• Load the tip boxes, then remove the tip box covers.
Do not stack the tip boxes.
• Place the OpenArray
™
Plate in the plate holders.
• Clear the instrument deck, empty and replace the waste bin, and close the instrument door.
For more information on setting up the instrument deck, see “Set up the system”
.
• Allow the instrument to perform a self-test if the run is being started after the software is launched
(see “Perform a system self-test”
).
A self-test is performed only if
Full Run
is selected after the software is launched. It is not
performed before each run.
• Prepare the QuantStudio
™
12K Flex OpenArray
™
Accessories Kit materials that are required to seal
the OpenArray
™
Plate.
IMPORTANT!
OpenArray
™
Plates must be sealed immediately after loading. For more information,
see the user guide or application guide for your assay.
Configure the run—quick run workflow
Configure the run with sample plate information
The default radio button on the
Quick Run
screen (
Load with sample plate information
or
Load
without sample plate information
) is set in the preferences (see “Set the general settings”
).
A sample plate file (384-well CSV file)
is
required. A TPF or SPF file
is
required. See “File formats”
.
Navigate to the
Quick Run
screen. Ensure that the
Load with sample plate information
radio button is
selected.
1.
In the
Quick Run
screen, in the
Sample plate
section, click
Choose File
.
2.
Navigate to the location of the CSV file, then select the file.
The name of the file is displayed in the
Sample plate
field.
3.
In the
OpenArray plate name and position
section, click
Choose File
for each OpenArray
™
Plate.
Four TPF or SPF files can be imported.
The name of the TPF or SPF file is displayed in the position of the OpenArray
™
Plate.
4.
Click a section of the sample plate to change the corresponding OpenArray
™
Plate.
Note:
The first section of the sample plate is selected. Subsequent sections are selected
automatically.
Chapter 6
Set up and start the run with the quick run workflow
Configure the run—quick run workflow
6
QuantStudio
™
12K Flex OpenArray
™
AccuFill
™
System User Guide
43
