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TSE Calorimetry PhenoMaster Hardware Operating Instructions Download Page 29

CaloSys Modules

   29

 

11. 

Calorimetry Measurement 

11.1. Overview Devices and Connections 

 Individual CaloSys module devices… 

…are described in Section 11.2 and following sections. Detailed information, particularly about safety 
measures, is given in the description for the individual instrument. If you are at all uncertain, 
particularly about safety-relevant measures, please contact 

TSE Service

.

 

[email protected]

 

www.TSE-Systems.com

 

 

Summary of Contents for Calorimetry PhenoMaster

Page 1: ...www TSE Systems com PhenoMaster Hardware Operating Instructions Calorimetry Sophisticated Life Science Research Instrumentation Info TSE Systems com ...

Page 2: ...and subject to due penalty at law This applies in particular to their reproduction processing translation microfilming as well as to their storage and processing in electronic systems This regulation does not apply to reproductions for internal use Further examples of these operating instructions are available and can be ordered from us a charge will be made for these extra copies PM15_HW_CaloSys_...

Page 3: ...ys Module to be operated with manual Calibration 30 11 1 1 2 CaloSys Module to be operated with automatic or manual Calibration 32 11 1 1 3 CaloSys Module including multiple Calorimetry Control Units 33 11 1 2 Pull Principle Respirometry Underpressure Principle 36 11 1 2 1 CaloSys Module to be operated with manual Calibration 36 11 1 2 2 CaloSys Module to be operated with automatic or manual Calib...

Page 4: ... about Reference Air 55 11 5 Connecting PC 55 11 6 Air Supply Pump 55 11 6 1 General 57 11 6 2 Installation 57 11 6 3 Connections 57 11 6 4 Operation 58 11 7 Vacuum Pump 59 11 7 1 Installation 60 11 7 2 Connections 61 11 7 3 Operation 62 11 8 Protection Air Filter 62 11 8 1 Connections Protection Air Filter 62 11 9 Air Drying Unit 64 11 9 1 1 1x Air Drying Unit 65 11 9 1 2 4x Air Drying Unit 66 11...

Page 5: ...on Control Unit Analog read out 8 place system 36 Fig 16 Schematic diagram of CaloSys module Pull principle respirometry w Calibration Control Unit 8 place system 38 Fig 17 Schematic diagram of CaloSys module Pull principle respirometry w multiple High Speed Sensor Units Digital read out 32 place system 41 Fig 18 Calorimetry Master Control Unit 44 Fig 19 Calorimetry Master Control Unit Rear view 4...

Page 6: ...6 PhenoMaster Hardware Operating Instructions info TSE Systems com www TSE Systems com ...

Page 7: ...enoMaster Phenotyping Research Platform of TSE Systems Some devices and features may not be available on your system For your individual system configuration please refer to your order confirmation If you have questions or need assistance please contact TSE Service See Hardware Operating Instructions Core Modules Section Introduction info TSE Systems com www TSE Systems com ...

Page 8: ...re that the device is properly ventilated Min distance o Air Drying Unit Wall 150 200mm 5 91 7 87inch o Air Drying Unit Other devices 150 200mm 5 91 7 87inch NOTICE Reduced lifetime If Calorimetry Process Control Units and Air Pumps are powered ON while there is a leakage in the sample line this may reduce lifetime of devices After you have discarded condensed water carefully reconnect collection ...

Page 9: ...rating Principle See Hardware Operating Instructions Core Modules Section Operating Principle 4 PC Requirements See Hardware Operating Instructions Core Modules Section PC Requirements info TSE Systems com www TSE Systems com ...

Page 10: ...ce driver Installation Path OS Default Installation Path Windows7 32bit C Program Files TSE PhenoMaster Drivers Bin_x86 Windows7 64bit C Program Files x86 TSE PhenoMaster Drivers Bin_x64 Windows10 32bit C Program Files TSE PhenoMaster Drivers Bin_x86 Windows10 64bit C Program Files x86 TSE PhenoMaster Drivers Bin_x64 Fig 1 Default installation path Drivers destination location Windows7 Select Wind...

