quali abbiamo ottenuto l omologazione I...

Page 10: ...razioni o le sostituzioni in garanzia verranno effettuate ad esclusiva discrezione della Varian inviando sul posto un tecnico o autorizzando la resa dello strumento o dell accessorio difettoso alla Va...

Page 11: ...ificaci n ISO 9001 representan beneficios directos para nuestros clientes haremos llegar nuestros aparatos m s r pidamente que nunca Podemos cumplir con pedidos urgentes a n m s deprisa Atenderemos su...

Page 12: ...prolongan el per odo de garant a original Las reparaciones o sustituciones en per odo de garant a se efectuar n a criterio exclusivo de Varian enviando un representante de servicio posventa a la inst...

Page 13: ...oderate injury and or equipment damage and how to avoid these situations Alerts you to potentially hazardous situations that could result in serious injury and how to avoid these situations Warning Sy...

Page 14: ...has been used for the analysis of hazardous biological radioactive or toxic samples prior to any instrument service being performed or when an instrument is being returned to the Service Center for re...

Page 15: ...graphy The operator should be familiar with the physico chemical properties of the components of the mobile phase Keep solvents from direct contact with the polyurethane supply tubing as certain solve...

Page 16: ...nical support and training can be provided by qualified personnel on both a contractual or as needed basis Varian Inc Analytical Instruments Sales Offices For Sales or Service assistance and to order...

Page 17: ...uf die Vermeidung dieser Situationen hin Warnungssymbol Warnungsbeschreibung WARNUNG ELEKTRISCHER SCHLAG Gef hrliche Spannungen bestehen innerhalb des Instruments Trennen Sie das Ger t vom Netz bevor...

Page 18: ...t f r Analysen gef hrlicher biologischer radioaktiver oder toxischer Proben benutzt worden ist Elektrische Gefahren L sen Sie das Instrument von allen Stromquellen bevor Sie Schutzpaneele entfernen da...

Page 19: ...durchf hren ffnen Sie niemals eine unter Druck stehende L sungsmittelleitung oder ein Ventil Halten Sie zuerst die Pumpe an und lassen Sie den Druck auf Null abfallen Benutzen Sie splittersichere Res...

Page 20: ...zung und Training bieten wir Ihnen durch qualifizierte Chemiker sowohl auf einer Kontraktbasis als auch nach Ihren Erfordernissen an Varian Analytical Instruments Verkaufsb ros F r Verkaufs oder Servi...

Page 21: ...mment rem dier cette situation Symboles d avertissement Description ATTENTION RISQUE D ELECTROCUTION Exposition des tensions dangereuses D brancher le mat riel du secteur avant de d visser les panneau...

Page 22: ...giques dangereux radioactifs ou toxiques avant que n en soit effectu la maintenance Risques de chocs lectriques D connecter le mat riel de toute source d alimentation avant d en d monter les panneaux...

Page 23: ...p rations de maintenance de routine Ne jamais d connecter un conduit de solvant ou une vanne sous pression Arr ter pr alablement la pompe et laisser la pression descendre z ro Utiliser des r servoirs...

Page 24: ...el sp cifique support technique et service de formation assur s par des chimistes qualifi s sur base contractuelle ou en fonction des besoins sp cifiques Points de vente des instruments analytiques Va...

Page 25: ...ZIONE Descrizione del Pericolo ATTENZIONE Pericolo di folgorazioni Nello strumento sono presenti tensioni pericolose Scollegare il cavo di alimentazione prima di togliere il pannello fissato con le vi...

Page 26: ...zzato per l analisi di campioni biologicamente pericolosi radioattivi o tossici Pericoli Elettrici Prima di togliere i pannelli di protezione scollegare lo strumento da tutte le alimentazioni elettric...

Page 27: ...routinaria del sistema Non smontare mai una linea del solvente od una valvola quando il sistema sotto pressione Fermare prima la pompa ed aspettare che la pressione scenda a zero Usare dei contenitor...

Page 28: ...a piu il costo delle parti A richiesta si possono avere corsi per operatori sia sotto forma di contratto che a tariffe da concordare Uffici Vendite della Divisione Strumenti Analitici della Varian Per...

Page 29: ...grosas que pueden causar serios da os y c mo evitar esas situaciones S mbolo Descripci n ATENCI N PELIGRO DE DESCARGA EL CTRICA El instrumento utiliza voltages peligrosos Desconecte el interruptor gen...

