6
•
Operation
Page 88
Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Operating Manual
6.5
Preparing the Device for Operation
This section gives information on any additional steps that are required
to prepare the device for operation and sample analysis.
Before Operating the Device for the First Time
Prepare the device for the first-time operation, observing the following:
NOTICE
Flush the system flow path thoroughly before operating the device for
the first time:
•
When you install devices or components to the system, always flush
them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the
Vanquish
System Operating Manual
.
•
New flow cells are dry when shipped. When operating the detector
with a flow cell that was stored, it is filled with isopropanol. Use
solvents that are miscible with isopropanol. If they are not, use an
appropriate intermediate solvent.
•
To remove the isopropanol from the flow cell:
¨
Verify that a column or a filter frit is connected in the flow path.
If you use isopropanol/water as solvent, verify that the column is
not connected in the flow path.
¨
Have the pump deliver flow for a short time.
•
Verify that any air bubbles are completely flushed out of the system
flow path.
•
Perform a wavelength validation. If the validation fails, perform a
wavelength calibration.
Before Starting Sample Analysis
Before starting an analysis:
•
Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the analysis.
•
Verify that the doors of all modules in the Vanquish system are
closed.
•
Observe the guidelines for use of flow cells in
.
•
Make sure that the chromatography system is properly equilibrated
(see further down).