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5.3  Troubleshooting Chart  - Freezing Chamber (continued)

Problem Probable 

Cause 

Remedy 

Fan is not operating. 

Faulty limit switch.

Replace switch.

Fan has not been energized at the controller.

Energize fan at controller. 

Fan is jammed or motor is defective. 

Mechanically free fan and/or replace fan motor.  

Inability to sufficiently

LN2 transfer hose connected to vent side of supply tank.

Connect transfer hose to liquid side of 

cool specimens.

supply tank. 

Controller is improperly set.

Readjust controller setting (add Cool+).

Insufficient liquid nitrogen pressure at freezing chamber.

Increase liquid nitrogen supply pressure

to 22 PSIG as measured at the freezing chamber.

Defective limit switch. 

Replace limit switch.  

Nitrogen gas is escaping

Liquid nitrogen pressure supply is too high.

Reduce liquid nitrogen pressure to 22 PSIG.

from pressure relief valve 

Pressure relief valve is defective. 

Replace pressure relief valve. 

at rear of freezing chamber. 

Heater in freezing chamber 

Heater has not been energized at controller.

Energize heater.

does not operate. 

Element is faulty.

Replace faulty heating element.

Heater relay is faulty.

Replace faulty heater relay.

Wiring connections are faulty. 

Check wiring harness for proper 

connector seating at controller.  

Models 1010 and 1011 ________________________________________________________________________Maintenance

5 - 3

Summary of Contents for Cryomed 1010

Page 1: ...Cryomed Model 1010 1011 Programmable Freezing System Operating and Maintenance Manual Manual No 7001010 Rev 3 ...

Page 2: ... manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Forma makes no representations or warranties with respect to this manual In no event shall Thermo Forma be held liable for any damages direct or inciden tal arising out of or related to the use of this manual MANUAL NUMBER 7001010 3 2 5 01 Updated format to Quark ccs 2 193...

Page 3: ...cedimientos asociadas con este simbulo Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Risques potentiels liés à l énergie L équipement en entretien ou en maintenance doit être éteint et mis sous clé pour éviter des blessures possibles Geräte an denen Wartungs oder Servicearbeiten durchgeführt werden müssen abgeschaltet und abgeschlossen wer den ...

Page 4: ...Models 1010 and 1011__________________________________________________________________________Safety iii ...

Page 5: ... 4 Operation 4 1 4 1 Control Panel Operation 4 1 a Control Section 4 1 b Mode Select 4 1 c System Status 4 2 d TC Scanner 4 2 e Program Display 4 2 f Display 4 2 g Prog Adv 4 3 h Numerical and Special Functions 4 3 4 3 System Operation 4 3 4 4 Initiating a Freezing Run 4 4 4 5 factory Pre set Programs 4 4 4 6 Programming the Controller 4 5 4 7 Running the Program in the Unlimited Access Manual Mod...

Page 6: ...ed programmable controller that provides precise automatic temperature control for a choice of freezing chambers Six standard factory set programs provide ease of operation for common freezing operations Additionally nine programs that may be operator programmed are available to address the user s specific freezing applications Each program consists of nine sections for continuous temperature prog...

Page 7: ... direction by a crystal controlled stepper motor The mechanical construction of the flatbed recorders is based on modules Thus amplifiers stepper motor printed cir cuit assembly and input modules are easy to add and easy to change The printer is configured for 0 10 Volt scale fixed Chart Speed 1 2 per minute fixed The solid mechanical construction allows the use of this recorder for all applicatio...

Page 8: ... allocated for tank replace ment Caution The freezing chamber must not be posi tioned above the controller or recorder Such positioning will result in condensate dripping into the electronics and will cause serious damage to the internal circuitry Caution The gaseous nitrogen vent located at the rear of the chamber must not be obstructed obstruction of the vent will seriously interfere with normal...