Page 30: ...stras peligrosas de origen biol gico radioactivo t xico antes de comenzar a realizar cualquier an lisis Peligros El ctricos Desconecte el instrumento de todos las conexiones el ctricas a la red antes...

Page 31: ...e rutina Nunca abra una l nea una v lvula bajo presi n Apague la bomba antes y deje que la presi n baje a cero Utilice dep sitos irrompibles que sean capaces de operar a 50 60 psi Mantenga cerrada la...

Page 32: ...a y materiales El mantenimiento y el entrenamiento se realiza por ingenieros cualificados bajo Contrato petici n Oficinas de Instrumentaci n Anal tica Varian Para cualquier consulta sobre Instrumentac...

Page 33: ...9 APCI Performance Evaluation 10 Operating Conditions for Testing the APCI 10 APCI Sensitivity Test 10 APCI General Maintenance 11 Cleaning 11 Cleaning the Spray Shield 11 Cleaning the Vacuum Capilla...

Page 34: ...03 914981 00 3 2...

Page 35: ...mplementary but significantly different in terms of setup and optimization When the appropriate criteria for APCI have been met chemical ionization offers advantages of increased sensitivity enhanced...

Page 36: ...t Attachment CH3COO M M CH3COO Also Cl HCOO Electron Capture The APCI Source consists of two distinct parts the atmospheric pressure chamber and the API capillary assembly Vaporization ionization and...

Page 37: ...Applications APCI is ideally suited to non polar and medium polarity molecules Analytes such as polynuclear aromatic hydrocarbons PAHs polychlorinated biphenyls PCBs fatty acids phthalates and triglyc...

Page 38: ...e gas lines to the nebulizer assembly Nebulizing gas top fitting Auxiliary gas bottom fitting APCI Aux Gas Yellow Needle Gas Green Nebulizing Gas N2 Connection to Needle Gas Green Nebulizing Gas ESI A...

Page 39: ...nector calibrate the needle current readback 3 Select Inst setup others from the Tools menu 4 Click Zero the APCI Needle Current and click Execute 5 After the routine is done plug the corona high volt...

Page 40: ...pray Ionization ESI chamber Refer to the Tuning section of the 1200L manual APCI Mobile Phase Selection Most common HPLC mobile phases are ideal for LC MS Normal phase solvents such as dichloromethane...

Page 41: ...iary gases as well as the temperature and flow of the drying gas Gas flows that are too low may cause an unstable baseline while gas flows that are too high may reduce the size of the observed peaks T...

Page 42: ...ophenol solution which is 2 pg L 10 pg injected APCI Sensitivity Test Positive APCI Set the SIM scan mode to mass 609 3 A signal to noise of approximately 20 1 RMS or greater should be attained for 10...

Page 43: ...lace it only if performance degrades Cleaning the Spray Shield 1 Remove the spray shield by gently rotating it off the mounting posts 2 Wipe it with methanol or if needed scrubbing with an aluminum ox...

Page 44: ...e careful of the sharp point Insert a new or cleaned corona needle into the mounting hole and press firmly until it is fully seated The alignment should not change during normal needle cleaning or rep...

Page 45: ...ng Installation Carefully feed the new needle assembly 03 937544 03 into the hole in the nebulizer assembly being careful not to bend the new needle Slide a new PEEK fingertight ferrule and nut 28 211...

Page 46: ...he bulkhead fittings on the front of the instrument 4 Disconnect liquid connection to injector or column not the fitting on top of the nebulizer 5 Disconnect the large round 14 pin connector from the...

Page 47: ...he spray chamber 2 Place the white ceramic insulating washer 03 937323 01 into the cutout on the vaporizer covering the top of the heater Gently press on the washer to seat it in the cutout 3 Take the...

Page 48: ...ch the spray chamber 10 Replace the nebulizer assembly lowering it straight down using care to first pass the nebulizing tube into the quartz tube and then to engage the alignment pins the gas lines s...

Page 49: ...uartz tube it may be because the mobile phase contains a high percentage of water flowing at a high flow rate Increase the vaporizer temperature or reduce the flow rate until the drops are no longer v...

Page 50: ......

Page 51: ...Assembly 0 004 ID 03 937337 01 Spray Shield 27 402529 00 Spray Chamber O ring 27 402690 00 O ring Spray Chamber Window 03 937323 01 Ceramic Insulating Washer white 03 920276 00 Aluminum Oxide 600 Gri...

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