IMPORTANT!
Keep the bottom of the plate clean. Fluids and other
contaminants on the bottom of the plate can contaminate the sample block and
cause an abnormally high background signal.
Add a custom dye to the instrument
1.
In the home screen, tap
(Settings)
4
Maintenance and
service
4
Calibration
4
Custom dye
.
The
Custom Dye
screen is displayed.
2.
Tap
Add custom dye
.
3.
Tap the
Custom Dye Name
field, then enter a name.
IMPORTANT!
·
Do not use a system dye name for a custom dye name.
·
Dye names are spacing sensitive and cannot contain special characters.
4.
Select the type of dye.
Option
Description
Reporter
The dye works in conjunction with a quencher dye to report an increase
of PCR product.
Quencher
The dye suppresses the fluorescence of a reporter dye until
amplification of PCR product.
Both
The dye can be used as a reporter dye or a quencher dye.
5.
Tap
Save
.
IMPORTANT!
The custom dye must also be added to the desktop software library
before creating, running, or analyzing experiments that use custom dyes.
Perform a custom dye calibration
1.
In the instrument home screen, tap
(Settings)
4
Maintenance and
service
4
Calibration
4
Custom dye
.
The
Custom Dye
screen is displayed.
2.
Tap a custom dye.
3.
Review the information, then tap
Update
.
4.
Enter the calibration temperature.
Note:
The default calibration temperature is set to 60°C.
5.
(Optional)
Tap
Reagents
, then enter the reagent information.
Chapter 7
Calibrate and verify instrument performance
Calibrate custom dyes
7
102
QuantStudio
™
6 Pro and 7 Pro Real-Time PCR Systems User Guide
