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Trace2O AquaSafe WSL50, Instruction Manual, Page: 3 / 52

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Section 1: Introduction 

The AquaSafe® WSL50 is a fully portable field laboratory for the detection of microbiological and 
physico-chemical water quality parameters.   

The lab is housed in a waterproof wheeled carry case with telescopic handle and comprises the 
following:  

•

 

Integrated dual chamber digital incubator complete with all accessories and consumables for 
carrying out 300 tests for Faecal and Total Coliforms. 

•

 

Block Tester complete with consumables for 300 tests of Free & Total Chlorine and pH. 

•

 

Turbidity Tube for turbidity testing in the range 5 to 500NTU. 

 

 

 

 

 

 

Summary of Contents for AquaSafe WSL50

Page 1: ...AquaSafe WSL50 Water Safety Laboratory Instruction Manual...

Page 2: ...ts Section 1 Introduction Section 2 Kit Contents Section 3 Double Incubator Instructions Section 4 Membrane Filtration Instructions Section 5 Turbidity Tube Instructions Section 6 Block Tester Instruc...

Page 3: ...d in a waterproof wheeled carry case with telescopic handle and comprises the following Integrated dual chamber digital incubator complete with all accessories and consumables for carrying out 300 tes...

Page 4: ...Section 2 Kit Contents 1 2 3 4 5 5 Under Accessories Box 6 7 8 a b c d e f g h i j k k l b b a b...

Page 5: ...Tablets c 1 5ml Sterile syringe d 1 30ml Dropper Bottle for Methanol e 1 Membrane Filtration Unit including waste beaker sample beaker measuring funnel sintered glass disk 1 silicone gasket f 1 Steril...

Page 6: ...Tablets 100 Pack 3 HP2 DPD No 1 Rapid Tablets 100 Pack 3 HP3 DPD No 3 Rapid Tablets 100 Pack Separate Box Qty Description 4 Sterilised Water for Membrane Lauryl Sulfate Broth Preparation 2 Sterile Me...

Page 7: ...Section 3 AquaSafe Micro biological Incubator Instruction Manual...

Page 8: ...sequence Right Chamber 17 3 3 3 Start screen Figure 1 1 18 3 3 4 37 C Program Fig 1 2 19 3 3 5 44 C Program Fig1 3 20 3 3 6 User defined Program Fig 1 4 21 3 3 7 Resetting the timer Fig 1 5 22 3 3 8...

Page 9: ...x 3 5mm aluminium petri dishes which are suitable for 47mm membrane filter pads but the incubator can also be used with 55mm pre prepared plastic petri dishes The incubator has two independently cont...

Page 10: ...t become depressed during transit covering the recessed plate with rigid card should achieve this 2 1 Docking Station The Incubator is mounted on a docking station built into the carry case which inco...

Page 11: ...if absolutely necessary 2 3 Battery Charging It is recommended to charge the battery fully prior to use To do this remove the power supply unit from the kit and connect it to the charging connection o...

Page 12: ...ed Cable available separately Note The vehicle cigarette lighter charging lead is a specialist product and as such only a genuine Trace2o replacement should be used Replacements are available from any...

Page 13: ...by pushing down on the top whilst holding the rack twisting to the left and then removing the top To re fit align the arrow with either of the two slots in the top of the rack push down and twist loc...

Page 14: ...he incubation cycle from where it was before the power was removed Mode 3 is primarily intended for unattended power failure conditions If Fig 1 0 is displayed and the incubator was in mode 2 then the...

Page 15: ...sed to step through the sequence instead of the left As such the functions of the right chamber will not be described in detail It should be noted that each chamber can be run with a different setting...

Page 16: ...r the left chamber The bottom right of the display will show the current state of the right chamber OFF is shown for illustration purposes only 20 9 C 20 9 C OFF MEM OFF Select Option Restart NewRun 2...

Page 17: ...the right chamber The bottom left of the display will show the current state of the left chamber OFF is shown for illustration purposes only 20 9 C 20 9 C OFF MEM OFF Select Option Restart NewRun 20 9...

Page 18: ...ws the current status of the incubator In the state shown the incubator is in idle mode and not running in either chamber 20 9 C 20 9 C OFF MEM OFF Left Chamber Temperature Right Chamber Temperature L...

Page 19: ...eturn to the menu function screen During the run mode a short press of the middle switch will indicate the battery voltage For best accuracy always check the battery voltage when the heater is off whe...

Page 20: ...seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature will switch on and off indicating the status of the heater To stop or pause...

Page 21: ...iddle switch for 5 seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature will switch on and off indicating the status of the heater...

Page 22: ...then turn back on which will exit this mode and return to the menus without resetting the timer 3 3 8 Incubation cycle time setting Fig 1 6 In this mode the default cycle time can be changed from 24...

Page 23: ...o adjust the temperature press the middle switch The screen will change to Use the left switch to decrease the temperature and the right switch to increase the temperature When the desired temperature...

Page 24: ...ur Compare the displayed temperature on the incubator screen to that shown on the thermometer If there is a difference 0 2 C the calibration should be adjusted as follows Exit the run mode and step th...

