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Hyperspectral Imaging System

CM401

User Guide

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Summary of Contents for CM401

Page 1: ...Hyperspectral Imaging System CM401 User Guide ...

Page 2: ...The software may be used or copied only in accordance with the terms of those agreements No part of this publication may be reproduced stored in a retrieval system or transmitted in any form or any means electronic or mechanical including photocopying and recording for any purpose other than the purchaser s personal use without the written permission of ThorLabs Inc ThorLabs Inc 108 Powers Court S...

Page 3: ...ions 17 7 1 Microscope Body Specifications 17 7 2 Sample Holder Specifications 17 7 3 XY Translation Stage Specifications 17 7 4 Objective Specifications 18 7 5 Condenser Specifications 18 7 6 Tunable Bandpass Filter Specifications 19 7 7 Camera Port Specifications 20 7 8 Camera Specifications 20 7 9 Motorized Controller Specifications 21 7 10 Plasma Light Source Specifications 22 Chapter 8 Mechan...

Page 4: ... Current Earth Ground Terminal Protective Conductor Terminal Frame or Chassis Terminal Equipotentiality On Supply Off Supply In Position of a Bi Stable Push Control Out Position of a Bi Stable Push Control Caution Risk of Electric Shock Caution Hot Surface Caution Risk of Danger Caution Laser Radiation Caution Spinning Blades May Cause Harm Page 1 TTN131444 D02 CM401 Chapter 1 Warning Symbols and ...

Page 5: ...roller electronics enclosure detector module or computer There are no serviceable parts in these products DO NOT perform instrumentation maintenance or service except as specifically stated Any modification or servicing of this system by unqualified personnel renders Thorlabs free of any liability Only personnel authorized by Thorlabs and trained in the maintenance of this equipment should remove ...

Page 6: ...des a benchtop controller with external triggers 3 1 Features at a Glance General Microscope Features Microscope body with 7 74 Throat Depth 10X Magnification Plan Fluor Objective Nosepiece with 1 of Fine Z Travel 0 9 NA Condenser Suitable for Brightfield Phase Contrast Darkfield and DIC Differential Interference Contrast Condenser Holder with 1 of Fine Z Travel Two 3 Axis Controllers and Knob Box...

Page 7: ...m 80 ms Wide 50 ms Scientific Camera Specifications Item 4070M GE Sensor Type ON Semiconductor KAI 04070M Monochrome CCD Number of Active Pixels 2048 x 2048 Horizontal x Vertical Pixel Size 7 4 µm x 7 4 µm Imaging Area 15 2 mm x 15 2 mm Horizontal x Vertical Host PC Interface Gigabit Ethernet Vertical Hardware Binningc Continuous Integer Values from 1 to 10 Horizontal Software Binningc Continuous ...

Page 8: ... Repeatability 1 µm Backlash 1 µm Minimum Achievable Incremental Movement 100 nm Minimum Repeatable Incremental Movement 200 nm Included Software ThorImageLS 3 1 with Lifetime Support a The bandpass width increases linearly with wavelength b The switching speed depends upon the bandpass width setting and the initial and final wavelength c Camera Frame Rate is impacted by the Vertical Hardware Binn...

Page 9: ...001 2 High Power Light Source with Liquid Light Guide HPLS343 1 KURIOS Filter Controller with Optical Head Cable KURIOS 1 10X Nikon Plan Fluor Objective N10X PF 1 Nikon D CUD Universal Condenser 0 90 NA CSC1002 1 Rigid Stand on PLS XY Translation Stage 1 BNC to SMA Cable 2 Auxiliary Cable 8050 CAB1 2 M6 Socket Head Cap Screw 16 1 4 20x1 2 Socket Head Cap Screw 14 M6 Flat Washer 10 ThorCam Camera S...

Page 10: ...ll culture incubators hoods etc Minimize air drafts as it can result in movement of your imaging system This can result in problems related to focusing on your sample Furthermore drafts can also lead to particulate contamination that can damage your hardware and or introduce artifacts into your imaging field Operate in light controlled environments to make sure of best quality images Make sure tha...

Page 11: ...horized personnel should install this system A Mounting the System 1 Mount the microscope body onto the optical table 2 Remove the shipping plates on the microscope body and rigid stand 3 Mount the objective camera condenser onto the microscope body Rev A June 27 2017 Page 8 CM401 Chapter 4 Getting Started ...