Page 11: ...ation dialog Check Device Manager dialog The list item with the attention symbol should have disappeared and under Universal Serial Bus controllers a new entry USB Bulk driver should have been created info TSE Systems com www TSE Systems com ...

Page 12: ...ating Instructions 5 2 WireOne Driver Follow default installation routine Double click install_1_wire_drivers msi Press Next Check I accept the terms in the License Agreement and press Next info TSE Systems com www TSE Systems com ...

Page 13: ...CaloSys Modules 13 Confirm default installation path by pressing Next info TSE Systems com www TSE Systems com ...

Page 14: ...14 PhenoMaster Hardware Operating Instructions Press Install Press Install info TSE Systems com www TSE Systems com ...

Page 15: ...CaloSys Modules 15 Press Finish info TSE Systems com www TSE Systems com ...

Page 16: ...ting Devices General 7 Cage Rack See Hardware Operating Instructions Core Modules Section Cage Rack 8 System Software See Hardware Operating Instructions Core Modules Section System Software 9 Performing a Measurement See Hardware Operating Instructions Core Modules Section Performing a Measurement info TSE Systems com www TSE Systems com ...

Page 17: ...enance and Cleaning Cage Cage Lid 10 1 1 1 Calorimetry Cage Lid Prerequisites In order to clean and decontaminate calorimetry cage lids it is necessary to dismantle them completely WARNING Eye Injuries When the quick action coupling plug is loosened it may pops out and injure your eyes Hold the quick action coupling plug tightly when loosening it and do NOT bend down over it info TSE Systems com w...

Page 18: ...18 PhenoMaster Hardware Operating Instructions 23 1 19 15 5 10 11 21 14 18 20 13 12 22 26 6 25 3 24 19 8 7 9 17 4 2 4 16 7 6 11 info TSE Systems com www TSE Systems com ...

Page 19: ... w cross recess Makrolon clear transparent 12 19 Countersunk screw w cross recess PC M3x6 4 Makrolon clear transparent 4 20 Countersunk screw w cross recess PC M2x4 Makrolon clear transparent 4 21 Induction disk w sinking Sm2Co17 4 22 Resilient pressure piece 1 4031 steel 4 23 Component assembly digital temperature sensor Electronics 1 24 Countersunk screw M2x8 cross recess 1 4031 steel 20 25 Lens...

Page 20: ...mperature 65 C 149 F With increasing number of sterilization procedures a slight embrittlement associated with fissuring may occur Applicable 2 Peracetic acid applicable for Makrolon shaped parts Polycarbonate glued Not specified Depends on 1 glue and 2 gluing procedure Not specified Depends on 1 glue and 2 gluing procedure Not specified Depends on 1 glue and 2 gluing procedure Not specified Depen...

Page 21: ...s that must be protected against dust particles Check the air filters of the air supply pumps every month and replace them when dirty but LATEST every three months Failure to do so will void the warranty Fig 3 Air pump Top view Remove filter cover screw Fig 4 Filter cover removed Remove filter cover info TSE Systems com www TSE Systems com ...

Page 22: ...black connection tubing and remove it from the overpressure valve Decontamination Air Supply Pump In order to decontaminate the air supply pump the air filter and rubber seal see Section 10 2 1 as well as connection tubing to the overpressure valve should first be removed The filter should be decontaminated separately After the filter the rubber seal and connection tubing have been removed and the...

Page 23: ...ve clothing should be taken when filter exchange is carried out Please contact your safety manager Dispose the contaminated filter according to the national safety and environmental regulations Replace the decontaminated filter in the air supply pump before using it Overpressure valve decontamination The overpressure valve should first be removed from the pump It can be decontaminated with H2O2 va...

Page 24: ... felt when the deactivated position is reached If the diaphragm is damaged repeated triggering of diaphragm protective switch pump must be serviced by trained service personnel Thermal protection activated due to high temperature AP 60 AP 80 After cooling pump will switch back on automatically AP 30 AP 40 Check pump by trained service personnel Diaphragm damaged Service pump by trained service per...

