Thaw, vortex, and centrifuge an RNase P plate
IMPORTANT!
Expose the RNase P plate to room temperature for no more than
45 minutes, inclusive of thawing and preparation time.
After thawing, the RNase P plate cannot be refrozen.
1.
Remove the RNase P plate from the freezer, then thaw the plate in its packaging.
• Thaw the plate for approximately 5 minutes.
• Use the plate within 30 minutes of thawing.
2.
Confirm that the bench, vortex, and centrifuge are clean. Before use, wipe the
vortex and centrifuge using a lint
‑
free tissue.
3.
While wearing powder‐free gloves, remove the plate from its packaging.
4.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 ×
g
for 2 minutes.
5.
Confirm that the liquid in each well is at the bottom of the well and free of
bubbles. If it is not, centrifuge the plate again.
IMPORTANT!
Keep the bottom of the plate clean. Fluids and other
contaminants on the bottom of the plate can contaminate the sample block and
cause an abnormally high background signal.
Perform RNase P verification
1.
In the home screen, tap
(Settings)
4
Maintenance and service
4
RNase P
verification
.
2.
Follow the instructions on the screen to start the verification.
3.
Load the plate into the instrument.
CAUTION!
The instrument should be used by trained operators who have been
warned of the moving parts hazard.
4.
Tap
Start
.
Chapter 7
Calibrate and verify instrument performance
Perform verification using RNase P plates
7
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QuantStudio
™
6 Pro and 7 Pro Real-Time PCR Systems User Guide