Page 12: ...able B Computer Please refer to the computer s user manual for installation and operating instructions ThorImageLS 3 1 software comes preinstalled on the computer Install the ThorCam camera software on the computer see the relevant user guide for details Page 9 TTN131444 D02 CM401 Chapter 4 Getting Started ...

Page 13: ...arm to the other MCM3000 Controller Box 5 Use the Liquid Light Guide to connect the LLG PORT of Light Source HPLS343 to the Illumination Module 6 Use the Optical Head cable to connect the OPTICAL HEAD port of Filter Controller to the KURIOS filter 7 Use an USB cable to connect the USB port of Filter Controller to the computer 8 Use a BNC to SMA cable to connect the TRIGGER OUT port of Filter Contr...

Page 14: ...ted devices and then click OK 3 Use the Z Control panel to focus on the sample 4 In the tab make sure that the Camera Control panel Kurios Filter panel and Z Control panel are visible If you cannot see a panel click Edit Settings Display Options and select the desired panel Click OK Note The Kurios Filter panel appears only if you select ThorKurios as the Spectrum Filter in Hardware Connections wi...

Page 15: ...elength 730 nm of the KURIOS filter The ThorImageLS application supports three different bandwidth modes WIDE MEDIUM and NARROW 6 Enter the Start value for the Wavelength value for example 420 nm and then click in the Capture Setup Panel 7 In the Camera Control panel change the Exposure value until you see the desired brightness on the image Record the desired Exposure value Start Exposure on a pa...

Page 16: ...Sequence window appears 11 Choose an arbitrary value between the Start and Stop wavelengths In Kurios Filter panel enter the arbitrary value for the Wavelength 12 In the Camera Control panel change the Exposure value until you see the desired brightness on the image 13 In the Hyperspectral Sequence window click to save the point 14 Repeat Steps 11 13 for different wavelengths between the Start and...

Page 17: ... select Hyperspectral for Capture Mode This option is only visible if you select Scientific Camera as the Image Detector and ThorKurios as the Spectrum Filter in the Hardware Connection window Select Raw Data Capture if you want to save the experiment as a raw and hdr files Deselect the option to save every image as a tiff file Rev A June 27 2017 Page 14 CM401 Chapter 5 Capturing an Image ...

Page 18: ...captured experiment A new slider appears that shows the spectral wavelength of the current image and its index For information about the hyperspectral acquisition and camera settings expand the Info panel Page 15 TTN131444 D02 CM401 Chapter 5 Capturing an Image ...

Page 19: ...wide Contacts Chapter on Page 27 for details 6 1 Cleaning Refer to the user guide of the individual product for cleaning instructions WARNING Disconnect the power supply before cleaning the unit to prevent any injuries CAUTION DO NOT expose the unit to spray liquids or solvents This may damage the unit Use a damp lint free cloth to clean the unit Rev A June 27 2017 Page 16 CM401 Chapter 6 Maintain...

Page 20: ... Stage Specifications Specification Value Travel Range 25 4 mm 1 Bidirectional Repeatability 1 µm Backlash 1 µm Minimum Achievable Incremental Movement 100 nm Minimum Achievable Repeatable Movement 200 nm Velocity Max 7 mm s Acceleration 11 mm s2 Cable Length 6 1 8m Load Capacity Stage Mounted to Vertical Rail Recommended 10 lbs 4 5 kg Maximum 10 lbs 4 5 kg Stage Mounted to Horizontal Rail Recomme...

Page 21: ...200 mm Coverslip Correction 0 17 mm Barrel Diameter 30 0 mm Length 48 7 mm Threading M25 x 0 75 7 5 Condenser Specifications Specification Value Item CSC1002 Manufacturer Part MBL99005 Air or Oil Immersion Air Condenser Type Achromatic Numerical Aperture NA 0 9 Aperture Adjustment Yes Working Distance 2 3 mm Recommended Objective Magnification 2X 100X Dovetail Male D3N Internal Turret Slots Number...