Page 25: ... principle see Section 11 1 2 Ensure that filters are inserted in the correct flow direction i e reversed as compared to push principle There is a flow direction arrow on the filter label Note that additional disk filters see Section 10 5 are included downstream of Air OUT of animal cages NOTICE Instrument damage If protection air filters are not replaced at regular intervals contaminants could en...

Page 26: ...2 The disk filters are located downstream of Air OUT of animal cages Note that an additional air protection filter may be included upstream of Air IN of vacuum pump see Section 10 4 NOTICE Instrument damage If disk filters are not replaced at regular intervals contaminants could enter the Control Units and affect their functions or cause damage Prior to an experiment check flow rates The filters m...

Page 27: ...nt solution can also be used for moistening Detergent residues should then be wiped off After cleaning the Air Drying Units can be decontaminated with H2O2 vapor before it is brought into the test laboratory The glass collection vessels can be autoclaved New silicone tubing can be decontaminated with hot air up to 180 C 356 F Used silicone tubing should be disposed of according to the national saf...

Page 28: ...ll the battery compartment from the main housing Replace the battery standard AA battery and push the battery compartment back on the main housing By pushing the battery compartment into place you automatically connect the battery to the electronics It will take a few seconds for the meter to start up You have to press the screen for about 3 seconds in order to switch the unit on Replacing the bat...

Page 29: ...evices are described in Section 11 2 and following sections Detailed information particularly about safety measures is given in the description for the individual instrument If you are at all uncertain particularly about safety relevant measures please contact TSE Service info TSE Systems com www TSE Systems com ...

Page 30: ... be operated with manual Calibration Fig 12 Schematic diagram of CaloSys module Push principle respirometry w o Calibration Unit Analog read out 8 place system Black lines Cable connections Blue lines Tube connections Lower green frame Device used in combination with high speed sensors only info TSE Systems com www TSE Systems com ...

Page 31: ... Switch Unit 1 8 Tube connections for animal cage 1 8 Ref IN Tube connection for reference air Sample OUT Tube connection to Air Drying Unit X04 Cable connection to Calorimetry Master Control Unit Air Drying Unit Sample IN Tube connection to sample switch pump Sample OUT Tube connection to Calorimetry Master Control Unit via separating bottle Air Pump Air outlet Tube connection to Calorimetry Mast...

Page 32: ... manual Calibration Fig 13 Schematic diagram of CaloSys module Push principle respirometry w Calibration Control Unit 8 place system Black lines Cable connections Blue lines Tube connections Lower green frame Device used in combination with high speed sensors only info TSE Systems com www TSE Systems com ...

Page 33: ...t Connection to Calibration Control Unit Sample IN Tube connection to Air Drying Unit via separating bottle Sample OUT Tube connection to Calorimetry Master Control Unit Gas Inlets1 4 Tube connections to calibration gas cylinders via pressure regulator automatic calibration PC X01 PCI 9111 37 pin cable connection to Calorimetry Master Control Unit X02 PCI 9111 37 pin cable connection to Calorimetr...

Page 34: ...ection between Calorimetry Master Control Unit and Calorimetry Slave Control Unit if available Fig 14 Schematic diagram of CaloSys module Push principle respirometry w multiple Calorimetry Control Units Digital read out 8 place system Black lines Cable connections Blue lines Tube connections Treadmill Process Control Unit CaloTreadmill cable connections not shown info TSE Systems com www TSE Syste...

Page 35: ...l cage CaloTreadmill 3 4 OUT2 Tube connection to Air Drying Unit2 for animal cage CaloTreadmill 3 4 9 10 Tube connections for animal cage CaloTreadmill 5 6 OUT3 Tube connection to Air Drying Unit3 for animal cage CaloTreadmill 5 6 1 2 Tube connections for animal cage CaloTreadmill 7 8 OUT4 Tube connection to Air Drying Unit4 for animal cage CaloTreadmill 7 8 X04 25 pin connection to Calorimetry Ma...

