ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 96 / 188

6 • Operation
Page 96 Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
6.5 Preparing the Device for Operation
This section gives information on any additional steps that are required
to prepare the device for operation and sample analysis.
Before Operating the Device for the First Time
Prepare the device for the first-time operation, observing the following:
NOTICE
Flush the system flow path thoroughly before operating the device for
the first time:
• When you install devices or components to the system, always flush
them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the
Vanquish
System Operating Manual .
• When operating the device with a flow cell that was stored, the flow
cell may be filled with solvent. Use solvents that are miscible with
this solvent, or use an appropriate intermediate solvent.
Before Starting Sample Analysis
Before starting an analysis:
• Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the analysis.
• Verify that the doors of all modules in the Vanquish system are
closed.
• Observe the guidelines for use of flow cells in Guidelines for Use of
Flow Cells (
}
page 98) .
• Make sure that the chromatography system is properly equilibrated
(see further down).
System Equilibration
System equilibration should include the following operations:
• Purging the pump ( all channels, including those not used for the
application)
• Flushing the entire chromatography system with the starting solvent
to rinse out any solvent from a previous analysis run