ThermoFisher Scientific QuantStudio 12K Flex OpenArray AccuFill System, User Manual

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Proceed to “Export plate files” on page 38 .
Set up a 384-well sample plate
If the sample plate file was imported, the sample names are displayed. The sample plate layout defined
in the sample plate file can be edited.
If the sample plate file was not imported, the samples must be added manually.
The plates are color-coded for the position on the OpenArray
™
Plate based on how the experiment is
configured (see “Configure the experiment design to map the plates” on page 25 ).
1.
In the Map Plates pane, add the sample name in the 384-well plate.
Click and drag to copy and paste sample names across a row or down a column.
Note: The sample names are copied. They are not automatically filled. For example, if Sample 1
is the first sample name, all of the wells that are filled by the click and drag feature are Sample 1 .
They are not named sequentially, for example, Sample 1 , Sample 2 , Sample 3 .
2.
(Optional) Click Import to import a sample plate file (CSV format).
A sample plate file can be imported from the Map plates pane if it was not imported from the
Configure design pane.
Note: If a sample plate file is imported from the Map plates pane, the information will overwrite
any information from files that were imported from the Configure design pane.
3.
(Optional) Click Rotate data .
The 384-well plate can be placed inside the OpenArray
™
AccuFill
™
Instrument in only one
orientation. If the samples were added to the physical 384-well plate in the incorrect orientation,
the physical 384-well plate cannot be rotated when it is placed in the instrument for the sample
plate file to match. The option to rotate the samples in the sample plate file allows the 384-well
plate to be used in the correct orientation for the instrument with a matching sample plate file.
The
Rotate data feature is available only in the Map Plates screen if a 384-well sample plate file
was imported. It is not accessible in the full run workflow.
The layout of the plate is rotated so that the A1 position moves to the P24 position, the A2 position
moves to the P23 position, and so on.
Chapter 5
Map plates
Set up a 384-well sample plate
5
QuantStudio ™ 12K Flex OpenArray ™ AccuFill ™ System User Guide
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