Page 36: ... operated with manual Calibration Fig 15 Schematic diagram of CaloSys module Pull principle respirometry w o Calibration Control Unit Analog read out 8 place system Black lines Cable connections Blue lines Tube connections Lower green frame Device used in combination with high speed sensors only info TSE Systems com www TSE Systems com ...

Page 37: ...resh air supply Sample Tube connection to Sample Switch Control Unit Sample Switch Control Unit 1 8 Tube connections for animal cage 1 8 Ref IN Tube connection for reference air Sample OUT Tube connection to Air Drying Unit X04 25 pin connection to Calorimetry Master Control Unit Air Drying Unit Sample IN Tube connection to sample switch pump Sample OUT Tube connection to Calorimetry Master Contro...

Page 38: ... manual Calibration Fig 16 Schematic diagram of CaloSys module Pull principle respirometry w Calibration Control Unit 8 place system Black lines Cable connections Blue lines Tube connections Lower green frame Device used in combination with high speed sensors only info TSE Systems com www TSE Systems com ...

Page 39: ...n for reference air Sample OUT Tube connection to Air Drying Unit X04 25 pin connection to Calorimetry Master Control Unit Air Drying Unit Sample IN Tube connection to sample switch pump Sample OUT Tube connection to Calibration Control Unit via separating bottle Calibration Control Unit X0 ext Connection to Calibration Control Unit Sample IN Tube connection to Air Drying Unit via separating bottl...

Page 40: ...e example a 32 place system includes 2 x 1 Calorimetry Master Control Units 2 x 1 Calorimetry Slave Control Units 2 x 1 Sample Switch Control Units 2 x 4 Air Drying Units 1 x Calibration Master Control Unit 2 x 2 Calibration Slave Control Unit 2 x 4 High Speed Sensor Units 1 x PC info TSE Systems com www TSE Systems com ...

Page 41: ...ions Fig 17 Schematic diagram of CaloSys module Pull principle respirometry w multiple High Speed Sensor Units Digital read out 32 place system Black lines Cable connections Blue lines Tubing connections info TSE Systems com www TSE Systems com ...

Page 42: ...System_2 Ref Air2 4 Tube connection to reference cage2 System_1 reference cage4 System_2 X06 Connection to Calorimetry Master Control Unit Temperature 1 8 Cable connection temperature sensors Vacuum Pump1 2 Vac inlet Tube connection to Calorimetry Control Unit via pressure control valve Animal Cage Air OUT Tube connection to Calorimetry Control Unit used air outlet Air IN Inlet fresh air supply Sa...

Page 43: ...Pumps are powered ON without operating the system this may reduce lifetime of devices Always run system software when devices are powered ON Power OFF devices after use The Control Unit provides the connection between animal cages PC animal cages and the air pump respectively Depending on the CaloSys module configuration one or more Control Units may be necessary one of which is always the Calorim...

Page 44: ...tion 11 1 1 1 For instructions about connections of a Calorimetry Control Unit of CaloSys modules working with push principle to be operated with automatic or manual calibration see Section 11 1 1 2 For instructions about connections of a Calorimetry Control Unit of CaloSys modules working with pull principle see Section 11 1 2 Fig 19 Calorimetry Master Control Unit Rear view 1 CO2 zero point rese...

Page 45: ...es the connections of a Calorimetry Master Control Unit of a CaloSys module working with push principle to be operated with manual calibration compare Section 11 1 1 1 For instructions about connections of a Calorimetry Master Control Unit of CaloSys modules working with push principle to be operated with automatic or manual calibration compare Section 11 1 1 2 11 1 1 3 For instructions about conn...

Page 46: ...w System configuration This section describes the connections between a Calorimetry Master Control Unit and a High Speed Sensor Unit of a CaloSys module to be operated with manual calibration see Sections 11 1 1 1 push principle 11 1 2 1 pull principle For instructions about the connections between High Speed Sensor Unit and PC of a CaloSys module to be operated with automatic or manual calibratio...

Page 47: ...is connected to the X05 port on the Calorimetry Master Control Unit if available If not there can be an O2 CO2ext connection between Calorimetry Master Control Unit and Calorimetry Slave Control Unit Reference cage One of the Flow OUT ports is reserved for the reference cage Mass Flow Controllers in Calorimetry Control Unit 11 2 2 Mass Flow Controllers MFC are used to control the mass flow of the ...