Page 9: ...ecorder operation refer to the supplemental recorder manual 3 4 Connecting the Liquid Nitrogen Transfer Hose Warning Some liquid nitrogen supply tanks con tain a pressure capable of supplying liquid or gaseous nitrogen at up to 235 PSIG Under such conditions change the supply tank to one with a 22 PSIG setting to provide compatibility Using a 7 8 open end or an adjustable wrench connect the transf...

Page 10: ...nner operation for sample only chamber only or sample and chamber alternately and relay temperature information to strip chart recorder 5 Digital LED display of selected program section num ber hold time elapse time and setpoint temperatures 6 Four indicators allow user to toggle display of pro grammed actual chamber or sample temperature 7 Up and down arrow keys allow user to advance or retreat t...

Page 11: ...on troller operation will resume CONTROL NAME FUNCTION COOL Controls the liquid nitrogen solenoid valve circuit for the injection of additional LN2 for accelerated freezing rates as follows Automatic Operation Enables COOL circuit when con troller is in IDLE mode This will be enabled only if it has been programmed into the section being viewed The red LED illuminates to indicate that the COOL cir ...

Page 12: ...FUNCTION SAMP Selects the Sample thermocouple as the temperature to be sent to the recorder The red LED illuminates when the controller is sending out Sample temperature to recorder CHAM Selects the Chamber thermocouple as the temperature to be sent out to the recorder The red LED illumi nates when the controller sending out chamber temperature to the recorder SCAN Tells computer to scan both ther...

Page 13: ...gen to the freezing chamber Additionally it supplies temperature information to the strip chart recorder The freezing cycle consists of the following steps and oper ations Note Typically the biological sample is mixed with a solution of cryoprotective agent and placed in a plastic cryoampule 1 STARTING TEMPERATURE Sample temperature prior to being loaded into the freezing chamber The sample is usu...

Page 14: ...er the pen to the graph paper 4 Calibrate the recorder as follows a Press CHAM in the TC Scanner section of the controller The recorder pen should move to zero on the strip chart recorder If it does not dial it to zero using the black knob on the recorder b Press SAMP in the TC Scanner section of the controller The recorder pen should move to 180 C If it is out of calibration adjust using the set ...

Page 15: ...shown at the right Nine programs each consisting of nine sections are user programmable Each memory section is designed to hold infor mation necessary to perform a single task Each freezing task can consist of only one of the following 1 A HOLD time while maintaining a chamber tempera ture setpoint 2 A WAIT function while maintaining a chamber tem perature setpoint 3 A RATE C MIN temperature incre...

Page 16: ... setting which is recommended to be a sample switch point Programming Section 3 Press 3 0 0 ENT This is selects a rate of 30 per minute Temp Drop Press 6 0 COOL ENT This selects a temperature drop of 60 C Programming Section 4 Press 1 5 0 ENT This selects a rate of 15 degrees per minute End of Phase Change Press 1 2 COOL ENT This is the end of Phase Change tempera ture which is generally 8 to 10 d...

Page 17: ...am only to the next section 4 9 Programming the Access Code The access code 1 2 3 was factory set prior to ship ment of the Model 8018 If the user operator desires to change the access code perform the following steps Example Change access code to 4 5 6 Depress keys in this sequence PROG 1 2 3 ENT CLR 4 5 6 ENT Note If the new three digit access code is not entered within ten seconds the controlle...

Page 18: ...int or start of Phase Change Temperature Heat of Fusion Plateau Temperature Make the following connections Note Refer to the the serial tag on the rear of the controller for specific electrical requirements 1 POWER TO THE CONTROLLER With the controller power switch set to the OFF position connect the con troller power cord to a grounded power source 2 Chamber thermocouple input Provides chamber th...

Page 19: ...arameters that were programmed into the current section They are displayed in a hexadecimal format the base sixteen number system The next three lines show the current Control temper ature Chamber temperature and Sample temperature in degrees Centigrade The last line shows the current system sta tus This is also a hexadecimal number with each bit represent ing a different condition The following t...

Page 20: ...0A0 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 D0B0 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 D0C0 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 D0D0 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 D0E0 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 D0F0 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 D100 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 D110 06 00 00 00 00 06 00 00 0...