Page 25: ...et conditions and must not be submersed in water The incubator chamber is sealed at the bottom so spills will not affect the unit but should be cleaned as soon as possible The Aluminium chamber can be...

Page 26: ...e rechargeable lead acid batteries may be necessary If so these must be replaced with batteries of the same type as specified in section 5 1 and replaced as a pair Should any fault occur please contac...

Page 27: ...d Assistance Trace2o hope that the AquaSafe incubator will give many years of trouble free operation but in the event of a technical problem occurring the AquaSafe incubator is covered by Trace2o Ltd...

Page 28: ...Section 4 AquaSafe Membrane Filtration Instruction Manual Contents...

Page 29: ...CTION 3 PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD 33 SECTION 4 SAMPLING 34 SECTION 5 USE OF BACTERIOLOGICAL MEDIA 36 SECTION 6 ASEPTIC PROCEDURES 37 SECTION 7 PROCESSING SAMPLES FOR COLIFORM ANALYS...

Page 30: ...TRATION APPARATUS 1 Hand Vacuum Pistol Pump 2 Filtrate Flask Waste Beaker 3 Sampling Cup and cable 4 Graduated Aluminium Funnel 5 Membrane Support Holder 6 Sealing Gaskets 7 Glass Sintered Disc Membra...

Page 31: ...and press into place Place the sintered disc smooth side facing upwards into the centre of the gasket Push the other two gaskets into place around the sintered support disc The graduated funnel screw...

Page 32: ...cleansing agent pour the prepared medium carefully into the bottles Replace bottle lids but leave them slightly loose do not fully tighten Sterilise in an autoclave at 121 C for 10 minutes or place b...

Page 33: ...nge to green to indicate moisture penetration Tear open the sachet and remove the silica gel with the forceps If the silica gel is green discard the sachet without using Open a bottle of Sterilised wa...

Page 34: ...ple collection bottles contain dechlorination tablets N B If the sample is analysed immediately on site then it is not necessary to dechlorinate the water Dechlorination Kit The dechlorination tablets...

Page 35: ...ntil the water cools to its original temperature Fill the prepared bacteriological sampling bottle leave a slight air gap at the top and seal the lid tightly Dip Sample Sterilise the sampling cup by i...

Page 36: ...in a water bath for 15 minutes Clean empty media bottles thoroughly before re use Any residues should be washed out with hot water cleaned with a little detergent a small brush can be used if required...

Page 37: ...ignite the methanol TAKE CARE AROUND THE NAKED FLAME Allow the methanol to burn for a few seconds Whilst the methanol is still burning invert the filtration unit and carefully place inside the sample...

Page 38: ...is necessary to dispense pads in the field great care must be taken not to contaminate either the pad dispenser or the cartridge As soon as a cartridge is finished a new one should be attached to the...

Page 39: ...the graduated filter funnel and remove from the base support Sterilise the forceps and allow to cool Using these forceps place a sterile membrane onto the glass membrane support grid side up If the me...

Page 40: ...Connect the hand vacuum pump to the filtration unit base and pump in a controlled fashion to suck the water sample through the membrane...

Page 41: ...le number place date time etc Place the petri dish into the PetriLok cartridge Repeat the process for all the samples and then place the filled rack into the incubator It is important to note that whe...

Page 42: ...hin a few minutes of removing from the incubator as the colours are liable to change on cooling and standing Ignore those colonies that are not yellow e g pink transparent colonies Once the number of...

Page 43: ...s 10 faecal coliforms per 100 mL action is urgently required Raw Waters For source waters and partially treated waters including those which are ground water derived it can be useful to adjust the sam...

Page 44: ...Section 5 AquaSafe Turbidity Tube Instruction Manual...

Page 45: ...turbidity tube from their position in the foam recess at the front of the AquaSafe Kit Align the two halves of the turbidity tube so that the graduations are easily visible then push together the gre...

Page 46: ...tively fill the tube then slowly pour small portions away until the moment that the Secchi marker becomes visible from the top of the tube Hold the tube vertically and identify the water level The tur...

Page 47: ...Section 6 AquaSafe Block Tester Instruction Manual for WSL Range...

Page 48: ...chambers are those into which tablets should be added The chambers are referred to by a number from 1 5 as shown in the above image SECTION 2 PREPARATION Remove the black rubber cap and immerse the en...

Page 49: ...SECTION 3 pH PHENOL RED TABLET Take 1 x Phenol Red tablet directly from the foil and add to chamber 2 SECTION 4 FREE CHLORINE DPD1 TABLET...

Page 50: ...ectly from the foil and add to chamber 4 Recap the block tester firmly ensuring that the arrows on the black rubber cap are pointing towards the coloured overlays Shake vigorously and hold up to the l...

Page 51: ...esult Notes pH values below 6 5 always produce a yellow colouration pH values above 8 4 always produce a red colouration Water samples with low total alkalinity may give incorrect results Turbid sampl...

Page 52: ...and the coloured overlays on chambers 3 and 5 Record the result Combined Chlorine mg L ppm Total Chlorine mg L Free Chlorine mg L Notes Take care not to over shake or aerate the sample Store the reage...

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