Page 48: ...ns and preferences Before you put the unit into operation verify that the unit is suitable for your application gas range pressure and temperature You find these details on the supplied calibration sheet If any of these details deviate from your application please contact TSE Service Fig 22 Flowmeter 1 Air inlet 2 Air outlet 3 Power Display of battery charge level w 3 segments 4 Display of actual ...

Page 49: ...rate and a new calibration procedure should be carried out Lock the setting you have made with the locking lever A standard CaloSys module with rat cages type III requires a flow of approx 1 5 2 8l min according to the weight the strain medical treatment etc of the animal one with mouse cages requires a flow of approx 0 4l min Also take the information about calibration given above into account If...

Page 50: ...works according to the paramagnetic principle For further instructions see Hardware Operating Instructions High Speed Sensor Unit 11 2 4 2 CO2 Sensor Working Principle The CO2 sensor consists of an infrared light source a gas cell a narrow optical filter and a detector The sensor uses the Non Dispersive InfraRed NDIR gas sensing method CO2 molecules just like other molecules vibrate and rotate at ...

Page 51: ...odule with automatic calibration Connections Calibration Master Process Control Unit 11 3 1 System configuration This section describes connections of a Calibration Master Control Unit of a CaloSys module without Calibration Slave Control Units see Sections 11 1 1 2 push principle 11 1 2 2 pull principle For instructions about connections of a Calorimetry Master Control Unit of CaloSys modules wit...

Page 52: ...ing Unit Sample OUT connects to the Sample IN of the Calorimetry Control Unit For automatic calibration the calibration gas cylinders should be connected via pressure regulator to the Gas Inlets 1 3 of the rear panel see Fig 26 4 Observe instructions given below what MAX calibration gas pressures can be applied when calibration gas cylinders are connected to the Gas Inlet of the Calibration Master...

Page 53: ...ector without using a pressure reducing valve set to 5bar 72psi above atmospheric pressure Gas Inlet1 and 2bar 29psi above atmospheric pressure Gas Inlet2 3 respectively This warning is given on the instrument attached onto the rear panel NOTICE Instrument damage Too high operating pressure will damage the internal sensor of the High Speed Sensor Unit and the air pump membrane Never connect a gas ...

Page 54: ...armful gases from the plastic foam block Always remove the transportation safety device before use NOTICE Instrument damage Operating with the safety device can cause uncontrolled warming of sample air Always remove the transportation safety device before use Connections Sample Switch Unit 11 4 1 Fig 28 Ports Connectors of Sample Switch Unit for 16 animal cages System configuration This section de...

Page 55: ...ions 11 1 1 1 push principle 11 1 2 1 pull principle For instructions about connections of the PC of CaloSys modules to be operated with manual or automatic calibration see Section 11 1 1 2 push principle 11 1 2 2 pull principle For instructions about connections of the PC of CaloSys modules retrofitted for automatic calibration see Section 11 3 1 Three cable connections are used to connect the PC...

Page 56: ...agnets connected in parallel The armature is attracted and repelled by the magnetic field resulting in a linear oscillating motion with high efficiency and without frictional loss A diaphragm is fixed on both ends of the armature which with a chamber block and valves forms an air pump In case of a diaphragm rupture an integrated protective switch turns off the pump In case of fault the thermal pro...

Page 57: ...izontal installation position Ensure the pump stands on a firm base With a suitable slip free surface no further fixing is necessary Do NOT place the pump anywhere where there is a risk of penetration by water oil or any other liquid Never set up and operate the pump in a potentially explosive environment The following substances must NOT be allowed to enter the air intake dangerous gas mixtures e...

Page 58: ...nnection to T piece The flexible black tubing of the air pump Air OUT is connected via a T piece to the pressure control valve Downstream the T piece is connected to the Flow IN inlet see Fig 12 of the Calorimetry Control Unit via an air protection filter NOTICE Instrument damage The opening for the pressure release of the pressure control valve must not be allowed to become dirty as this could li...

