ThermoFisher Scientific Vanquish VH-D10, Operating Manual

Page: 67 / 196

5 • Installation
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 67
5.6.4 Flow Connections to the Flow Cell
Set up the flow connections to the flow cell when the flow cell is
installed to the detector.
NOTICE
Flow cells are highly sensitive to contamination, clogging and
high backpressures. Even if the pressure exceeds the upper limit for a
very short time only, the flow cell may be permanently damaged.
Observe the following notes when connecting the flow cell to the system
flow path:
•
Backpressures that exceed the specified maximum pressure limit of
the flow cell can destroy the flow cell. Never expose the flow cell to
excessive backpressure. When connecting a component in the flow
path after the flow cell, observe the specified backpressure for the
flow cell.
•
Avoid clogging of the flow cell or waste line.
•
Improperly set up flow connections can lead to leaks on the flow cell
or even destroy the flow cell.
•
Use only the waste line and clean Viper capillaries which were
provided for the flow cell and which have been properly protected
by their cap before. Follow the instructions on connecting the inlet
and outlet ports of the flow cell below.
• Use the flow cell only with a column or a filter frit connected in the
flow path before the flow cell.
•
Do not invert the flow cell inlet and outlet for normal flow cell
operation. Inverting the flow connections is only allowed for the
back-flushing procedure that is described in the manual.
•
Do not install switching valves or fraction collectors downstream of
the flow cell, as operating those under flow will result in pressure
spikes that can destroy the flow cell.
• Use only the waste line connection that is provided for your
detector. Connect the waste line to the flow cell only as described in
the manual. Do not discharge waste from the flow cell through the
open leakage drain system of the Vanquish system.
In addition, observe the guidelines for proper connection of the flow cell
in
section 5.6.1, page 62 .