Page 22: ...gle of Incidence Field of View 6 Wavelength Uniformity FWHM 4 over Clear Aperture Damage Threshold Pulsed ns Pulsed fs CW 0 1 J cm2 0 02 J cm2 532 nm 76 Hz 100 fs Ø162 µm 0 8 W cmd 532 nm Ø0 471 mm Filter Head Dimensions 52 6 mm x 52 6 mm x 78 5 mm 2 07 x 2 07 x 3 09 Filter Head Front Rear Threading SM1 1 035 40 Internal Threads Filter Head Mounting Options 8 32 M4 Tap for Post Mounting 3 Places 4...

Page 23: ...fficiency 52 at 500 nm Number of Taps Software Selectable Single Dual Exposure Time 0 to 1000 s in 1 ms Incrementsb CCD Pixel Clock Speed 20 or 40 MHz Analog to Digital Converter Gain 0 to 1023 Steps 0 036 dB Step Optical Black Lamp 0 to 1023 Steps 0 25 ADU Step c Vertical Hardware Binningd Continuous Integer Value from 1 to 10 Horizontal Software Binningd Continuous Integer Value from 1 to 10 Reg...

Page 24: ...Hz Intel Core i5 or Core i7 Memory RAM 8 GB Hard Drive 500 GB SATA Solid State Drive SSD g Graphic Card Dedicatedh Adapter with 256 MB RAM Power Supply 600 W Motherboard One Unused PCIe x 1 Slot Connectivity USB or Internet Connectivity for Driver Installation f Intel Core i3 processors and mobile versions of Intel processors may not satisy the requirements g We recommend a SSD for reliable stream...

Page 25: ...ral Computer Connection USB 2 0 Housing Dimensions W x D X H 97 3 mm x 50 8 mm x 73 6 mm 3 83 x 2 00 x 2 90 7 10 Plasma Light Source Specifications Specification Value Item HPLS343 Physical Dimensions L x W x H 14 23 x 7 40 x 7 87 361 5 mm x 188 0 mm x 200 0 mm Operating Temperature 10 to 30 C Open and Closed Loop 10 to 35 C Eco Mode Storage Temperature 15 to 70 C Power Supply 90 VAC to 264 VAC 47...

Page 26: ... Operating Wavelength Range 340 nm to 800 nm a Measured at the output of the liquid light guide LLG when bulb and LLG are at start of life b Typical value which includes the photo induced darkening of the liquid in the LLG that occurs naturally during operation as a result of exposure to UV radiation c Typical standard deviation for measurements taken over an hour at the output of the LLG d Averag...

Page 27: ... 0 82 20 8 mm 0 63 16 0 mm Working Distance 7 74 196 5 mm Throat Depth 14 81 376 1 mm 3 31 84 1 mm Imperial and Metric Compatible Baseplate D1N Female Dovetail Compatible with Cerna Components Microscope Without Sample Holder 15 18 385 6 mm 3 27 83 1 mm 9 84 249 9 mm Rev A June 27 2017 Page 24 CM401 Chapter 8 Mechanical Drawing ...

Page 28: ... 4 0 MP Monochrome Scientific CCD Camera Tunable Filter Selectable Bandpass Ø20 mm 420 730 nm Removable Cover to Access Filter Input Channel for Ø3 mm LLG Epi Illuminator Module for Filter Cube Male Female D1N Dovetails Nikon Objective N10X PF Microscope With Sample Holder Page 25 TTN131444 D02 CM401 Chapter 8 Mechanical Drawing ...

Page 29: ...semblies to be built into a unit by the user e g OEM laser driver cards Components Mechanics and optics Left over parts of units disassembled by the user PCB s housings etc If you wish to return a Thorlabs unit for waste recovery please contact Thorlabs or your nearest dealer for further information 9 1 Waste Treatment is Your Own Responsibility If you do not return an end of life unit to Thorlabs...

Page 30: ...ada and South America Thorlabs Inc sales thorlabs com techsupport thorlabs com Europe Thorlabs GmbH europe thorlabs com France Thorlabs SAS sales fr thorlabs com Japan Thorlabs Japan Inc sales thorlabs jp UK and Ireland Thorlabs Ltd sales uk thorlabs com techsupport uk thorlabs com Scandinavia Thorlabs Sweden AB scandinavia thorlabs com Brazil Thorlabs Vendas de Fotônicos Ltda brasil thorlabs com ...

Page 31: ...www thorlabs com ...

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