Page 59: ...without operating the system this may reduce lifetime of devices Always run system software when devices are powered ON Power OFF devices after use Vacuum pumps allow to generate and maintain the set airflow within the CaloSys module The pump is suitable exclusively for air and NOT for pumping liquids or gases Max permissible humidity 90 Under no circumstances you should grease or oil the pump The...

Page 60: ...le is damaged then repairs and service work must be carried out by suitably qualified personnel ONLY Installation 11 7 1 DANGER Hazardous voltage Improper selection of installation place can cause an electrical shock or start fire The pump position must be easily accessible well ventilated and even Do NOT place the pump anywhere where there is a risk of penetration by water oil or any other liquid...

Page 61: ...k must be carried out by suitably qualified personnel ONLY Fig 33 Pressure control valve 1 Tube connection to T piece The tubing of the vacuum pump Air IN is connected via a T piece to the pressure control valve Upstream the T piece is connected to the Flow OUT inlet see Fig 15 of the Calorimetry Control Unit via an air protection filter Ensure that tubing isn t rough kinked tightly bent and that ...

Page 62: ...rection i e reversed as compared to push principle There is a flow direction arrow on the filter label Observe safety instructions regarding filter replacement intervals compare Section 11 9 1 CAUTION Damage to Control Units and Air pumps Note that additional disk filters are included downstream of Air OUT of animal cages see Section 10 5 To protect the mass flow controllers in Calorimetry Control...

Page 63: ...w down the screw nuts to both filter ends and pass a tubing end through each of the screw nuts Push the tubing ends over the two filter connections and then screw down the screw nuts tightly so that the tubing cannot become loosened accidentally Filter installation Ensure that the filter is inserted in the correct flow direction There is a flow direction arrow on the filter label Fig 35 Flow direc...

Page 64: ...e device must be properly ventilated Air Drying Unit location Position the Air Drying Unit so that it is at a lower level than the Unit with the O2 and CO2 sensors thus any condensate will always run off into the separating bottle Before the sample flow enters the Calorimetry Control Unit and the O2 and CO2 analyzer the sample flow should have moisture removed from it so that no condensed water ca...

Page 65: ...e must be discarded when they are half full of condensate at the LATEST in order to keep the amount of moisture entering the tubing connections as low as possible NOTICE Reduced lifetime If Calorimetry Process Control Units and Air Pumps are powered ON while there is a leakage in the sample line this may reduce lifetime of devices After you have discarded condensed water carefully reconnect collec...

Page 66: ...e amount of moisture entering the tubing connections as low as possible To drain off a clamp attached to the tubing must be opened Do NOT remove collection vessels The condensed water of the separating bottle must be discarded when it is half full of condensate at the LATEST in order to keep the amount of moisture entering the tubing connections as low as possible NOTICE Reduced lifetime If Calori...

Page 67: ...tions Air Drying Unit 11 9 2 Tubing length The tubing connections from and to the Air Drying Unit should be kept as short as possible and must not be kinked NOTICE Measurement errors The sensors in the Calorimetry Control Unit are sensitive measuring instruments that must be protected against humidity There should be no condensed water in the tubings via the O2 and CO2 sensors Check the tubing reg...

Page 68: ... power consumption W Voltage Industrial PC 560 1 560 50Hz 230V PC monitor 30 1 30 Printer 75 1 75 Air pump 150 1 150 CaloSys Control Unit 200 1 200 CaloSys Control Unit for Valves 100 1 100 Air Drying Unit 100 4 400 CaloSys Calibration Unit 100 2 200 Treadmill Control Unit 250 1 250 Treadmill 75 8 600 Total 2565 Under normal operation conditions Power On peak is higher and can be calculated from m...

Page 69: ...CaloSys Modules 69 info TSE Systems com www TSE Systems com ...

Page 70: ...stems com Sophisticated Life Science Research Instrumentation Specifications subject to change without notice Copyright 2017 TSE Systems International Group All rights reserved https www tse systems com support technical support form ...

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