Page 21: ... data file would appear as follows if the D command were used with a CRT terminal ON LINE D 10000000022B0600000000060000000006000000B1 1000100000060000000006000000000600000000CE 1000200006000000000600000000060000000006B8 1000300600000000060000000006000000000600AE 10004000000000060000000006000000000600009E 10005000000006000000000600000000060000008E 10006000000600000000060000000006000000007E 1000700...

Page 22: ...he component to open flame or other possible hazard Caution Do not use strong alkaline or caustic agents Stainless steel is corrosion resistant not corrosion proof Do not use chlorinated solvents or other halogens on stainless steel as they will cause rusting and pitting 1 The freezing chamber can be easily cleaned with an appropriate disinfectant detergent Refer to manufactur er s directions for ...

Page 23: ...N ERR 6 Non volatile RAM Test failed Wait unit will clear itself All user programmed memory must be This could appear in a low voltage reprogrammed Factory set programs will not be lost or brown out situation ERR 7 This is the result of an electrical Turn unit off wait one minute turn unit back on if ERR 7 disturbance reappears call the factory The unit must be returned to the factory for repair 5...

Page 24: ...ncrease liquid nitrogen supply pressure to 22 PSIG as measured at the freezing chamber Defective limit switch Replace limit switch Nitrogen gas is escaping Liquid nitrogen pressure supply is too high Reduce liquid nitrogen pressure to 22 PSIG from pressure relief valve Pressure relief valve is defective Replace pressure relief valve at rear of freezing chamber Heater in freezing chamber Heater has...

Page 25: ... Internal dimensions 6 x 11 38H x 5 F B 15 2cm x 28 9cm x 12 7cm 9 W x 12 H x 9 F B 22 9cm x 30 5cm x 22 9cm 13 W x 13 H x 13 F B 33cm x 33cm x 33cm Volume 353 cu in 972 cu in 2197 cu in Weight 30 lbs 13 kg 51 lbs 23 kg 90 lbs 41 kg Electrical supplied by controller 95 125VAC 50 60 Hz Same Same 190 250VAC 50 60 Hz 8022 8023 8024 8025 8026 8027 Fan Diameter 4 10 2cm 8 20cm 8 20 3cm of wings 4 5 5 R...

Page 26: ...k Connect SPDT 4000381 Thermocouple Sensor Sample 2Ml Model 8024 Stock No Description 4000400 Transfer Hose 4 Ft 100080 Fan Blade 8 CW 103036 Gasket Silicone P Style 12x11 ID 121047 Latch Handle Freezing Chamber 121048 Cam Latch Freezing Chamber 156067 Fan Motor 1 50 HP 1550 RPM 189801 Strike Plate Teflon 250093 Solenoid Brass 1 4 FPT 120V NC 251004 Pressure Relief Valve 1 4 MPT 360193 Switch Push...

Page 27: ... SPDT 4000385 Thermocouple Sensor Sample 2Ml Model 8027 Stock No Description 4000401 Transfer Hose 6 Ft 100080 Fan Blade 8 CW 103037 Gasket Silicone P Style 16x13 ID 121047 Latch Handle Freezing Chamber 121048 Cam Latch Freezing Chamber 156083 Fan Motor 1 80 HP 1500 SP 3 3 189801 Strike Plate Teflon 250094 Solenoid Brass 1 4 FPT 220V NC 251004 Pressure Relief Valve 1 4 MPT 360193 Switch Push butto...

Page 28: ...nty period The Thermo Forma Service Department must give prior approval for return of any components or equipment At Thermo Forma s option all defective parts must be returned to Thermo Forma postage paid and replacement parts are shipped FOB destination THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER WRITTEN ORAL OR IMPLIED NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A ...

Page 29: ...Thermo Forma Millcreek Road P O Box 649 Marietta Ohio 45750 U S A Telephone 740 373 4763 Telefax 740 373 4189 ...

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